[1]金崇富,陈长宽,杨智青,等.鸡IGF1R、IGFBP-3基因SNP位点之间互作效应分析[J].江苏农业学报,2015,(05):1099-1104.[doi:doi:10.3969/j.issn.1000-4440.2015.05.024]
　JIN Chong-fu,CHEN Chang-kuan,YANG Zhi-qing,et al.Genetic interaction effect between SNPs loci of IGF1R and IGFBP-3 genes in chicken[J].,2015,(05):1099-1104.[doi:doi:10.3969/j.issn.1000-4440.2015.05.024]
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鸡IGF1R、IGFBP-3基因SNP位点之间互作效应分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
1099-1104
栏目:
畜牧兽医·水产养殖
出版日期:
2015-10-31

文章信息/Info

Title:
Genetic interaction effect between SNPs loci of IGF1R and IGFBP-3 genes in chicken
作者:
金崇富; 陈长宽; 杨智青; 时凯; 陈应江
(江苏沿海地区农业科学研究所，江苏盐城224002)
Author(s):
JIN Chong-fu; CHEN Chang-kuan; YANG Zhi-qing; SHI Kai; CHEN Ying-jiang
(Institute of Agricultural Sciences in the Coastal District of Jiangsu Province, Yancheng 224002, China)
关键词:
鸡; 单核苷酸多态性; IGF1R基因; IGFBP-3基因; 互作效应
Keywords:
chicken; single nucleotide polymorphism (SNP); IGF1R gene; IGFBP-3 gene; interaction effect
分类号:
S831.2
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.024
文献标志码:
A
摘要:
以IGF1R、IGFBP-3基因作为影响鸡生产性能的候选基因，采用DNA测序和PCR-SSCP技术分析了单核苷酸多态性（SNP），并对两基因SNP位点之间的互作效应进行相关分析。结果表明，在IGF1R和IGFBP-3基因中分别发现2个SNP位点（IGF1R基因的G26333A和G26336A，IGFBP-3基因的T160G和C1087T），其中IGF1R基因G26336A位点对鸡的生长性状具有显著的影响（P<0.05）；G26336A位点DD基因型个体8周龄体质量显著高于CC基因型（P<0.05），DD基因型个体12周龄体质量显著高于CC和CD基因型（P<0.05）；IGFBP-3基因C1087T位点GH基因型个体的开产日龄显著低于HH基因型（P<0.05）。IGF1R基因G26336A位点与IGFBP-3基因C1087T位点之间对12周龄体质量有互作效应。可见，IGF1R和IGFBP-3基因可能是影响鸡生产性状的主效基因。
Abstract:
In this research, IGF1R and IGFBP-3 genes were selected as candidate genes affecting production performance of chicken. PCR-SSCP and DNA sequencing were employed to detect the single nucleotide polymorphisms (SNPs) so as to analyze the genetic interaction effect between SNPs loci. Two mutations (G26333A and G26336A) of IGF1R gene and two mutations (T160G and C1087T) of IGFBP-3 gene were found in chicken. G26336A in the exonic region of IGF1R gene affected growth traits （P<0.05）. For locus G26336A, chickens with genotype DD showed heavier body weight than those with genotype CC at 8-week age （P<0.05） and than those with genotypes CC and CD at 12-week age （P<0.05）. For C1087T in the exonic region of IGFBP-3 gene, chickens with genotype GH had lower age at first egg than those with genotype HH (P<0.05). An interactive effect was detected between mutations G26336A and C1087T on chick body weight at 12-week age. These results suggest that IGF1R and IGFBP-3 could be the major genes responsible for productive performance in chicken.

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备注/Memo

备注/Memo:
收稿日期：2015-02-27 基金项目：江苏省农业科技自主创新基金项目[CX(13)3044]；国家级星火计划项目（2013GA690334） 作者简介：金崇富（1985-），男，江苏建湖人，硕士，助理研究员，从事动物遗传育种与繁殖研究。(Tel)15189313501；(E-mail)jincf001@sina.com 通讯作者：陈应江，(Tel)13705102062；(E-mail)cyj-1111@163.com
更新日期/Last Update: 2015-10-31