[1]张慧,马连杰,杭晓宁,等.不同轮作模式下稻田土壤细菌和真菌多样性变化[J].江苏农业学报,2018,(04):804-810.[doi:doi:10.3969/j.issn.1000-4440.2018.04.013]
　ZHANG Hui,MA Lian-jie,HANG Xiao-ning,et al.Changes of soil bacterial and fungal diversity in paddy soils under different rotation patterns[J].,2018,(04):804-810.[doi:doi:10.3969/j.issn.1000-4440.2018.04.013]
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不同轮作模式下稻田土壤细菌和真菌多样性变化()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年04期
页码:
804-810
栏目:
耕作栽培·资源环境
出版日期:
2018-08-25

文章信息/Info

Title:
Changes of soil bacterial and fungal diversity in paddy soils under different rotation patterns
作者:
张慧; 马连杰; 杭晓宁; 朱金山; 卢文才; 廖敦秀
（重庆市农业科学院农业资源与环境研究所，重庆401329）
Author(s):
ZHANG Hui; MA Lian-jie; HANG Xiao-ning; ZHU Jin-shan; LU Wen-cai; LIAO Dun-xiu
(Institute of Agricultural Resources and Environment, Chongqing Academy of Agricultural Sciences, Chongqing 401329)
关键词:
稻田; 轮作; 土壤细菌; 土壤真菌
Keywords:
paddy; crop rotation; soil bacteria; soil fungi
分类号:
S154.31
DOI:
doi:10.3969/j.issn.1000-4440.2018.04.013
文献标志码:
A
摘要:
为探讨不同轮作模式对稻田土壤微生物的影响，设置水稻-小麦、水稻-油菜、水稻-绿肥、水稻-冬闲4个处理，于水稻收获后采集土壤样品，提取基因组 DNA，构建文库，利用Illumina Mi Seq高通量测序技术研究土壤细菌和真菌的丰富度、多样性指数以及门和属水平上的群落结构变化。从12个土壤样品中共获得24 537个细菌OUT 和5 882个真菌OTU，其中，变形菌门、绿弯菌门、酸杆菌门、放线菌门、芽单胞菌门为土壤优势细菌门，子囊菌门和担子菌门为优势真菌门。从门的分类水平看，不同轮作模式土壤中细菌和真菌群落各门类组成的比例发生改变。属水平的分析结果也表明，不同轮作模式土壤中细菌前20个优势属的丰度和真菌前10优势属的丰度同样发生明显改变。不同轮作作物改变了土壤中有益微生物的比例，水稻-绿肥和水稻-冬闲处理有益微生物比例高于其他处理。随着轮作作物的改变，土壤生态系统中细菌和真菌群落的组成比例发生了变化。
Abstract:
Different paddy field soils were used as materials to investigate the effects of different rotation patterns on soil microbes. A total of four treatments including rice-wheat, rice-rapeseed, rice-green manure, and paddy-free were set up. Soil samples were collected after rice harvest, genomic DNA was extracted, libraries were constructed, and high-throughput sequencing of Illumina Mi Seq was used to study the richness, diversity index, and changes in community structure at the phyla and genus levels of soil bacteria and fungi. A total of 24 537 bacterial OUTs and 5 882 fungal OTUs were obtained from 12 soil samples. Among them, Proteobacteria, Chloroflexi, Acidobacteria, Actinobacteria and Gemmatimonadetes were the dominant soil bacteria. Ascomycota and Basidiomycota conjugated bacteria were the dominant fungal phyla. From the perspective of the classification of phyla, the proportions of the composition of the bacteria and fungal communities in the soil also changed. The genus level analysis results also showed that the abundance of bacteria in the first 20 dominant genus and the abundance of fungus in the top 10 dominant genus also changed significantly. Different rotation crops changed the proportion of beneficial microorganisms in the soil, and the proportion of beneficial microorganisms under rice-green manure and paddy-free treatments was higher than that under other treatments. As rotation crops changes, the ratio of the composition of the bacterial and fungal communities in the soil ecosystem changes.

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备注/Memo

备注/Memo:
收稿日期：2018-04-13 基金项目：国家重点研发计划项目(2016YFD0300907)；国家自然科学基金青年项目(41401594)；重庆市科研院所绩效激励引导专项(cstc2017jxjlX0002)；重庆市基本科研业务费项目(2016cstc-jbky-00516、2016cstc-jbky-00517) 作者简介：张慧 (1989-)，女，江苏睢宁人，硕士，助理研究员，主要从事土壤微生物方面的研究。（E-mail）1501380987@qq.com 通讯作者：廖敦秀，（E-mail）664852751@qq.com
更新日期/Last Update: 2018-09-04