[1]常丽娟,宋君,雷绍荣,等.转基因玉米MIR604结构特异片段实时荧光定量检测方法的建立[J].江苏农业学报,2015,(05):971-974.[doi:doi:10.3969/j.issn.1000-4440.2015.05.005]
　CHANG Li-juan,SONG Jun,LEI Shao-rong,et al.A construct-specific real-time PCR for quantitative detection of genetically modified maize MIR604[J].,2015,(05):971-974.[doi:doi:10.3969/j.issn.1000-4440.2015.05.005]
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转基因玉米MIR604结构特异片段实时荧光定量检测方法的建立()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
971-974
栏目:
遗传育种·生理生化
出版日期:
2015-10-31

文章信息/Info

Title:
A construct-specific real-time PCR for quantitative detection of genetically modified maize MIR604
作者:
常丽娟; 宋君; 雷绍荣; 尹全; 王东; 刘文娟; 张富丽
（四川省农业科学院分析测试中心，四川成都610066）
Author(s):
CHANG Li-juan; SONG Jun; LEI Shao-rong; YIN Quan; WANG Dong; LIU Wen-juan; ZHANG Fu-li
(Analysis and Test Center, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China)
关键词:
转基因玉米; 结构特异性; 实时荧光定量PCR; 灵敏度; 准确度
Keywords:
genetically modified maize MIR604; structure-specificity; sensitivity; accuracy
分类号:
S634.3
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.005
文献标志码:
A
摘要:
根据转基因玉米MIR604外源载体序列，设计引物和探针，并优化反应体系和热循环条件，建立了MIR604结构特异片断实时荧光定量PCR检测方法。采用该方法对6种非转基因作物、MIR604及其他非目标转基因作物进行检测，除MIR604外，其余样品均未检测到MIR604分子片段的扩增信号；对含量为1%的MIR604标准品进行定量检测，测试样品平均含量为1.05%；灵敏度测试结果显示该方法能检测到仅5个拷贝的MIR604分子片段。因此，本研究建立的方法具有较高的特异性、准确度及灵敏度，能为中国转基因生物安全监管提供技术支持。
Abstract:
A structure-specific quantitative PCR technique for the detection of genetically modified maize MIR604 was developed in this study to provide supports for genetically modified organism safety supervision in China by optimizing reaction system and thermal cycling condition. Six kinds of non-genetically modified crops, MIR604 and other non-target transgenic crops were detected. The fluorescent signal was only detected in MIR604. The measured value of 1％ MIR604 was 1.05%. The sensitivity experiment revealed the method could detect only 5 copies of MIR604 molecular fragments. In conclusion, the structure-specific quantitative PCR detection method for genetically modified maize MIR604 is of high specificity, accuracy and sensitivity.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2015-01-15 基金项目：四川省质监局标准化技术体系项目（ZYBZ2013-39） 作者简介：常丽娟（1982-），女，四川成都人，硕士，助理研究员，从事转基因生物安全监管研究。（Tel）18908186732；（E-mail） juanjuanclj@126.com 通讯作者：宋君，（Tel）028-84504557；（E-mail）drsjn@126.com
更新日期/Last Update: 2015-10-31