[1]陈瑶瑶,严良文,刘建汀,等.黄秋葵果实发育过程中细胞壁组成成分、糖代谢及相关酶活性的变化[J].江苏农业学报,2023,(05):1217-1224.[doi:doi:10.3969/j.issn.1000-4440.2023.05.014]
　CHEN Yao-yao,YAN Liang-wen,LIU Jian-ting,et al.Developmental changes of okra fruits in cell wall composition, saccharometabolism and related enzymatic activities[J].,2023,(05):1217-1224.[doi:doi:10.3969/j.issn.1000-4440.2023.05.014]
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黄秋葵果实发育过程中细胞壁组成成分、糖代谢及相关酶活性的变化()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年05期
页码:
1217-1224
栏目:
园艺
出版日期:
2023-08-31

文章信息/Info

Title:
Developmental changes of okra fruits in cell wall composition, saccharometabolism and related enzymatic activities
作者:
陈瑶瑶1; 严良文1; 刘建汀2; 刘智成1; 余洁3; 郑作芸1; 朱海生2
(1.龙岩市农业科学研究所,福建龙岩364000；2.福建省蔬菜遗传育种重点实验室,福建福州350013；3.龙岩市新罗区农业局,福建龙岩364000）
Author(s):
CHEN Yao-yao1; YAN Liang-wen1; LIU Jian-ting2; LIU Zhi-cheng1; YU Jie3; ZHENG Zuo-yun1; ZHU Hai-sheng2
（1.Longyan Institute of Agricultural Sciences, Longyan 364000, China;2.Fujian Key Laboratory of Vegetable Genetics and Breeding, Fuzhou 350013, China;3.Agricultural Bureau of Xinluo District, Longyan 364000, China）
关键词:
黄秋葵; 果实发育; 细胞壁组成成分; 糖代谢; 糖代谢酶活性
Keywords:
okra; fruit development; cell wall composition; saccharometabolism; activity of enzymes related to saccharometabolism
分类号:
S649
DOI:
doi:10.3969/j.issn.1000-4440.2023.05.014
文献标志码:
A
摘要:
以黄秋葵(Abelmoschus esculentus L.)卡里巴和油绿一号果实为试验材料,测定果实发育过程中细胞壁组成成分、糖分积累及相关酶活性的变化,并对纤维素含量与纤维素代谢酶活性进行了相关性分析。结果表明：两个品种黄秋葵果实的适宜采收期为花后6～7 d,在果实发育过程中木质素和半纤维素含量均先上升后下降,纤维素含量不断上升,纤维素合酶（CesA）和纤维素酶(Kor)活性不断上升,蔗糖合酶（SS-I)、内切葡聚糖酶(Cx)和外切葡聚糖酶(C1)活性先升高后降低,β-葡萄糖苷酶（β-GC）活性不断降低。卡里巴果实中木质素、半纤维素、纤维素含量和纤维素代谢相关酶活性均高于油绿一号。相关性分析结果表明,CesA和Kor酶活性与纤维素含量呈显著正相关,β-GC酶活性与纤维素含量呈极显著负相关。糖分含量测定结果显示,两个品种果实中的蔗糖含量均呈上升趋势,果糖与葡萄糖的含量均在花后6 d达到峰值,果实中蔗糖磷酸合成酶（SPS）和蔗糖转化酶（SAI、BAI、NI）活性的峰值期均出现在果实老化前。综上所述,纤维素含量不断增加是影响黄秋葵果实老化的主要因素,CesA、Kor和β-GC为参与纤维素代谢的关键酶类,蔗糖代谢酶参与了黄秋葵果实的成熟老化过程。
Abstract:
The fruits of two okra (Abelmoschus esculentus L.) varieties, Kariba and Youlü 1, were used as experimental materials to determine the changes of cell wall composition, sugar accumulation and related enzyme activities during fruit development. The correlation between cellulose content and the activities of enzymes related to cellulose metabolism was analyzed. The results showed that the suitable harvest time of the two varieties of okra was 6-7 days after anthesis. During the fruit development, the contents of lignin and hemicellulose increased first and then decreased, the content of cellulose increased continuously, the activities of cellulase synthase （CesA） and korrigan (Kor) increased, the activities of sucrose synthase (SS-I), endo-β-1,4-glucanase (Cx) and exo-β-1,4-glucanase (C1) increased first and then decreased, and the activity of β-glucosidase (β-GC) decreased continuously. The contents of lignin, hemicellulose, cellulose and the activities of enzymes related to cellulose metabolism in Kariba fruit were higher than those in Youlü 1. The results of correlation analysis showed that the activities of CesA and Kor were positively correlated with the content of cellulose, and the activity of β-GC was negatively correlated with the content of cellulose. The results of sugar content determination indicated that the content of sucrose in the fruits of the two varieties showed an upward trend, the contents of fructose and glucose reached the peak values at six days after anthesis, and the activities of sucrose phosphate synthase(SPS) and invertase (SAI, BAI, NI) reached the peak values before fruit aging. In conclusion, the increasing cellulose content was the main factor affecting the aging of okra fruit. CesA, Kor and β-GC were the key enzymes involved in the metabolism of cellulose, and sucrose metabolism was involved in the aging of okra fruit.

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备注/Memo

备注/Memo:
收稿日期：2023-01-10 基金项目：福建省蔬菜遗传育种重点实验室开放基金项目（FJVRC-2022-01、FJVRC-2023-03）；福建省科技项目（2018N0060）；福建省个人科技特派员项目（202335080191）作者简介：陈瑶瑶（1983-）,女,福建龙岩人,硕士,助理研究员,从事蔬菜生理与栽培研究。（E-mail）huanan_1983@163.com 通讯作者：朱海生,（E-mail）zhs@163.com
更新日期/Last Update: 2023-09-13