[1]杜小姣,梁小红,葛永强,等.大叶落地生根谷氨酸脱羧酶基因(KdGAD)的克隆与表达[J].江苏农业学报,2017,(01):34-42.[doi:10.3969/j.issn.1000-4440.2017.01.006 ]
　DU Xiao-jiao,LIANG Xiao-hong,GE Yong-qiang,et al.cDNA cloning and expression analysis of glutamate decarboxylase gene (KdGAD) in Kalanchoe daigremontiana[J].,2017,(01):34-42.[doi:10.3969/j.issn.1000-4440.2017.01.006 ]
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大叶落地生根谷氨酸脱羧酶基因(KdGAD)的克隆与表达()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年01期
页码:
34-42
栏目:
遗传育种·生理生化
出版日期:
2017-02-28

文章信息/Info

Title:
cDNA cloning and expression analysis of glutamate decarboxylase gene (KdGAD) in Kalanchoe daigremontiana
作者:
杜小姣1; 梁小红2; 葛永强1; 段雪娇1; 张利娟3; 钟天秀4
（1.深圳市日昇园林绿化有限公司，广东深圳518040；2.北京林业大学林学院草坪研究所，北京100083；3.暨南大学深圳旅游学院，广东深圳518053；4.华南农业大学林学与风景园林学院草业科学系，广东省草业工程技术研究中心，广东广州510642）
Author(s):
DU Xiao-jiao1; LIANG Xiao-hong2; GE Yong-qiang1; DUAN Xue-jiao1; ZHANG Li-juan3; ZHONG Tian-xiu4
(1.Shenzhen Risheng Landscape Company Limited, Shenzhen 518040, China;2.Turfgrass Research Institute, Beijing Forestry University, Beijing 100083,China;3.Shenzhen Tourism College of Ji′nan University, Shenzhen 518053, China;4.College of Forestry and Landscape Architecture, South China Agricultural University, Guangdong Engineering Research Center of Grassland Science，Guangzhou 510642, China)
关键词:
大叶落地生根; 谷氨酸脱羧酶; 基因克隆; 基因表达
Keywords:
Kalanchoe daigremontiana; glutamate decarboxylase; gene cloning; gene expression
分类号:
S682.1+9
DOI:
10.3969/j.issn.1000-4440.2017.01.006
文献标志码:
A
摘要:
为研究大叶落地生根中胎生苗发育的分子机制，利用RACE-PCR技术从大叶落地生根中克隆了1个新的谷氨酸脱羧酶基因(KdGAD)。该基因开放阅读框长度为1 509 bp，编码502个氨基酸残基，分子量为 5.657×104，等电点为5.43。其氨基酸序列与蓖麻的同源性最高，与人参的进化关系最近，含有保守的Ser (S)-x-x-Lys (K)基序和Trp (W)残基。实时荧光定量PCR分析结果表明，该基因在大叶落地生根的茎中表达量最高，且受渗透胁迫(甘露醇处理)的诱导下调表达。
Abstract:
To better understand the molecular mechanisms of plantlet formation involved in Kalanchoe daigremontiana, a glutamate decarboxylase (GAD) gene, KdGAD, was identified using rapid amplification of cDNA end (RACE) PCR. KdGAD gene consisted of an ORF of 1 509 bp， which was predicted to encode a 502 amino acid residue protein of 5.657×104 with an isoelectric point of 5.43. The sequence analysis of the KdGAD revealed homology to Ricinus communis.Phylogenetic analysis showed that KdGAD protein was most related to Panax ginseng. In addition, KdGAD protein possessed a Ser (S)-X-X-Lys (K) active site and a single tryptophan (W) residue.Real-time PCR analysis revealed that KdGAD transcript was expressed highly in stem and down-regulated under osmotic stress (treatment with mannitol).

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相似文献/References:

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　GE Yong-qiang,ZHANG Li-juan,DU Xiao-jiao,et al.Cloning and expression analysis of histidinol dehydrogenase(KdHDH) gene in Kalanchoe daigremontiana[J].,2017,(01):280.[doi:doi:10.3969/j.issn.1000-4440.2017.02.007]

备注/Memo

备注/Memo:
收稿日期：2016-05-17 基金项目：深圳市知识创新项目（20160093）；北京林业大学与深圳市日昇园林绿化有限公司产学研合作项目 作者简介：杜小姣（1987-），女，河南邓州人，本科，工程师，主要从事园林绿化工作。（Tel）15889600573；（E-mail）15889600573@163.com 通讯作者：钟天秀，（E-mail）zhongxinbi@163.com
更新日期/Last Update: 2017-04-12