[1]李永丰,张自常,杨霞,等.稻田稗属杂草对芳氧苯氧丙酸酯类除草剂的差异敏感性及其机理[J].江苏农业学报,2015,(03):543-551.[doi:10.3969/j.issn.1000-4440.2015.03.013]
　LI Yong-feng,ZHANG Zi-chang,YANG Xia,et al.Susceptibility of weeds in Echinochloa to aryloxyphenoxypropionate herbicides and the mechanism[J].,2015,(03):543-551.[doi:10.3969/j.issn.1000-4440.2015.03.013]
点击复制

稻田稗属杂草对芳氧苯氧丙酸酯类除草剂的差异敏感性及其机理()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年03期
页码:
543-551
栏目:
植物保护
出版日期:
2015-06-30

文章信息/Info

Title:
Susceptibility of weeds in Echinochloa to aryloxyphenoxypropionate herbicides and the mechanism
作者:
李永丰1; 张自常1; 杨霞1; 董明超1; 张彬1; 韩建勇2
(1.江苏省农业科学院植物保护研究所，江苏南京210014;2.美国FMC公司，江苏淮安223001)
Author(s):
LI Yong-feng1; ZHANG Zi-chang1; YANG Xia1; DONG Ming-chao1; ZHANG Bin1; HAN Jian-yong2
(1.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China；2.American FMC Corporation, Huaian 223001, China)
关键词:
稗草; 芳氧苯氧丙酸酯类除草剂; 噁唑酰草胺; 氰氟草酯; 抗氧化酶活性; MDA含量
Keywords:
Echinochloa; aryloxyphenoxypropionate herbicide; metamifop; cyhalofopbutyl; antioxidant enzyme activity; MDA content
分类号:
S451.21
DOI:
10.3969/j.issn.1000-4440.2015.03.013
文献标志码:
A
摘要:
采用整株生物测定法研究了8个稗草种对芳氧苯氧丙酸酯类除草剂噁唑酰草胺和氰氟草酯的敏感性，并分析了其生理基础。基于药后25 d干质量GR50（抑制杂草干质量50%所需药剂剂量）的聚类结果显示：对噁唑酰草胺来说，稗草为敏感种群，无芒稗为不敏感种群；对氰氟草酯来说，西来稗为敏感种群，而无芒稗为不敏感种群。稗属不同种群间对2种芳氧苯氧甲酸类除草剂存在明显的差异敏感性。从保护酶活性分析，随着噁唑酰草胺或氰氟草酯处理剂量的增加和时间的延长，无芒稗、西来稗和稗茎叶中的丙二醛（MDA）含量增加，相同剂量下敏感种群的丙二醛含量高于不敏感种群。2种除草剂处理后，过氧化物酶（POD）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性随剂量的增加呈先上升后下降的趋势，不敏感种群上升或下降幅度均低于敏感种群，至处理结束时，不敏感种群的酶活总体高于敏感种群。2种除草剂处理后不敏感稗草种群膜脂过氧化酶活性变化幅度小、活性高，丙二醛含量低，可能是其对芳氧苯氧丙酸酯类除草剂耐药的原因。
Abstract:
Echinochloa (barnyardgrass) has long been considered as one of the most troublesome weeds, causing significant yield loss due to its superiority to rice in competition. The Echinochloa could be classified to eight geographic species in Jiangsu province of China. To date, however, limited information is available on susceptibility of eight Echinochloa species to aryloxyphenoxypropionate herbicides. Therefore, the aim of this study was to investigate the susceptibility differences of eight Echinochloa species to aryloxyphenoxypropionate herbicides using whole-plant bioassay, and their physiological bases was also explored. The results, based on clustering analysis of GR50 in dry matter after 25-day treatment, showed that E. crusgalli (L.) Beavu, and E. crusgalli Beauv var. zelayensis (H.B.K) Hitche were respectively susceptible to metamifop and cyhalofopbutyl, while E. crusgalli Beauv var. zelayensis (H.B.K) Schult was tolerant to both herbicides. The susceptible difference also existed in different populations of Echinochloa to two types of aryloxyphenoxypropionate herbicides. The analyses of the protective enzyme activities revealed that the content of malondialdehyde (MDA) increased in the stem and leaves of E. cruspavonis (H.B.K) Schult, E. crusgalli (L.) Beauv var. zelayensis (H.B.K) Hitche and E. crusgalli (L.) Beavu with the increased doses of aryloxyphenoxypropionate herbicides over time of the application of metamifop or cyhalofopbutyl, and the content of MDA in susceptible population was higher than that in tolerant population in the same dose treatment. The activities of peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) ascended at first and descended afterwards after two herbicides treatment. The increases in the protective enzymes activities of susceptible populations were lower than that in tolerant one. At the end of the treatment, three kinds of enzymes activities in the susceptible population were lower than that in tolerant one. The results indicated that the slight change and higher activities in membrane lipid peroxidase and lower content of MDA in aryloxyphenoxypropionate herbicides-tolerant population of Echinochloa might be responsible for the tolerance.

参考文献/References:


[1]马凤霞，赵权，姜辉．3种植物提取液对稗草生长发育的影响[J]．江苏农业科学，2014，42(10)：127-129.
[2]张自常，李永丰，张彬，等．江苏省稻田常见稗草的生物学特性[J]．江苏农业科学，2013，41(12)：136-138.
[3]李海波，侯守贵，代贵金，等．水稻和稗草种间竞争关系研究现状[J]．中国农学通报，2011，27(15)：259-262.
[4]杨小育．世界性恶性杂草的危害[J]．世界农业，1992(4)：40-42.
[5]吴声敢，王强，赵学平，等．稻田稗草生物学特性及其综合防除[J]．杂草科学，2006(3)：1-6.
[6]张朝贤，倪汉文，魏守辉，等．杂草抗药性研究进展[J]．中国农业科学，2009，42(4)：1274-1289.
[7]SALIN M L. Toxic oxygen species and protective systems of the chloroplast[J]. Physiologia Plantarum, 1988, 72: 681-689.
[8]YANG C, DONG L, LI J, et al. Identification of Japanese foxtail (Alopecurus japonicus) resistant to haloxyfop using three different assay techniques[J]. Weed Science, 2007, 55: 537-540.
[9]赵世杰，许长成，邹琦，等．植物组织中丙二醛测定方法的改进[J]．植物生理学通讯，1994，30(3)：207-210.
[10]王学奎．植物生理生化试验原理和技术[M]．北京：高等教育出版社，2006：167-170.
[11]BERRY J, BJORKMAN O. Photosynthetic response and adaptation to temperature in higher plants[J]. Annual Review of Plant Physiology, 1980, 31: 491-543.
[12]乔丽雅．江苏省稻田稗属杂草生物学特性及其对扫氟特和乙草胺耐药性的研究[D]．南京：南京农业大学. 2003.
[13]LIANG Y C, HU F, YANG M C, et al. Antioxidative defenses and water deficit-induced oxidative damage in rice (Oryza sativa L.) growing non-flooded paddy soils with ground mulching[J].Plant and Soil, 2003,257:407-416.
[14]LIANG Y C, CHEN Q, LIU Q, et al. Exogenous silicon (Si) increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.)[J]. Journal of Physiology, 2003, 160: 1157-1164.
[15]路文静．植物生理学[M]．北京：中国林业出版社，2011.
[16]SHAALTIEL Y, GRESSEL J. Multienzyme oxygen radical detoxifying system correlate with paraquat resistance in Conyza bonariensis[J]. Pesticide Biochemistry Physiology, 1986, 26: 22-28.
[17]TURESANYI E, DARKO E, BORBELY G, et al. The activity of oxyradical-detoxifying enzymes is not correlated with paraquat resistance in Conyza Canadensis (L.)[J]. Pesticide Biochemistry Physiology, 1998, 60: 1-11.〖ZK）〗〖FL）〗

相似文献/References:

[1]杨霞,王笑,管荣展,等.多抗性稗草中 ２ 个谷胱甘肽转移酶基因的克隆与分析[J].江苏农业学报,2015,(06):1296.[doi:doi:10.3969/j.issn.1000-4440.2015.06.016]
　YANG Xia,WANG Xiao,GUAN Rong-zhan,et al.Cloning and characterization of two glutathione S-transferases genes from herbicide-resistant Echinochloa crusgalli[J].,2015,(03):1296.[doi:doi:10.3969/j.issn.1000-4440.2015.06.016]

备注/Memo

备注/Memo:
收稿日期：2014-11-17 基金项目：江苏省农业科技自主创新基金项目[CX（13）3063]；公益性行业（农业）科研专项项目（201303031） 作者简介：李永丰（1965-），男，江西寻乌人，硕士，研究员，主要从事作物田间恶性杂草的成灾机制与防控研究。(Tel)025-84390821；(E-mail) liyongfeng_2010@163.com〖ＨＴ〗〖ＦＱ）〗
更新日期/Last Update: 2015-06-30