[1]杨培新,郑锐东,罗集丰,等.1 株产纤维素酶真菌的筛选鉴定及其产酶条件优化[J].江苏农业学报,2015,(01):186-191.[doi:10.3969/j.issn.1000-4440.2015.01.029]
　YANG Pei-xin,ZHENG Rui-dong,LUO Ji-feng,et al.Identification of a cellulase-decomposing fungus and its enzyme-producing conditions[J].,2015,(01):186-191.[doi:10.3969/j.issn.1000-4440.2015.01.029]
点击复制

1 株产纤维素酶真菌的筛选鉴定及其产酶条件优化()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
186-191
栏目:
加工贮藏·质量安全
出版日期:
2015-02-28

文章信息/Info

Title:
Identification of a cellulase-decomposing fungus and its enzyme-producing conditions
作者:
杨培新1; 郑锐东2; 罗集丰1; 谢桂勉1; 郑钢勇2; 方培炜3; 蔡佳维3
（1.揭阳职业技术学院生物工程系，广东揭阳522000；2.揭阳职业技术学院实训中心，广东揭阳522000；3.揭阳职业技术学院师范教育系，广东揭阳522000）
Author(s):
YANG Pei-xin1; ZHENG Rui-dong2; LUO Ji-feng1; XIE Gui-mian1; ZHENG Gang-yong2; FANG Pei-wei3; CAI Jia-wei3
（1.Bioengineering Department, Jieyang Vocational and Technical College, Jieyang 522000, China；2.Training Center, Jieyang Vocational and Technical College, Jieyang 522000, China;3.Normal Education Department, Jieyang Vocational and Technical College, Jieyang 522000, China）
关键词:
纤维素降解菌; 筛选; 鉴定; ITS; 产酶条件; 响应面法
Keywords:
cellulose-decomposing fungus; screening; identification; ITS; enzyme-producing condition; response surface method
分类号:
Q936
DOI:
10.3969/j.issn.1000-4440.2015.01.029
文献标志码:
A
摘要:
为筛选新的高产纤维素酶真菌，从长期堆放生物质废弃物土壤中分离到1株产纤维素酶真菌。经形态特征观察、ITS分析，初步鉴定为曲霉属，暂定名为Aspergillus cel403。通过单因素试验研究了不同碳源、氮源浓度和培养基初始pH值对该菌在液体发酵中产纤维素酶的影响，在此基础上运用响应面法分析其最佳发酵条件。结果表明，Aspergillus cel403 产纤维素酶最佳条件：培养基组分为CMC-Na 15.60 g、KH2PO4 1.00 g、MgSO4·7H2O 0.50 g、NaCl 0.10 g、NaNO3 2.50 g、FeCl3 1 mg、CaCl2 0.10 g、酵母膏1.09 g，H2O 1 000 ml，pH7.1；30 ℃、140 r/min摇床培养6 d。该条件下发酵产纤维素酶活性为89.66 U/ml，比未经优化发酵条件对照提高了15.02%。可见，Aspergillus cel403 在纤维素降解利用方面具备进一步开发潜力。
Abstract:
To screen and characterize novel fungi with powerful cellulose degradation capability, a cellulose-decomposing fungus was isolated from soil rich in biomass wastes. Based on morphology and ITS sequence analysis, the fungus was initially classified into the genus of Aspergillus and temporarily named as strain cel403. The influence of carbon and nitrogen sources concentrations, and initial pH were studied through single factor experiment on the cellulase production. The response surface method was used to analyse its optimum fermentation condition for cel403, which was shown as follows: the medium was comprised of CMC-Na 15.60 g, KH2PO4 1.00 g, MgSO4·7H2O 0.50 g, NaCl 0.10 g, NaNO3 2.50 g, FeCl3 1 mg, CaCl2 0.10 g, yeast extract 1.09 g, H2O 1 000 ml, with pH value at 7.1, and cel403 was cultured at 30 ℃ for 6 d.The cellulose production under above conditions was 89.66 IU/ml, increased by 15.02% compared to control. Aspergillus cel403 is a candidate strain for the degradation of cellulose.

参考文献/References:

[1]孟建宇,冯福应,刘向红,等. 1株纤维素降解菌的筛选及产酶条件研究[J].微生物学杂志,2011,31(4):47-49.
[2]范晓娟，朱红梅，韩士群，等．白腐菌对水葫芦木质纤维素的降解及对厌氧发酵的影响[J]．江苏农业学报，2013，29(5)：1043-1050．
[3]王明月，毕荣璐，王金华，等．板栗苞壳纤维素降解真菌的筛选及降解效果[J]．江苏农业科学，2014，42(5)：290-293.
[4]陈华，廖崇静，冯珊，等．酸碱处理提取水稻秸秆纤维素的研究[J]．江苏农业科学，2014，42(6)：252-254.
[5]王璐，张海红，杨柳，等．3株纤维素分解真菌的分子鉴定[J]．江苏农业科学，2013，41(8)：51-53.
[6]林海龙,武国庆,罗虎,等.我国纤维素燃料乙醇产业发展现状[J].粮食与饲料工业,2011(1):31.
[7]REGINA V, ALGIMANTAS P, VITA R. Cellulose degradation in rye straw by micromycetes and their complexes[J]. Ekologija, 2008, 54(1):29.
[8]杨晓宸,卢雪梅,黄峰. 木质纤维素微生物转化机理研究进展[J].纤维素科学与技术,2007,15(1):52-57.
[9]费岚,邵飞,贾睿,等.浒苔纤维素分解菌株的筛选、鉴定与纤维素酶活特性研究[J].上海海洋大学学报,2014,3(23):412.
[10]方诩,秦玉琪,李雪芝,等.纤维素酶与木质纤维素生物降解转化的研究进展[J].生物工程学报,2010,26(7):865.
[11]PHITSUWAN P, LAOHAKUNJIT N, KERDCHOECHUEN O, et al. Present and potential applications of cellulases in agriculture, biotechnology and bioenergy[J].Folia Microbiological,2013,58(2):163-176.
[12]石文卿,陶能国,刘跃进,等. 一株高产纤维素酶真菌的分离及产酶特性研究[J].环境工程学报,2011,6(5):1435-1440.
[13]张粲,武改红,赫荣琳,等.纤维素酶高产菌株桧状青霉9-3的诱变选育及产酶条件研究[J].基因组学与应用生物学,2014,33(3):568-569.
[14]朱晓媛,胡铁,张蕾,等. Penicillium sp.1407产纤维素酶的发酵条件优化[J].中国粮油学报,2014,29(5):115-117.
[15]黄达明,张雪,管国强,等.响应面法优化混菌发酵豆粕的条件研究[J].食品工业科技, 2014(13):224-228.
[16]张纪忠.真菌鉴定手册[M].上海:复旦大学出版社,1990.
[17]AKHIR S M, ABD-AZIZI S, SALLEH M M, et al. Bacillus licheniformis TH-1 by response surface methods[J]. Biotechnology, 2009,8(1):120-125.
[18]SHI L E, YING G Q, ZHANG X Y, et al. Medium optimization for 5′-phosphodiesterase production from Penicillium citrinum using response surface methodology[J]. Food Technol Biotechnol, 2007, 45(2):126-133.

相似文献/References:

[1]王恒波,余泽怀,肖乃衍,等.转基因番木瓜检测方法的建立[J].江苏农业学报,2018,(05):1198.[doi:doi:10.3969/j.issn.1000-4440.2018.05.032]
　WANG Heng-bo,YU Ze-huai,XIAO Nai-yan,et al.Establishment of detection method for transgenic papaya[J].,2018,(01):1198.[doi:doi:10.3969/j.issn.1000-4440.2018.05.032]

备注/Memo

备注/Memo:
收稿日期：2014-08-29 基金项目：广东省2012科技计划项目（2012B020420003）；揭阳职业技术学院2013科研重点课题（JYCKZ1306）；揭阳职业技术学院2014~2016年“创新强校”自主能力提升项目 作者简介：杨培新（1971-），男，广东揭阳人，硕士，教授，主要从事农业生物技术相关教学与研究工作。 通讯作者：郑锐东，（E-mail）superzrd@163.com
更新日期/Last Update: 2015-02-28