[1]范志宇,魏后军,仇汝龙,等.一株新型家兔肠炎沙门氏菌的分离鉴定及防治[J].江苏农业学报,2016,(06):1367-1371.[doi:doi:10.3969/j.issn.1000-4440.2016.06.026]
　FAN Zhi-yu,WEI Hou-jun,QIU Ru-long,et al.Isolation, identification and control of a novel Salmonella enteritidis strain from rabbit[J].,2016,(06):1367-1371.[doi:doi:10.3969/j.issn.1000-4440.2016.06.026]
点击复制

一株新型家兔肠炎沙门氏菌的分离鉴定及防治()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年06期
页码:
1367-1371
栏目:
畜牧兽医·水产养殖
出版日期:
2017-02-07

文章信息/Info

Title:
Isolation, identification and control of a novel Salmonella enteritidis strain from rabbit
作者:
范志宇; 魏后军; 仇汝龙; 胡波; 宋艳华; 陈萌萌; 徐为中; 王辉; 薛家宾;  王芳
(江苏省农业科学院兽医研究所/农业部动物疫病诊断与免疫重点开放实验室/国家兽用生物制品工程技术研究中心，江苏南京210014)
Author(s):
FAN Zhi-yu; WEI Hou-jun; QIU Ru-long; HU Bo; SONG Yan-hua; CHEN Meng-meng; XU Wei-zhong; WANG Hui; XUE Jia-bin; WANG Fang
(Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biologicals Engineering and Technology，Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014,China)
关键词:
兔; 肠炎沙门氏菌; 16S rRNA基因扩增技术; 特异性PCR
Keywords:
rabbit; Salmonella enteritidis; 16S rRNA gene amplification; specific PCR
分类号:
S829.17
DOI:
doi:10.3969/j.issn.1000-4440.2016.06.026
文献标志码:
A
摘要:
针对山东省某兔场发生以仔兔脾脏肿大、母兔流产为特征的传染病，本研究对发病濒死兔进行细菌分离，利用16S rRNA基因扩增技术、特异性PCR技术和生化试验对致病细菌进行鉴定，并进行了药敏试验和自制疫苗效力试验。分离菌株16S rRNA基因序列与肠炎沙门氏菌相似度大于99.0%，培养特性和生化特性符合肠炎沙门氏菌特性。分离菌株特异性PCR产物序列与肠炎沙门氏菌sefA基因的同源性为99.3%～100.0%，因此将其命名为Salmonella SD/2016。动物回归试验成功复制出该病并分离到目的细菌。自制疫苗安全性良好，对断奶幼兔能提供免疫保护。
Abstract:
In February 2016, a novel contagious disease outbroke in a rabbit farm of Shandong province, characterized by splenomegaly of weaning rabbit and abortion of female rabbit. The pathogens bacteria were isolated and identified by biochemical tests, specific PCR assay, 16S rRNA detection and antimicrobial susceptibility test.The safety and efficiency of the autogenous vaccine were detected as well. The 16S rRNA gene sequences of the isolates shared homologies of 99.0% with that of Salmonella enteritidis, and the isolates presented the cultural and chemical characteristic of S. enteritidis. The sefA gene of the isolates shared 99.3% to 100.0% homology with that of S. enteritidis. Therefore, the isolate was named Salmonella SD/2016 strain. A similar disease was reproduced in healthy rabbits and mice, and the bacteria were successfully isolated. Self-made vaccine was safe and could provide immune protection to weaning rabbit.

参考文献/References:

[1]殷俊磊，李求春，焦新安. 沙门菌致病岛2Ⅲ型分泌系统研究进展[J].微生物学报，2016，56(4)：561-569.
[2]HUR J，JAWALE C，LEE J H，et al. Antimicrobial resistance of Salmonella isolated from food animals: A review[J]. Food Research International，2012，45（2）：819-830.
[3]尹德凤，张莉，张大文，等. 食品中沙门氏菌污染研究现状[J]. 江西农业学报，2015，27( 11)：55-60.
[4]蒋颖，刘轶，郦晓琼，等.肠炎沙门氏菌特异性诊断方法的建立[J]. 扬州大学学报(农业与生命科学版)，2007，28(3)：6-8.
[5]CORBY-HARRIS V，PONTAROLI A C，SHIMKETS L J，et al. Geographical distribution and diversity of bacteria associated with natural populations of Drosophila melanogaster[J]. Applied and Environmental Microbiology, 2007，73(11)：3470-3479.
[6]陆承平.兽医微生物学[M].3版.北京：中国农业出版社，2007：223-231.
[7]中国兽药典委员会．中华人民共和国兽药典：三部[M].北京：中国农业出版社，2011：附录42-44.
[8]范志宇，魏后军，胡波，等.兔出血症病毒杆状病毒载体灭活疫苗安全性及效力试验[J].江苏农业科学，2015，43(11)：272-275.
[9]中华人民共和国卫生部.食品微生物学检验—沙门菌检验：GB/T4789.4-2010[S].
[10]谷子林，秦应和，任克良.中国养兔学[M].北京：中国农业出版社，2013：522-523.
[11]STEENACKERS H，HERMANS K，VANDERLEYDEN J，et al. Salmonella biolms: An overview on occurrence，structure，regulation and eradication[J].Food Research International，2012，45(2)： 502-531.
[12]JAJERE S M， ADAMU N B， ATSANDA N N，et al. Prevalence and antimicrobial resistance profiles of Salmonella isolates in apparently healthy slaughtered food animals at Maiduguri central abattoir，Nigeria [J].Asian Pacific Journal of Tropical Disease，2015，5(12)：996-1000.

相似文献/References:

[1]潘孝青,王杏龙,杨杰,等.不同LED光色对家兔繁殖性能的影响及其分子调控机制[J].江苏农业学报,2020,(01):136.[doi:doi:10.3969/j.issn.1000-4440.2020.01.019]
　PAN Xiao-qing,WANG Xing-long,YANG Jie,et al.Effects of different LED light colors on reproductive performance of rabbits and their molecular regulation mechanism[J].,2020,(06):136.[doi:doi:10.3969/j.issn.1000-4440.2020.01.019]

备注/Memo

备注/Memo:
收稿日期：2016-08-29 基金项目：现代农业产业技术体系建设兔体系病毒病预防与控制岗位项目（CARS-44-C-1）；农业部公益性行业（农业）科研专项项目（201303046） 作者简介：范志宇(1982-)，男，山西太谷人，博士，助理研究员，主要从事家兔疾病防治与兽医生物技术研究。（Tel）025-84390337；（E-mail）fanzhiyu2007@163.com 通讯作者：王芳，（E-mail）rwangfang@126.com
更新日期/Last Update: 2017-02-07