[1]张铭芳,李卉,韩东洋,等.不同百合种质资源对灰霉病的抗性[J].江苏农业学报,2022,38(04):1078-1084.[doi:doi:10.3969/j.issn.1000-4440.2022.04.026]
　ZHANG Ming-fang,LI Hui,HAN Dong-yang,et al.Resistance of different lily germplasm resources to Botrytis cinerea[J].,2022,38(04):1078-1084.[doi:doi:10.3969/j.issn.1000-4440.2022.04.026]
点击复制

不同百合种质资源对灰霉病的抗性()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年04期
页码:
1078-1084
栏目:
园艺
出版日期:
2022-08-31

文章信息/Info

Title:
Resistance of different lily germplasm resources to Botrytis cinerea
作者:
张铭芳1; 李卉2; 韩东洋1; 杜运鹏1; 薛静1; 高俊莲1; 张秀海1
(1.北京市农林科学院草业花卉与景观生态研究所，农业农村部华北都市农业重点实验室，北京100097；2.北京林业大学园林学院，北京100083）
Author(s):
ZHANG Ming-fang1; LI Hui2; HAN Dong-yang1; DU Yun-peng1; XUE Jing1; GAO Jun-lian1; ZHANG Xiu-hai1
（1.Institute of Grassland, Flowers and Ecology， Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs, Beijing 100097, China;2.School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China）
关键词:
百合; 灰霉菌; 抗病性; 活性氧; 防御酶
Keywords:
Lilium spp.; Botrytis cinerea; disease resistance; reactive oxygen species (ROS); defense enzyme
分类号:
S682.2
DOI:
doi:10.3969/j.issn.1000-4440.2022.04.026
文献标志码:
A
摘要:
灰霉病是百合的主要病害之一，严重影响百合生产，筛选高抗种质资源是百合抗病育种的重要途径之一。本研究对不同百合种质资源叶片进行离体接种，结合扫描电镜观察，并进行活性氧、相关酶活性的测定。结果表明，卷丹百合属于高感（HS）种质，岷江百合-1、岷江百合-2、杂交种2065-1属于中抗（R）种质，宜昌百合、湖北百合、Champion Diamonds、雷林石、Craming Diamonds、红唇、杂交种2065-22属于高抗（HR）种质。接种灰霉菌后，高抗种质湖北百合叶片中过氧化氢含量变化更为剧烈，同时超氧阴离子含量会出现2次峰值，而高感种质卷丹百合则只有1次峰值。接种灰霉菌后，百合叶片中超氧化物歧化酶（SOD）活性与百合抗病性具有相关性。
Abstract:
Grey mold is one of the main diseases of lily, which seriously affects lily production. Thus, screening of high resistant lily germplasm is an important aspect of lily disease-resistant breeding. In this study, the leaves of different lily germplasm resources were inoculated in vitro, leaf structure and conidia growth condition were observed through scanning electron microscopy, and reactive oxygen species (ROS) and the related enzyme activities were determined. The results indicated that Lilium lancifolium belonged to the high-sensitivity (HS) germplasm. Lilium regale-1, Lilium regale-2 and the hybrid 2065-1 belonged to the middle resistance (R) germplasms. Lilium leucanthum, Lilium henryi, Champion Diamonds, Lellin Stone, Craming Diamonds, Red Lips, hybrid 2065-22 belonged to high resistance (HR) germplasms. After inoculation with Botrytis cinerea, the content of H2O2 in leaves of high resistant germplasm Lilium henryi changed dramatically, and the content of superoxide anion showed two peaks, while only one peak was found in Lilium lancifolium. After inoculation with Botrytis cinerea, the activity of superoxide dismutase (SOD) in lily leaves was correlated with the disease resistance.

参考文献/References:

[1]蒋瑶，陈文波，蒋炷宇. 亚洲百合不定芽的诱导及再生植株的建立[J]. 江苏农业科学，2017,45 (12):35-38.
[2]刘高峰，巨秀婷，唐楠，等. 不同培养基质和肥力水平对兰州百合小鳞茎生长的影响[J]. 江苏农业学报，2021, 37（3）：718-723.
[3]徐琼，徐秉良，王芳. 观赏百合叶枯病症状类型与病原菌鉴定[J]. 植物保护，2006，32(5):61-64.
[4]朱丽梅，罗凤霞，李明凤，等. 底特律等8个百合品种对百合灰霉病的抗病性鉴定[J]. 江苏农业科学，2012,40(5):81-83.
[5]朱丽梅，胡凤荣，罗凤霞. 不同百合品种对百合灰霉病的抗病性鉴定[J]. 植物保护，2010,36(3):148-151.
[6]WILLIAMSON B, TUDZYNSKI B, TUDZYNSK I P, et al. Botrytis cinerea: the cause of grey mold disease[J]．Molecular Plant Pathology，2007,8(5):561-580.
[7]COLLADO I G, GALN R H, PATRON R D, et al. Metabolites from a shake culture of Botrytis cinerea[J]. Phytochemistry，1995,38（3）：647-650.
[8]MARUMO S, KATAYAMA M, KOMORI E, et al. Microbial production of abscisic acid by Botrytis cinerea[J]. Agricultural and Biological Chemistry, 1982,46(7):1967-1968.
[9]CUTLER H G, JACYNO J M, HARWOOD J S, et al. Botcinolide: a biologically active natural product from Botrytis cinerea[J]. Bioscience, Biotechnology, and Biochemistry，1993,57(11):1980-1982.
[10]MOYAHEDI S, HEALE J B. The roles of aspartic proteinase and endo-pectin lyase enzymes in the primary stages of infection and pathogenesis of various host tissues by different isolates of Botrytis cinerea Pers ex. Pers. [J]. Physiological and Molecular Plant Pathology，1990,36(4):303-324.
[11]ZHU L, HU F, LUO F. Identification of disease resistance of Lilium species to lily gray mould[J]. Plant Protection，2011, 36（3）:148-151.
[12]JIA M, DI W, LIU Y, et al. ROS-induced oxidative stress in nobile-type dendrobium protocorm-like bodies (plbs) during verification[J]. Cryo-Letters，2016,37(4):253-263.
[13]YIN H, CHEN Q, YI M. Effects of short-term heat stress on oxidative damage and responses of antioxidant system in Lilium longiflorum [J]. Plant Growth Regulation，2008,54:45-54.
[14]LI H S. Principles and techniques of plant physiological biochemical experiment[M]. Beijing：Higher Education Press，2000: 120-122.
[15]CAO J， JIANG W， ZHAO Y. Experiment guidance of postharvest physiology and biochemistry of fruits and vegetables[M].Beijing: Chinese Light Industry Press，2007: 118-120.
[16]康立功，齐凤坤，许向阳，等. 番茄叶片蜡质和角质层与芝麻斑病菌侵染的关系[J].中国蔬菜，2010(18):47-50.
[17]KEPPLER D，BAKER C J. O2·- -initiated lipid peroxidation in a bacteria-induced hypersensitive reaction in tobacco cell suspensions[J]. Phytopathology，1989,79（5）:555-562.
[18]陶宗娅，田小凤，向述莲，等．赤霉菌粗毒素对小麦幼苗活性氧代谢的影响[J]. 西南农业大学学报（自然科学版），2005,27(6)：851-856.
[19]赵嫚,陈仕勇,李亚萍,等. 外源GABA对盐胁迫下金花菜种子萌发及幼苗抗氧化能力的影响[J].江苏农业学报,2021,37(2):310-316.
[20]里程辉,于辉,刘志,等.淹水胁迫下不同中间砧对苹果岳冠叶片和根系抗氧化酶和非酶类抗氧化物活性的影响[J].江苏农业科学,2021,49(3):121-125.
[21]高芬，褚建梅，李静虹，等．植物病原真菌致病机理研究进展[J]. 江苏农业学报，2014,30(5):1174-1179.
[22]葛秀春，宋凤鸣，郑重．稻瘟菌侵染后水稻幼苗活性氧的产生与抗病性的关系[J]. 植物生理学报，2000, 26(3):227-231.
[23]刘丽婷．葡萄品种资源叶片白腐病和灰霉病的抗病鉴定及评价[D].沈阳：沈阳农业大学，2018.

相似文献/References:

[1]曹兴,郭尚敬,高祥斌,等.百合转录辅激活因子LlMBF1c基因的克隆与表达分析[J].江苏农业学报,2018,(05):1120.[doi:doi:10.3969/j.issn.1000-4440.2018.05.022]
　CAO Xing,GUO Shang-jing,GAO Xiang-bin,et al.Cloning and expression analysis of LlMBF1c in lily[J].,2018,(04):1120.[doi:doi:10.3969/j.issn.1000-4440.2018.05.022]

备注/Memo

备注/Memo:
收稿日期：2021-12-07基金项目：北京市农林科学院科技创新能力建设专项（KJCX20200103）；国家重点研发计划项目（2020YFD1000400）；北京市农林科学院青年基金项目（QNJJ201910）作者简介：张铭芳（1987-），女，湖南溆浦人，博士，助理研究员，主要从事百合分子育种研究。（E-mail）qioki1987@163.com通讯作者：张秀海，（E-mail）zhangxiuhai@baafs.net.cn
更新日期/Last Update: 2022-09-06