[1]倪萌,王娟,王爽,等.克服萝卜自交不亲和性的化学试剂筛选[J].江苏农业学报,2022,38(04):1042-1048.[doi:doi:10.3969/j.issn.1000-4440.2022.04.022]
　NI Meng,WANG Juan,WANG Shuang,et al.Screening of chemical reagents for overcoming the self-incompatibility of radish[J].,2022,38(04):1042-1048.[doi:doi:10.3969/j.issn.1000-4440.2022.04.022]
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克服萝卜自交不亲和性的化学试剂筛选()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年04期
页码:
1042-1048
栏目:
园艺
出版日期:
2022-08-31

文章信息/Info

Title:
Screening of chemical reagents for overcoming the self-incompatibility of radish
作者:
倪萌1; 王娟1; 王爽1; 梅燚2; 柳李旺1; 徐良1; 王燕1
（1.南京农业大学作物遗传与种质创新国家重点实验室/农业农村部华东地区园艺作物生物学与种质创新重点实验室/园艺学院，江苏南京210095；2.江苏沿海地区农业科学研究所，江苏盐城224002）
Author(s):
NI Meng1; WANG Juan1; WANG Shuang1; MEI Yi2; LIU Li-wang1; XU Liang1; WANG Yan1
（1.State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China)，Ministry of Agriculture and Rural Affairs/College of Horticulture， Nanjing Agricultural University， Nanjing 210095，China；2.Institute of Agricultural Sciences in the Coastal District of Jiangsu Province， Yancheng 224002， China）
关键词:
萝卜; 自交不亲和性; 化学试剂; 花粉萌发; 活性氧
Keywords:
radish; self-incompatibility; chemical reagent; pollen germination; reactive oxygen species
分类号:
S631.1
DOI:
doi:10.3969/j.issn.1000-4440.2022.04.022
文献标志码:
A
摘要:
为探讨克服萝卜自交不亲和性的有效化学方法，本研究选用不同浓度的槲皮素（Quercetin，QUE）、油菜素内酯（Bassinolide，BR）、褪黑素（Melatonin，MT）和激动素（Kinetin，KT）4种化学试剂对3个萝卜自交不亲和系（NAU-YXYB、NAU-LV791、NAU-WXQ）进行花期柱头喷施处理。通过亲和指数、花粉体外萌发及柱头活性氧（Reactive oxygen species，ROS）强度测定，筛选克服萝卜自交不亲和性的有效方法。结果表明，4种试剂处理均能在一定程度上克服萝卜的自交不亲和性，其中1.5 mmol/L QUE效果最佳，其次是100 μmol/L MT和100 mg/L KT。本研究结果为建立高效的萝卜自交不亲和系亲本繁殖技术体系提供参考。
Abstract:
In order to explore the effective chemical methods for overcoming the self-incompatibility of radish, four chemical reagents including quercetin（QUE）， brassinolide（BR）， melatonin（MT）and kinetin（KT）with different concentrations were used to spray the stigma of three self-incompatibility radish lines at flowering stage, such as NAU-YXYB， NAU-LV791 and NAU-WXQ. The effective methods to overcome self-incompatibility of radish were screened by affinity index， pollen germination in vitro and intensity of stigma reactive oxygen species (ROS). The results showed that the four reagents could overcome the self-incompatibility of radish to some extent, and 1.5 mmol/L QUE had the best effect, followed by 100 mol/L MT and 100 mg/L KT. These results can provide a reference for the establishment of efficient technology system for parental reproduction of radish self-incompatibility.

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备注/Memo

备注/Memo:
收稿日期：2021-12-30基金项目：中央高校基本业务费项目（KYZZ2022004）；江苏省农业科技自主创新基金项目[CX（19）3045]；江苏高校优势学科建设工程项目（PAPD）作者简介：倪萌（1997-），女，山东枣庄人， 硕士研究生，主要从事蔬菜分子遗传育种工作。通讯作者：王燕，（E-mail）wangyanhs@njau.edu.cn
更新日期/Last Update: 2022-09-06