[1]管礼麟,马媛,杨丹茹,等.绵羊肺炎支原体P208蛋白质的分子特征分析及免疫原性[J].江苏农业学报,2018,(01):81-86.[doi:doi:10.3969/j.issn.1000-4440.2018.01.012]
　GUAN Li-lin,MA Yuan,YANG Dan-ru,et al.Study on molecular characterization and immunogenicity of Mycoplasma ovipneumoniae P208 protein[J].,2018,(01):81-86.[doi:doi:10.3969/j.issn.1000-4440.2018.01.012]
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绵羊肺炎支原体P208蛋白质的分子特征分析及免疫原性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年01期
页码:
81-86
栏目:
畜牧兽医·水产养殖
出版日期:
2018-02-25

文章信息/Info

Title:
Study on molecular characterization and immunogenicity of Mycoplasma ovipneumoniae P208 protein
作者:
管礼麟; 马媛; 杨丹茹; 杨发龙
（西南民族大学生命科学与技术学院，四川成都610041）
Author(s):
GUAN Li-lin; MA Yuan; YANG Dan-ru; YANG Fa-long
（College of Life Science and Technology, Southwest Minzu University，Chengdu 610041，China）
关键词:
绵羊肺炎支原体; P208蛋白; 分子特征; 免疫原性
Keywords:
Mycoplasma ovipneumoniae; P208 protein; molecular characterization; immunogenicity
分类号:
858.26
DOI:
doi:10.3969/j.issn.1000-4440.2018.01.012
文献标志码:
A
摘要:
为了研究绵羊肺炎支原体P208蛋白质的结构及功能，本试验在对p208基因进行生物信息学分析的基础上，对其部分基因片段进行了克隆及原核表达，并采用Western-blot对其免疫原性进行分析。结果显示，p208基因与猪肺炎支原体黏附素p216基因、殊异支原体细胞表面蛋白基因以及絮状支原体P97/LPPS家族蛋白基因具有较高的同源性。重组P208蛋白以可溶性形式在大肠杆菌BL21(DE3)工程菌中成功得到表达；纯化的重组蛋白与山羊抗绵羊肺炎支原体全菌血清发生结合反应，表明P208蛋白质是绵羊肺炎支原体的免疫原之一。研究结果为进一步分析其功能及其应用提供了重要的依据。
Abstract:
To investigate the structure and function of P208 protein of Mycoplasma ovipneumoniae , based on bioinformatics analysis, a fragment of p208 gene was amplified and expressed in Escherichia coil. The immunogenicity of P208 was analyzed by Western-blot. The results showed that p208 gene was homologous to the p216 in Mycoplasma hyopneumoniae, a gene encoding cell surface protein of Mycoplasma dispar and a gene encoding P97/LPPS family protein of Mycoplasma flocculare. The P208 was successfully expressed in E.coil BL21(DE3) with soluble form. The purified recombinant protein could react with goat serum against M. ovipneumoniae, this suggesting that P208 was one of the immunogens of M. ovipneumoniae. The results provide an important basis for further analysis of the function and application of P208.

参考文献/References:

[1]BESSER T E，HIGHLAND M A，KATHERINE B，et al. Causes of pneumonia epizootics among bighorn sheep，western United States[J]. Emerging Infectious Diseases，2012，18(3):406-414.
[2]RIFATBEGOVIC M，MAKSIMOVIC Z，HULAJ B. Mycoplasma ovipneumoniae associated with severe respiratory disease in goats[J]. Veterinary Record Journal of the British Veterinary Association，2011，168(21):565.
[3]胡新岗，黄银云，郭广富，等．利用PCR技术检测波杂肉山羊中的绵羊肺炎支原体［Ｊ］．江苏农业科学，2016，44（7）：271-273.
[4]GONALVES R，MARIANO I，NU＇EZ A，et al. Atypical non-progressive pneumonia in goats[J]. Veterinary Journal，2010，183(2):219-221.
[5]DASSANAYAKE R P，SHANTHALINGAM S，HERNDON C N，et al. Mycoplasma ovipneumoniae can predispose bighorn sheep to fatal Mannheimia haemolytica pneumonia[J]. Veterinary Microbiology，2010，145(3/4):354-359.
[6]GIANGASPERO M，NICHOLAS R A，HLUSEK M，et al. Seroepidemiological survey of sheep flocks from Northern Japan for Mycoplasma ovipneumoniae and Mycoplasma agalactiae[J]. Tropical Animal Health and Production，2012，44(3):395-398.
[7]ETTORRE C，SACCHINI F，SCACCHIA M，et al. Pneumonia of lambs in the Abruzzo region of Italy: anatomopathological and histopathological studies and localisation of Mycoplasma ovipneumoniae[J]. Veterinaria Italiana，2007，43(1):149-155.
[8]张萍慧，郝永清，张爱荣，等. 绵羊肺炎支原体的分离与鉴定[J]. 畜牧与饲料科学，2010，31(1):142-143.
[9]PETTERSSON B，UHLN M，JOHANSSON K E. Phylogeny of some mycoplasmas from ruminants based on 16S rRNA sequences and definition of a new cluster within the hominis group[J]. International Journal of Systematic Bacteriology，1996，46(4):1093-1098.
[10]LI M，MA C J，LIU X M，et al. Molecular cloning of HSP70 in Mycoplasma ovipneumoniae and comparison with that of other mycoplasmas[J]. Genetics and Molecular Research，2011，10(2):834-848.
[11]HSU T，MINION F C. Molecular analysis of the P97 cilium adhesin operon of Mycoplasma hyopneumoniae[J]. Gene，1998，214(2):13-23.
[12]WOOLLEY L K，FELL S A，GONSALVES J R，et al. Evaluation of recombinant Mycoplasma hyopneumoniae P97/P102 paralogs formulated with selected adjuvants as vaccines against mycoplasmal pneumonia in pigs[J]. Vaccine，2014，32(34):4333-4341.
[13]WILTON J，JENKINS C，CORDWELL S J，et al. Mhp493 (P216) is a proteolytically processed，cilium and heparin binding protein of Mycoplasma hyopneumoniae[J]. Molecular Microbiology，2009，71(3):566-582.
[14]杜海霞，刘茂军，冯志新，等. 猪肺炎支原体P216基因片段的表达及黏附活性研究[J]. 畜牧兽医学报，2012，43(8):1324-1329.
[15]FALONG Y，CHENG T，YONG W，et al. Genome sequence of Mycoplasma ovipneumoniae strain SC01[J]. Journal of Bacteriology，2011，193(18):5018.
[16]杨发龙，张贤宇，汤承，等. 绵羊肺炎支原体P113蛋白C端重复区的表达及其免疫原性[J]. 中国兽医科学，2013(7):733-737.
[17]张贤宇，杨发龙，冯旭飞，等. 绵羊肺炎支原体P113蛋白N端原核表达及其抗原性分析[J]. 中国兽医杂志，2013(7):7-10.
[18]谢珊珊，黄金，杨发龙. 绵羊肺炎支原体P109蛋白质分子特征、原核表达及其免疫反应性[J]. 江苏农业学报，2015，31(4):824-828.
[19]冯旭飞，刀筱芳，张贤宇，等. 绵羊肺炎支原体p128基因序列分析及原核表达[J]. 中国兽医杂志，2015，51(2):20-26.
[20]王娟，刀筱芳，杨发龙. 绵羊肺炎支原体P108蛋白的分子特征分析及免疫原性研究[J]. 中国兽医科学，2016，46(7):894-899.
[21]ADAMS C，PITZER J，MINION F C.In vivo expression analysis of the P97 and P102 paralog families of Mycoplasma hyopneumoniae[J]. Infection and Immunity，2005，73(11):7784-7787．

相似文献/References:

[1]谢珊珊,黄金,杨发龙.绵羊肺炎支原体P109蛋白质分子特征、原核表达及其免疫反应性[J].江苏农业学报,2015,(04):824.[doi:10.3969/j.issn.1000-4440.2015.04.018]
　XIE Shan-shan,HUANG Jin,YANG Fa-long.Molecular characterization, prokaryotic expression and immunoreactivity of Mycoplasma ovipneumoniae P109 protein[J].,2015,(01):824.[doi:10.3969/j.issn.1000-4440.2015.04.018]

备注/Memo

备注/Memo:
收稿日期：2017-06-23 基金项目：“十三五”国家重点研发计划项目(2016YFD0500907)；西南民族大学研究生创新型科研项目（CX2016SZ090） 作者简介：管礼麟（1994-），男，江西赣州人，硕士研究生，主要从事动物病原生物学的研究。（Tel）13072853553；（E-mail）1129979411@qq.com 通讯作者：杨发龙，（E-mail）yfalong@hotmail.com
更新日期/Last Update: 2018-03-06