[1]郑佳,丁丹,梁彦丽,等.极端粒形水稻品种GS5基因的序列及效应分析[J].江苏农业学报,2015,(05):951-956.[doi:doi:10.3969/j.issn.1000-4440.2015.05.001]
　ZHENG Jia,DING Dan,LIANG Yan-li,et al.Sequence and effect of extreme grain shape gene GS5 of rice (Oryza sativa L.)[J].,2015,(05):951-956.[doi:doi:10.3969/j.issn.1000-4440.2015.05.001]
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极端粒形水稻品种GS5基因的序列及效应分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
951-956
栏目:
遗传育种·生理生化
出版日期:
2015-10-31

文章信息/Info

Title:
Sequence and effect of extreme grain shape gene GS5 of rice (Oryza sativa L.)
作者:
郑佳1; 丁丹12; 梁彦丽12; 张亚东1; 王才林1
（1.江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心，江苏南京210014；2.南京农业大学农学院，江苏南京210095）
Author(s):
ZHENG Jia1; DING Dan12; LIANG Yan-li12; ZHANG Ya-dong1; WANG Cai-lin1
（1.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R&D Center/Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China;2.College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China)
关键词:
水稻; GS5; 克隆; 简单重复序列（SSR）
Keywords:
rice;  GS5; cloning; simple sequence repeat (SSR)
分类号:
S511；Q785
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.001
文献标志码:
A
摘要:
为明确粒型控制基因GS5在2种极端粒型水稻中的序列差异和作用，本研究利用极端大粒型水稻品种TD70和小粒型水稻品种Kasalath对粒宽控制基因GS5进行了克隆和序列分析，根据序列比对结果设计分子标记，用来区分不同来源的GS5基因，并结合重组自交系的粒型信息分析该基因对粒型的调节作用。结果显示,该基因包含10个外显子和9个内含子，与TD70的GS5基因相比，Kasalath的GS5基因第1外显子上编码的第31位氨基酸由甘氨酸变成丙氨酸，同时在第36、37位缺失2个甘氨酸。以这2个氨基酸的缺失为基础，设计简单重复序列（SSR）分子标记并在2个品种及其重组自交系（RIL）中进行了验证。利用该标记可以在含有GS5-T的品种中扩增出216 bp的片段，在含有GS5-K的品种中扩增出210 bp的片段。对重组自交系的检测结果显示，含有GS5-T基因的株系有122个，含有GS5-K基因的株系有118个。含GS5-T基因的株系平均粒宽比含GS5-K基因的株系平均粒宽高出0.47 mm，千粒质量高出2.87 g，说明GS5是水稻粒宽性状的主效调控基因。
Abstract:
In order to identify the differences of sequences and role of extreme grain gene GS5 in rice, TD70 with extremely large grain and Kasalath with extremely small grain were used to clone GS5 genes. According to the sequences differences, molecular markers were designed and the role of GS5 were analyzed through based on the grain shape of RIL. GS5 contained ten exons and nine introns. Compared with GS5 of TD70, GS5 of Kasalath showed a mutation changing the amino acid from Gly to Ala in +31 and deleted two glys in +36 and +37 at the first exon. Based on this deletion, a simple sequence repeat (SSR) molecular marker was designed and verified in the recombinant inbred lines (RILs). The marker could amplify a 216-bp fragment in rice with GS5-T and a 210-bp fragment in rice with GS5-K. In the 240 RILs, GS5-T genotype were 122 and GS5-k genotype were 118. The gain width of lines with GS5-T was 0.47 mm wider than those of lines with GS5-K，and the 1 000-grain weight (kgw)of lines with GS5-T was 2.87 g heavier than those of lines with GS5-K. These indicated that GS5 was the major gene controlling grain width.

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备注/Memo

备注/Memo:
收稿日期：2014-12-26基金项目：国家自然科学基金项目（31271678）;江苏省农业科技自主创新基金项目[CX(11)4023]；江苏省博士后基金项目(1302161C)作者简介：郑佳（1984-），女，安徽淮南人，博士后，研究方向为作物遗传育种。（Tel）13405852826;(E-mail) zhengjiajob@163.com通讯作者：王才林，(E-mail) clwang@jaas.ac.cn
更新日期/Last Update: 2015-10-31