[1]孔祥震,郭海娟,马放,等.生物絮凝剂改性菌丝球负载好氧反硝化菌T13强化脱氮[J].江苏农业学报,2022,38(02):377-386.[doi:doi:10.3969/j.issn.1000-4440.2022.02.011]
　KONG Xiang-zhen,GUO Hai-juan,MA Fang,et al.Enhanced nitrogen removal by bioflocculant combined mycelial pellets loaded with aerobic denitrificans T13[J].,2022,38(02):377-386.[doi:doi:10.3969/j.issn.1000-4440.2022.02.011]
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生物絮凝剂改性菌丝球负载好氧反硝化菌T13强化脱氮()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年02期
页码:
377-386
栏目:
耕作栽培·资源环境
出版日期:
2022-04-30

文章信息/Info

Title:
Enhanced nitrogen removal by bioflocculant combined mycelial pellets loaded with aerobic denitrificans T13
作者:
孔祥震1; 郭海娟2; 马放1; 耿明月1; 肖霄1
（1.哈尔滨工业大学城市水资源与水环境国家重点实验室，黑龙江哈尔滨150090；2.辽宁大学环境学院，辽宁沈阳110000）
Author(s):
KONG Xiang-zhen1; GUO Hai-juan2; MA Fang1; GENG Ming-yue1; XIAO Xiao1
（1.State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150090, China；2.School of Environment, Liaoning University, Shenyang 110000, China）
关键词:
改性菌丝球; 强化脱氮; 好氧反硝化菌; 生物载体
Keywords:
modified mycelial pellets; enhanced nitrogen removal; aerobic denitrifying bacterium; biomass carrier
分类号:
X52
DOI:
doi:10.3969/j.issn.1000-4440.2022.02.011
文献标志码:
A
摘要:
由黑曲霉Y3(Aspergillus niger Y3)形成的菌丝球经过生物絮凝剂改性强化后作为生物载体固定好氧反硝化菌T13 (Pseudomonas sp. T13)，对其固定前后对全氮的去除效能、菌群的活性进行研究并分析固定化对于生物强化脱氮效果的影响。结果表明，菌丝球负载T13后去除全氮效能得到了明显的提高。当改性菌丝球（湿质量）与T13菌液（3.5×108CFU/ml）的质量体积比 为1∶15时，去除全氮的效能最好。改性菌丝球负载T13后的表观图像和电镜扫描图像(SEM)显示，T13细菌附着在改性菌丝球的表面，且改性菌丝球载体的结构可以连续培养45 d后仍保持紧实结构。傅里叶红外光谱(FTIR)和三维荧光光谱(EEM)结果显示，胞外聚合物(EPS)在改性菌丝球固定T13的过程中起到了关键作用。菌丝球的菌丝缠绕形成的结构提供了强有力的机械结构，且具有较大的比表面积和良好的传质性能，改性菌丝球可以作为良好的生物载体用于生物强化。
Abstract:
After modifying and strengthening of mycelial pellets formed of Aspergillus niger Y3 by bioflocculant, the mycelial pellets were used as bio-carriers to immobilize aerobic denitrifying bacterium T13 (Pseudomonas sp. T13). Removing capabilities of total nitrogen (TN) and microflora activities of mycelial pellets before and after immobilizing of Pseudomonas sp. T13 were studied, and the influence of immobilization on the denitrification effect strengthened by biological factors was analyzed. The results showed that, the removal efficiency of mycelial pellets in removing TN was effectively improved after immobilizing of Pseudomonas sp. T13. The mycelial pellets showed the best effect in TN removing when the mass-volume ratio of modified mycelial pellets (wet weight) to T13 bacterial suspension (3.5×108 CFU/ml) was 1∶15. Photograph and scanning electron microscope (SEM) images of modified mycelial pellets after immobilizing of Pseudomonas sp. T13 revealed that, the T13 bacteria attached on the surface of mycelial pellets, and the structure of modified mycelial pellets maintained firm and intact after continuous cultivation for 45 days. Results of Fourier transform infrared spectroscopy (FTIR) and three-dimensional excitation-emission matrix (EEM) suggested that, extracellular polymer substances (EPS) played an important role in the process of immobilization of T13 by modified mycelial pellets. The structures formed by entangling structure of mycelial pellets provided strong mechanic structure, and had large specific surface area and fine mass transfer performance. Modified mycelial pellets can be used as good bio-carrier for biomass immobilization.

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备注/Memo

备注/Memo:
收稿日期：2021-08-13基金项目：国家自然科学基金项目（52070054）作者简介：孔祥震（1989-），男，安徽宿州人，博士研究生，研究方向为环境微生物、生物强化技术。（E-mail）kxz_19890510@163.com通讯作者：马放，（E-mail）mafang@hit.edu.cn
更新日期/Last Update: 2022-05-07