[1]姜 骁,吴拥军,杨 亚,等.烟草钾外流通道基因NTORK1的启动子克隆及激素诱导分析[J].江苏农业学报,2016,(02):274-277.[doi:10.3969/j.issn.1000-4440.2016.02.006]
 JIANG Xiao,WU Yong-jun,YANG Ya,et al.Cloning and phytohormone induction of the promotor of potassium outward channel gene NTORK1 from tobacco[J].,2016,(02):274-277.[doi:10.3969/j.issn.1000-4440.2016.02.006]
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烟草钾外流通道基因NTORK1的启动子克隆及激素诱导分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年02期
页码:
274-277
栏目:
遗传育种·生理生化
出版日期:
2016-03-20

文章信息/Info

Title:
Cloning and phytohormone induction of the promotor of potassium outward channel gene NTORK1 from tobacco
作者:
姜 骁1 吴拥军1 杨 亚1 赵杰宏2
1.贵州大学生命科学学院/贵州省农业生物工程重点实验室,贵州 贵阳 550025; 2.贵州省烟草科学研究院/烟草行业分子遗传重点实验室,贵州 贵阳 550081
Author(s):
JIANG Xiao1 WU Yong-jun1 YANG Ya1 ZHAO Jie-hong2
1.College of Life Science, Guizhou University/Guizhou Key Laboratory of Agricultural Bioengineering, Guiyang 550025, China; 2.Guizhou Academy of Tobacco Science/Key Laboratory of Molecular Genetics of CNTC, Guiyang 550081, China
关键词:
烟草 钾外流通道基因NTORK1 启动子 激素
Keywords:
tobacco potassium outward channel gene NTORK1 promoter phytohormone
分类号:
S572
DOI:
10.3969/j.issn.1000-4440.2016.02.006
文献标志码:
A
摘要:
为研究烟草钾外流通道基因NTORK1的启动子的表达特征,以烟草K326基因组DNA为模板,PCR扩增获得NTORK1的启动子片段,该启动子片段为2 111 bp,包含多个转录因子结合域。构建了NTORK1启动子驱动的GUS基因表达载体,在烟草K326叶片中进行瞬时表达分析。结果显示,NTORK1启动子受低浓度NAA(5.4 μmol/L)和高浓度SA(500 μmol/L)的诱导表达。
Abstract:
To characterize the expression of the promotor of potassium outword channel gene NTORK1, a upstream fragment of NTORK1 was amplified from Nicotiana tabacum K326 genome, containing 2 011 nucleotides and multiple transcription factor binding domain. The plant expression vector pBI121-NTORK1 was introduced into K326 leaf for transient expression. The promotor of NTORK1 was expressed under the induction of hormones NAA(5.4 μmol/L)and SA(500 μmol/L).

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-11-03 基金项目:中国烟草总公司科技重大专项[110201401007(JY-07)]; 中国烟草总公司科技重大专项(Ts-02-20110013); 贵州省农业攻关科技计划项目[黔科合NY(2013)3020号] 作者简介:姜 骁(1990-),男,山东青岛人,硕士研究生,研究方向为植物分子生物学与基因工程。(E-mail)15066223517@163.com 通讯作者:赵杰宏,(E-mail)zhaojiehong@126.com
更新日期/Last Update: 2016-03-20