[1]徐引弟,蔡旭旺,徐晓娟,等.副猪嗜血杆菌血清7型crp基因缺失株的部分生物学特性[J].江苏农业学报,2023,(06):1372-1379.[doi:doi:10.3969/j.issn.1000-4440.2023.06.013]
　XU Yin-di,CAI Xu-wang,XU Xiao-juan,et al.Partial biological characteristics of Haemophilus parasuis serotype 7 crp gene deletion strain[J].,2023,(06):1372-1379.[doi:doi:10.3969/j.issn.1000-4440.2023.06.013]
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副猪嗜血杆菌血清7型crp基因缺失株的部分生物学特性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年06期
页码:
1372-1379
栏目:
畜牧兽医·水产养殖
出版日期:
2023-09-30

文章信息/Info

Title:
Partial biological characteristics of Haemophilus parasuis serotype 7 crp gene deletion strain
作者:
徐引弟1; 蔡旭旺2; 徐晓娟2; 王治方1; 张家庆1; 朱文豪1; 雷亚楠1; 张立宪1; 李海利1; 焦文强1; 王克领1
(1.河南省农业科学院畜牧兽医研究所，河南郑州450002;2.华中农业大学动物医学院农业微生物国家重点实验室，湖北武汉430070)
Author(s):
XU Yin-di1; CAI Xu-wang2; XU Xiao-juan2; WANG Zhi-fang1; ZHANG Jia-qing1; ZHU Wen-hao1; LEI Ya-nan1; ZHANG Li-xian1; LI Hai-li1; JIAO Wen-qiang1; WANG Ke-l
(1.Institute of Animal Husbandry and Veterinary Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;2.State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China)
关键词:
副猪嗜血杆菌血清7型; crp基因缺失株; 生长特性; 抗药性; 毒力
Keywords:
Haemophilus parasuis serotype 7; crp gene deletion strain; growth characteristics; drug resistance; virulence
分类号:
S858.31
DOI:
doi:10.3969/j.issn.1000-4440.2023.06.013
文献标志码:
A
摘要:
副猪嗜血杆菌(HPS)是引起猪格拉瑟病(Glasser’s disease)的病原菌，给全球和中国规模化养猪业造成了重大经济损失。副猪嗜血杆菌血清型众多，其中血清7型是近年来分离比例越来越高的血清型，也是危害越来越严重的血清型。crp是最重要的系统调控因子之一，在细菌感染过程中对细菌适应环境变化起着至关重要的作用。为了筛选副猪嗜血杆菌血清7型crp基因缺失弱毒株，采用自然转化法构建了副猪嗜血杆菌血清7型临床分离株HPS7的crp基因缺失株，对HPS7及其crp基因缺失株的生长特性、生物膜形成、抗药性、毒力等进行了研究。结果表明，HPS7的crp基因缺失后，生长显著减慢，自凝速度减慢，生物膜形成能力明显减弱，对大部分抗生素的抗性降低，对豚鼠的毒力降低。以上结果说明crp基因对HPS7的生长、抗药性、毒力均有明显影响。本研究结果为筛选副猪嗜血杆菌血清7型弱毒株提供了参考。
Abstract:
Haemophilus parasuis (HPS) is the pathogen that causing porcine Glasser’s disease， causing great economic losses to the large scale pig industry in the world and China. There are many serotypes of HPS, serotype 7 is a serotype with increasing proportion of isolation in recent years, and also a serotype with more and more serious harm. The crp is one of the most important systemic regulatory factors and plays a crucial role in adapting to environmental changes during bacterial infection. In order to screen HPS serotype 7 crp gene deletion attenvated strain, the crp gene deletion strain of HPS serotype 7 clinical isolate HPS7 was constructed by natural transformation method. Growth characteristics, biofilm formation, drug resistance and virulence of HPS7 and crp deletion strain were studied. The results showed that the growth speed was significantly reduced, autoagglutination speed was showed down, the ability of biofilm formation was obviously weakened, resistance to most antibiotics was reduced, and the virulence to guinea pig was decreased after crp was deleted in HPS7. The results showed that crp gene had significant influence on growth, drug resistance and virulence of HPS7. The results of this study provide a reference for the screening of HPS serotype 7 attenuated strains.

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备注/Memo

备注/Memo:
收稿日期:2023-03-11基金项目:国家重点研发计划项目(2021YFD1301205)；河南省科技攻关项目(212102110378)；河南省农业科学院自主创新项目(2022ZC43)作者简介:徐引弟(1974-)，女，湖北浠水人，博士，副研究员，主要从事动物病原微生物学研究。(E-mail)445177674@qq.com
更新日期/Last Update: 2023-11-17