[1]齐中强,杜艳,刘永锋.Rho型GTPase激活蛋白MoBem2在稻瘟病菌分生孢子形态建成中的功能[J].江苏农业学报,2015,(05):1001-1005.[doi:doi:10.3969/j.issn.1000-4440.2015.05.009]
　QI Zhong-qiang,DU Yan,LIU Yong-feng.Role of Rho GTPase-activated protein MoBem2 in conidium morphogenesis of Magnaporthe oryzae[J].,2015,(05):1001-1005.[doi:doi:10.3969/j.issn.1000-4440.2015.05.009]
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Rho型GTPase激活蛋白MoBem2在稻瘟病菌分生孢子形态建成中的功能()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
1001-1005
栏目:
植物保护
出版日期:
2015-10-31

文章信息/Info

Title:
Role of Rho GTPase-activated protein MoBem2 in conidium morphogenesis of Magnaporthe oryzae
作者:
齐中强; 杜艳; 刘永锋
（江苏省农业科学院植物保护研究所，江苏南京210014）
Author(s):
QI Zhong-qiang; DU Yan; LIU Yong-feng
(Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
稻瘟病菌; MoBem2; 分生孢子
Keywords:
Magnaporthe oryzae; MoBem2; conidium
分类号:
S435.111.4+1
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.009
文献标志码:
A
摘要:
为了研究Rho型GTPase激活蛋白编码基因MoBEM2（MGG_09303）在稻瘟病菌致病过程中的作用，利用基因敲除方法进行了缺失突变。结果显示该基因缺失突变体产孢量显著下降40%左右，利用荧光增白剂 (CFW)染色发现孢子形状变得畸形，形成单个隔膜孢子，约为40%左右，而野生型超过90%均为正常孢子；另外，该基因的缺失导致突变体在培养8 h时附着胞形成率显著下降，随着时间的延长，突变体附着胞形成率逐渐上升到野生型程度。可见，MoBem2蛋白调控稻瘟病菌分生孢子的形态建成，延缓分生孢子萌发过程中附着胞的形成，但对稻瘟病菌的致病性没有影响。
Abstract:
To study the function of Rho GTPase activated protein coding gene MoBEM2 (MGG_09303) in conidium morphogenesis, the deletion mutant was identified using the knock-out method in rice blast fungus Magnaporthe oryzae. The deletion mutant produced less conidia (reduced by 40%), and staining by calcofluor white (CFW) showed that fourty percent of the conidia were uniseptate in contrast to ninety percent normal three-celled conidia of wild type. The appressorium formation was slowed down significantly at 8 h, but was restored over time. It is indicated MoBem2 is involved in conidium morphogenesis and appressorium formation.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2015-07-20 基金项目：国家自然科学基金青年基金项目（31401697）；江苏省农业科技自主创新基金项目[CX(15)1054] 作者简介：齐中强（1985-），男，河南驻马店人，博士，助理研究员，主要从事稻瘟病菌功能基因研究。（Tel）025-84391810；（E-mail）qizhongqiang2015@jaas.ac.cn 通讯作者：刘永锋，（Tel）025-84390180；（E-mail）liuyf@jaas.ac.cn
更新日期/Last Update: 2015-10-31