[1]吴娇,张世青,张凤龙,等.两种无刺蜂蜂胶乙酸乙酯提取物中总酚含量及其抗氧化性比较[J].江苏农业学报,2020,(04):1036-1040.[doi:doi:10.3969/j.issn.1000-4440.2020.04.032]
　WU Jiao,ZHANG Shi-qing,ZHANG Feng-long,et al.Comparison on total phenols content and antioxidant activity of ethyl acetate extracts from two kinds of stingless bee geopropolis[J].,2020,(04):1036-1040.[doi:doi:10.3969/j.issn.1000-4440.2020.04.032]
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两种无刺蜂蜂胶乙酸乙酯提取物中总酚含量及其抗氧化性比较()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年04期
页码:
1036-1040
栏目:
加工贮藏·质量安全
出版日期:
2020-08-31

文章信息/Info

Title:
Comparison on total phenols content and antioxidant activity of ethyl acetate extracts from two kinds of stingless bee geopropolis
作者:
吴娇1; 2; 张世青3; 张凤龙4; 高景林1; 赵冬香1; 赵珊1; 韩文素1; 钟义海1; 王释婕1
(1.中国热带农业科学院环境与植物保护研究所，海南海口571101；2.海南大学热带作物学院，海南海口570000；3.海南大学植物保护学院，海南海口570000；4.云南蜂弘生物科技有限公司，云南昆明650000）
Author(s):
WU Jiao1; 2; ZHANG Shi-qing3; ZHANG Feng-long4; GAO Jing-lin1; ZHAO Dong-xiang1; ZHAO Shan1; HAN Wen-su1; ZHONG Yi-hai1; WANG Shi-jie1
(1.Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;2.College of Tropical Crops, Hainan University, Haikou 570000, China;3.College of Plant Protection, Hainan University, Haikou 570000, China;4.Yunnan Fenghong Biotechnology Co., Ltd., Kunming 650000, China)
关键词:
戈氏无刺蜂; 黄纹无刺蜂; 蜂胶; 乙酸乙酯; 总酚; 抗氧化性
Keywords:
Tetragonula gressitti (Sakagami); Lepidotrigona flavibasis (Cockerell); geopropolis; ethyl acetate; total phenols; antioxidant activity
分类号:
S896.6
DOI:
doi:10.3969/j.issn.1000-4440.2020.04.032
文献标志码:
A
摘要:
本研究以戈氏无刺蜂蜂胶和黄纹无刺蜂蜂胶为研究对象，采用超声辅助提取法，以乙酸乙脂为提取剂，比较2种蜂胶在不同料液比下的提取率，测定提取率最高条件下2种蜂胶提取物中总酚含量及其抗氧化能力。结果表明，不同料液比的提取效果不同，戈氏无刺蜂蜂胶和黄纹无刺蜂蜂胶乙酸乙酯提取物的pH范围分别为3.17～3.58和2.25～2.75；当料液比为1.0∶5.0(g/ml）时戈氏无刺蜂蜂胶和黄纹无刺蜂蜂胶具有最高的提取率，分别为47.68%±1.26%和43.12%±3.64%；总酚含量以每克样品中没食子酸当量表示，戈氏无刺蜂蜂胶和黄纹无刺蜂蜂胶乙酸乙酯提取物中的总酚含量分别为(47.91±0.36） mg/g, GAE和(58.94±1.04） mg/g, GAE，呈极显著差异(P<0.01）；DPPH 自由基清除率随2种无刺蜂蜂胶乙酸乙酯提取物的质量浓度升高而增加，半清除率质量浓度(IC50）分别为(26.30±1.04） μg/ml和(24.60±1.02） μg/ml，黄纹无刺蜂蜂胶乙酸乙酯提取物的抗氧化性高于戈氏无刺蜂蜂胶乙酸乙酯提取物。
Abstract:
In this study, the effects of different solid-liquid ratios on the extraction rate of geopropolis from Tetragonula gressitti (Sakagami) and Lepidotrigona flavibasis (Cockerell) were compared by using ultrasonic-assisted method. Total phenols content and antioxidant activity were evaluated in the ethyl acetate extracts of two stingless bee geopropolis under the condition of the highest extraction rate. The results showed that the extraction effects were different under different solid-liquid ratios of geopropolis and ethyl acetate, and pH of ethyl acetate extracts of T. gressitti and L. flavibasis ranged from 3.17 to 3.58 and 2.25 to 2.75, respectively. When the solid-liquid ratio was 1.0∶5.0 (g/ml), the highest extraction rates of T. gressitti geopropolis and L. flavibasis geopropolis were 47.68%±1.26% and 43.12%±3.64%, respectively. The total phenols content of T. gressitti (47.91±0.36) mg/g, GAE was significantly lower than that of L. flavibasis (58.94±1.04) mg/g, GAE (P<0.01). DPPH free radical scavenging capacity increased with the increasing of mass concentration of ethyl acetate extracts from two stingless bee geopropolis, and IC50 values were (26.30±1.04) μg/ml and (24.60±1.02) μg/ml, respectively, indicating those ethyl acetate extracts of L. flavibasis geopropolis had stronger antioxidant activity than those of T. gressitti geopropolis.

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备注/Memo

备注/Memo:
收稿日期：2020-02-05基金项目：海南省重点研发项目(ZDYF2018082）；国家自然科学基金项目(31402145）作者简介：吴娇(1989-），女，贵州贵阳人，博士研究生，研究方向为细胞与分子生物学。(E-mail）wujiao1202@yahoo.com。张世青为共同第一作者。通讯作者：王释婕，(E-mail）wang_yujie@yeah.net
更新日期/Last Update: 2020-09-08