[1]姜静,王银磊,赵丽萍,等.抗番茄黄化曲叶病基因ty-5序列变异及表达分析[J].江苏农业学报,2017,(05):1105-1110.[doi:doi:10.3969/j.issn.1000-4440.2017.05.022]
　JIANG Jing,WANG Yin-lei,ZHAO Li-ping,et al.Sequence variation and expression analysis of tomato yellow leaf curl virus resistance gene ty-5[J].,2017,(05):1105-1110.[doi:doi:10.3969/j.issn.1000-4440.2017.05.022]
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抗番茄黄化曲叶病基因ty-5序列变异及表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年05期
页码:
1105-1110
栏目:
园艺
出版日期:
2017-10-30

文章信息/Info

Title:
Sequence variation and expression analysis of tomato yellow leaf curl virus resistance gene ty-5
作者:
姜静1; 2; 王银磊1; 赵丽萍1; 周蓉1; 李亚茹1; 2; 余文贵1; 赵统敏1
（1.江苏省农业科学院蔬菜研究所，江苏南京210014；2.南京农业大学园艺学院，江苏南京210014）
Author(s):
JIANG Jing1; 2; WANG Yin-lei1; ZHAO Li-ping1; ZHOU Rong1; LI Ya-ru1; YU Wen-gui1; ZHAO Tong-min1
（1.Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China；2.College of Horticulture, Nanjing Agricultural University, Nanjing 210014, China)
关键词:
番茄黄化曲叶病毒; 抗番茄黄化曲叶病基因（ty-5）; 碱基突变; 表达分析; 基因型鉴定
Keywords:
tomato yellow leaf curl virus; ty-5; gene mutation; expression analysis; genotype identification
分类号:
S641.203.4
DOI:
doi:10.3969/j.issn.1000-4440.2017.05.022
文献标志码:
A
摘要:
番茄黄化曲叶病是番茄的主要病害之一，选育抗病品种是抵御该病害的重要途径。本研究对目前应用较少的抗番茄黄化曲叶病基因（ty-5）进行研究，对克隆片段进行序列分析，确定该基因在启动子区域和转录区域均存在碱基突变，并利用含有该基因的抗病材料AVTO1227对ty-5的表达进行分析，不同材料中ty-5及其等位基因Ty-5的表达量在接种番茄黄化曲叶病毒（TYLCV）前后均没有显著变化。本研究为下一步针对ty-5翻译区域内单核苷酸变异导致其功能变化的研究提供了依据，同时也为抗番茄黄化曲叶病育种提供了有效的连锁标记。
Abstract:
Tomato yellow leaf curl virus(TYLCV) disease is one of the important diseases of tomato. Breeding of resistant varieties was an important way to defense TYLCV disease. In this study, ty-5 gene which rarely reported was cloned and its fragment sequence was analyzed. Sequence mutation happened in the promoter region and the transcribed region of ty-5. Resistant material AVTO1227, which containing the ty-5 gene, was used for expression analyzing of ty-5. The results showed that the expression level of ty-5 and Ty-5 did not have significant change before and after TYLCV inoculation in different tomato materials. This study provided theoretical basis for the next step research of the single nucleotide in the transcription region of ty-5. In the meantime, linkage marker of ty-5 also provided an effective method for breeding.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2016-11-22 基金项目：国家自然科学青年基金项目（31401884）；江苏省自然科学青年基金项目（BK20140739）； 江苏省农业科学院基本科研业务专项［ZX（15)4022］ 作者简介：姜静（1989-），女，山东烟台人，硕士研究生，主要从事番茄遗传育种研究。（Tel）15895996723；（E-mail）496399220@qq.com。王银磊为共同第一作者。 通讯作者：赵统敏，（E-mail）tmzhaomail@163.com。余文贵，（E-mail）wenguiyu@ma
更新日期/Last Update: 2017-11-03