[1]娄丽娜,刘哲,许园园,等.萝卜与芜菁异源三倍体杂种的获得及鉴定[J].江苏农业学报,2017,(04):881-889.[doi:doi:10.3969/j.issn.1000-4440.2017.04.024]
　LOU Li-na,LIU Zhe,XU Yuan-yuan,et al.Production and identification of an allotriploid hybrid of radish (Raphanus sativus L.)× turnip (Brassica rapa L. spp. rapa)[J].,2017,(04):881-889.[doi:doi:10.3969/j.issn.1000-4440.2017.04.024]
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萝卜与芜菁异源三倍体杂种的获得及鉴定()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年04期
页码:
881-889
栏目:
园艺
出版日期:
2017-08-30

文章信息/Info

Title:
Production and identification of an allotriploid hybrid of radish (Raphanus sativus L.)× turnip (Brassica rapa L. spp. rapa)
作者:
娄丽娜; 刘哲; 许园园; 苏小俊
（江苏省农业科学院蔬菜研究所，江苏南京210014）
Author(s):
LOU Li-na; LIU Zhe; XU Yuan-yuan; SU Xiao-jun
(Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
萝卜; 芜菁; 属间杂交; 异源三倍体杂种
Keywords:
radish; turnip; intergeneric hybridization; allotriploid hybrid
分类号:
S631.1
DOI:
doi:10.3969/j.issn.1000-4440.2017.04.024
文献标志码:
A
摘要:
为了探讨萝卜和芜菁属间杂交的亲和性，以及为开展萝卜和芜菁远缘杂交育种提供参考，以7份不同类型的萝卜材料为母本，6份不同类型的芜菁材料为父本进行属间杂交，人工授粉过程中采用100 mg/L甘氨酸（Gly）、60 mg/L赤霉素（GA3）和5%氯化钠（NaCl）溶液涂抹柱头的方法，促进受精及子房和胚的发育，以涂抹清水为对照，然后用组织培养方法对杂种种子进行无菌苗培养。通过调查授粉后7 d、14 d及21 d的杂交结荚率及 7～21 d的结荚率差值，分析萝卜和芜菁属间杂交的亲和性；然后通过形态学和细胞学鉴定，确定杂种的真实性。结果表明：授粉后7 d，结荚率较高，随着授粉后时间的推移，结荚率迅速减少。不同杂交组合以及不同处理，对结荚率和结籽率的影响不同，组合R5×W4的结荚率保持最好，且结荚率最高；Gly处理对结荚率的保持效果较好，GA3处理获得种子的组合数量最多且结籽率最高。7个杂交组合获得74粒种子，出苗4株，经鉴定，在组合R9×W9上使用Gly处理，获得1株异源三倍体的属间杂种苗(3n=28)，杂种高度不育，未获得萝卜和芜菁的双单倍体杂种后代。
Abstract:
To analyze the crossability of radish and turnip, the distant hybridization between seven types of radish and six types of turnip materials were made in this study. Gly (100 mg/L), GA3 (60 mg/L), and NaCl (5%) solutions were spread on stigmas to accelerate fertilization and improve development of ovaries and ovules. Water was used as control. At 7 d, 14 d, and 21 d after pollination, the pod setting rate and its difference between 7 d and 21 d were investigated. The pod setting rate was high at 7 d after pollination, and decreased over time. The pod and seed setting rates varied in cross combinations and treatments. Cross combination R5×W4-2 showed the highest pod setting rate and the smallest pod setting rate drop. The Gly treatment had better effect on maintaining the pod setting rate. GA3-treated cross combinations got the highest number of seeds and the highest seed setting rate. Four seedlings emerged from 74 seeds of 7 crosses. One allotriploid hybrid (3n=28) was identified as highly sterile from Gly-treated combination R9×W9-1. No dihaploid hybrid was produced.

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备注/Memo

备注/Memo:
收稿日期：2017-03-27 基金项目：江苏省自然科学基金（青年基金）项目（BK20130726）；江苏省农业科技自主创新基金项目[CX（16）1012] 作者简介：娄丽娜（1982-），女，河南濮阳人，博士，副研究员，研究方向为蔬菜作物遗传育种。(Tel) 025-84391221; (E-mail) linabeibei@163.com 通讯作者：苏小俊，（E-mail）xiaojunsu@yahoo.com
更新日期/Last Update: 2017-09-01