[1]国政,李婉婉,杨晗,等.月季内生放线菌GZ-57的抑菌活性成分[J].江苏农业学报,2015,(02):304-309.[doi:10.3969/j.issn.1000-4440.2015.02.013]
　GUO Zheng,LI Wan-wan,YANG Han,et al.Antimicrobial ingredients of GZ-57, an endophytic actinomycete from Rosa chinensis Jacq.[J].,2015,(02):304-309.[doi:10.3969/j.issn.1000-4440.2015.02.013]
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月季内生放线菌GZ-57的抑菌活性成分()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
304-309
栏目:
植物保护
出版日期:
2015-04-30

文章信息/Info

Title:
Antimicrobial ingredients of GZ-57, an endophytic actinomycete from Rosa chinensis Jacq.
作者:
国政; 李婉婉; 杨晗; 邓逸冰; 姬志勤
(西北农林科技大学 植物保护学院，陕西杨凌712100)
Author(s):
GUO Zheng; LI Wan-wan; YANG Han; DENG Yi-bing; JI Zhi-qin
(College of Plant Protection, Northwest A&F University, Yangling 712100，China)
关键词:
月季; 内生放线菌; 抑菌活性
Keywords:
Rosa chinensis Jacq; entophytic actinomycete; antimicrobial activity
分类号:
S476+.1
DOI:
10.3969/j.issn.1000-4440.2015.02.013
文献标志码:
A
摘要:
植物内生菌作为有待开发的微生物资源在农用抗生素的开发方面具有广阔的应用前景。因此，本研究以月季作为植物材料对植物内生菌进行筛选，得到1株具有抗菌活性的植物内生放线菌GZ-57，并通过大孔吸附树脂，硅胶柱层析以及高效液相色谱等技术对该菌株的发酵滤液进行活性化合物的分离得到2种化合物57-1和57-2，通过1H NMR，13C NMR及ESI-MS等波谱分析，结果表明，这2种化合物分别为水杨酰胺和1H-吡咯-2-甲酰胺。对2种化合物进行抑菌试验研究结果表明，抗真菌方面：在50 μg/ml浓度下，化合物57-1对小麦赤霉病菌、烟草赤星病菌和茄子黄萎病菌菌丝生长抑制率分别为42.4%、35.5%和34.4%；化合物57-2对茄子黄萎病菌和烟草赤星病菌菌丝生长抑制率分别为40.0%和34.8%。抗细菌方面：化合物57-1对7种病原细菌均无明显抑制作用；化合物57-2对烟草青枯病菌、猕猴桃溃疡病菌和蜡状芽孢杆菌有较强的抑制作用，其最小抑菌浓度分别为12.50 μg/ml、6.25 μg/ml和12.50 μg/ml。表明菌株GZ-57发酵产物对多种病原真菌和病原细菌具有抑制活性，且效果明显。
Abstract:
Endophytes as microbial resources to be developed has broad application prospects in the development of agricultural antibiotics. In this study, an antimicrobial endophytic actinomycete, named GZ-57, was isolated from the leaves of Rosa chinensis Jacq. Two compounds, 57-1 and 57-2, acquired through the absorption of macroporous resin, silica gel column chromatography and RP-HPLC were identified as salicylamide and 1H-pyrrole-2-carboxamide, respectively. The inhibition rates of compound 57-1 at 50 μg/ml against the mycelia growth of Fusarium graminearum,Alternaria alternate and Verticillium dahlia were 42.4%, 35.5% and 34.4%,respectively, and the inhibition rates of compound 57-2 against V. dahliae and A. alternate were 40.0% and 34.8%. Compound 57-1 showed no antibacterial activity to seven strains, while compound 57-2 exerted strong antibacterial activities against Ralstonia solanacearum, Pseudomonas syringae and Bacillus cereus, with MIC values being 12.50 μg/ml, 6.25 μg/ml and 12.50 μg/ml, respectively. Due to a variety of inhibitory activities to pathogenic fungi and bacteria, GZ-57 can be applied as a biocontrol endophyte.

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备注/Memo

备注/Memo:
收稿日期：2014-10-20 基金项目：国家自然科学基金项目（31371973、31301700）；陕西省自然科学基金项目（2013JQ3003）；中央高校基本科研业务费专项（QN2013009） 作者简介：国政(1990-)，男，山东聊城人，硕士研究生，研究方向为天然产物化学。（Tel）18292490412;（E-mail）guozheng1271@163.com 通讯作者：姬志勤，（Tel）15309203829;（E-mail）：jizhiqin@nwsuaf.edu.cn
更新日期/Last Update: 2015-04-30