[1]张成玲,赵永强,孙厚俊,等.甘薯卷叶病毒复制相关蛋白部分基因克隆及分析[J].江苏农业学报,2015,(02):298-303.[doi:10.3969/j.issn.1000-4440.2015.02.012]
　ZHANG Cheng-ling,ZHAO Yong-qiang,SUN Hou-jun,et al.Cloning and sequence analysis of replication-associated protein partial genes of sweet potato leaf curl virus isolates[J].,2015,(02):298-303.[doi:10.3969/j.issn.1000-4440.2015.02.012]
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甘薯卷叶病毒复制相关蛋白部分基因克隆及分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
298-303
栏目:
植物保护
出版日期:
2015-04-30

文章信息/Info

Title:
Cloning and sequence analysis of replication-associated protein partial genes of sweet potato leaf curl virus isolates
作者:
张成玲; 赵永强; 孙厚俊; 杨冬静; 徐振; 谢逸萍
（江苏徐淮地区徐州农业科学研究所，农业部甘薯生物学与遗传育种重点实验室，江苏徐州221131）
Author(s):
ZHANG Cheng-ling; ZHAO Yong-qiang; SUN Hou-jun; YANG Dong-jing; XU Zhen; XIE Yi-ping
(Xuzhou Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province/Key Laboratory of Biology and Genetic Improvement of Sweet Potato, Ministry of Agriculture, Xuzhou 221131, China)
关键词:
甘薯卷叶病毒; AC1基因; 克隆; 序列分析
Keywords:
sweetpotato leaf curl virus; AC1 gene; cloning; sequence analysis
分类号:
S531
DOI:
10.3969/j.issn.1000-4440.2015.02.012
文献标志码:
A
摘要:
为明确甘薯卷叶病毒基因的分子特性及其变异，从江苏省徐州市表现卷叶、黄化等症状的甘薯上获得5个分离物(SPLCV1-1、SPLCV2-2、SPLCV3-1、SPLCV3-2、SPLCV4-1),利用PCR方法扩增克隆了这5个分离物的AC1部分基因序列，进行分析。结果表明，扩增到的5个分离物基因序列均为452个碱基，核苷酸序列一致率较高，为91.8%~99.8%，氨基酸序列一致率为94.0%~100.0%，且氨基酸序列的变异集中在 143~166位点。在构建的系统进化树上，SPLCV分离物分为2个组，其中组Ⅰ包括日本、韩国、印度、巴西、美国及中国的部分分离物，本研究所得到的4个分离物也位于该组中，其中SPLCV2-2、SPLCV3-1和本研究室2012年得到的江苏省邳州港上分离物SPLCV5-1聚为一簇；组Ⅱ分离物均为中国分离物，且本研究得到的分离物SPLCV3-2也位于该组中。
Abstract:
To characterize the gene and gene variation of sweet potato leaf curl virus (SPLCV) infecting Ipomoea batatas in China, sweet potatoes showing yellow vein and curl leaf symptoms were collected from Xuzhou， Jiangsu province, and AC, gene fragments of five isolates from sweet potato named SPLCV1-1, SPLCV2-2, SPLCV3-1, SPLCV3-2, SPLCV4-1 were amplified by PCR and analyzed. The nucleotide sequences and amino acid sequences showed similarieties of 91.8%-99.8% and 94.0%-100.0%, respectively for the five isolates containing 452 base pairs and 150 amino acids. The variation of amino acid accurred in loci 143-166 of the AC1 gene. Phylogenetic trees constructed with Japanese, Korean, Indian and Chinese isolates revealed that the isolates were divided into two groups. Group Ⅰ included the isolates collected from Japan, Korea, India, Brazil，America and China, and four isolates obtained in this study, SPLCV1-1, SPLCV2-2, SPLCV3-1, and SPLCV4-1). SPLCV2-2, SPLCV3-1, and SPLCV5-1 collected from Pizhou, Jiangsu province, were grouped into a cluster. Isolates in group came from China, in cluding SPLCV3-2.

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备注/Memo

备注/Memo:
收稿日期：2014-10-30 基金项目：江苏省农业科技自主创新探索性项目[CX(13)5080]；江苏省自然科学基金项目（BK20140230）；国家甘薯产业技术体系项目（CARS-11-B-09） 作者简介：张成玲（1983-），女，山东沂源人，博士，副研究员，主要从事甘薯病虫害研究。（E-mail）zhchling5291@163.com 通讯作者：谢逸萍,(E-mail)xieyiping6216@163.com
更新日期/Last Update: 2015-04-30