[1]毕可然,韩凯凯,李银,等.樱桃谷鸭OASL基因全长cDNA的克隆及其生物信息学分析[J].江苏农业学报,2017,(02):373-378.[doi:doi:10.3969/j.issn.1000-4440.2017.02.021]
 BI Ke-ran,HAN Kai-kai,LI Yin,et al.Cloning and bioinformatics analysis of cherry valley duck oligoadenylate synthase-like gene(OASL)[J].,2017,(02):373-378.[doi:doi:10.3969/j.issn.1000-4440.2017.02.021]
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樱桃谷鸭OASL基因全长cDNA的克隆及其生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年02期
页码:
373-378
栏目:
畜牧兽医·水产养殖
出版日期:
2017-04-30

文章信息/Info

Title:
Cloning and bioinformatics analysis of cherry valley duck oligoadenylate synthase-like gene(OASL)
作者:
毕可然韩凯凯李银刘青涛赵冬敏刘宇卓黄欣梅杨婧
(江苏省农业科学院兽医研究所/农业部兽用生物制品工程技术重点实验室/国家兽用生物制品工程技术研究中心,江苏南京210014)
Author(s):
BI Ke-ranHAN Kai-kaiLI YinLIU Qing-taoZHAO Dong-minLIU Yu-zhuoHUANG Xin-meiYANG Jing
( Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bioproducts, Nanjing 210014, China)
关键词:
樱桃谷鸭OASL基因RACE生物信息学分析
Keywords:
cherry valley duckOASLRACEbioinformatics analysis
分类号:
S858.32
DOI:
doi:10.3969/j.issn.1000-4440.2017.02.021
文献标志码:
A
摘要:
为了获得樱桃谷鸭寡腺苷酸合成酶样蛋白质基因(OASL)全长cDNA,并且初步了解其生物学特性,采用RT-PCR和RACE方法从鸭脾脏组织总RNA中扩增OASL基因cDNA片段,应用生物信息学方法,系统分析鸭OASL的遗传进化以及蛋白质的理化性质、二级结构和亚细胞定位。结果表明,樱桃谷鸭OASL基因(GenBank登录号: KX255654)全长1 630 bp,其中包含19 bp的5′UTR(Untranslated region,UTR)、99 bp的3′UTR(Untranslated region, UTR)和PolyA尾巴、1 512 bp的编码区(Coding region,CDS), 翻译编码504个氨基酸多肽。鸭OASL蛋白具有寡腺苷酸合成酶(OAS)蛋白家族的典型特征,即N端为寡腺苷酸合成酶样结构域(OAS-like domain,OLD),C端为2个串联的泛素化样结构域(Ubiquitin-like domains, UbLDs),不具有信号肽和跨膜区。序列比对和系统进化分析结果表明,OASL氨基酸序列在同种之间比较保守,与已报道的鸭OASL的同源性高达100%,与鹅同源性为78%,与人、鼠和猪等哺乳动物的同源性仅为43%~45%。
Abstract:
To analyze the biological charateristics of oligoadenylate synthase-likely gene (OASL) in cherry valley duck, the complete cDNA of duck OASL gene was obtained by RT-PCR, 5′RACE and 3′RACE methods. The genetic evolutionary relationships, physical and chemical characteristics, second structure and subcellular localization of OASL were analyzed by bioinformatics tools. Results were as follows. The full-length cDNA sequence of OASL gene is 1 630 bp in size, including a 19-bp 5′ UTR, a 99-bp 3′UTR with the poly (A) tail (GenBank accession No. KX255654) and an open reading frame (ORF) of 1 512 bp in length, and encodes a predicted protein of 504 amino acids. OASL protein shared some typical characteristics of OAS family which is one OAS-like domain in the N-terminal and two tandem ubiquitin-like (UBL) domains in the C termined. No putative signal peptide and transmembrane domain were predicted. Sequence alignment and phylogenetic analysis showed that duck OASL was highly conserved among the same population, which shared 100% homology of aminoacid sequence with Anas platyrhynchos, 78% with Anser cygnoide.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-07-13 基金项目:国家自然科学基金项目( 31172345) ;江苏省自然科学基金项目(BK20160064);国家博士后基金项目(2016M590430) 作者简介:毕可然(1978-),女,内蒙古赤峰人,博士,讲师,主要从事水生生物病原学和遗传免疫学研究。(E-mail)bikeran@126.com 通讯作者:李银,(E-mail)muziyin08@126.com
更新日期/Last Update: 2017-05-02