[1]宋居易,刘昱辰,张苏楠,等.南美白对虾4种病原体可视化快速检测试剂盒（生物芯片法）的研发[J].江苏农业学报,2020,(03):656-665.[doi:doi:10.3969/j.issn.1000-4440.2020.03.018]
　SONG Ju-yi,LIU Yu-chen,ZHANG Su-nan,et al.Development of a rapid visualization detection kit (biochip method) for four pathogens in Penaeus vannamei[J].,2020,(03):656-665.[doi:doi:10.3969/j.issn.1000-4440.2020.03.018]
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南美白对虾4种病原体可视化快速检测试剂盒（生物芯片法）的研发()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年03期
页码:
656-665
栏目:
畜牧兽医·水产养殖
出版日期:
2020-06-30

文章信息/Info

Title:
Development of a rapid visualization detection kit (biochip method) for four pathogens in Penaeus vannamei
作者:
宋居易1; 刘昱辰2; 张苏楠2; 江高伟2; 杨华卫2
（1.江苏沿江地区农业科学研究所，江苏如皋226541；2.江苏猎阵生物科技有限公司，江苏南通226400)
Author(s):
SONG Ju-yi1; LIU Yu-chen2; ZHANG Su-nan2; JIANG Gao-wei2; YANG Hua-wei2
(1.Jiangsu Institute of Agriculture Sciences for Region along the Yangtze River, Rugao 226541, China;2.Jiangsu Huntarray Biological Technology Co., Ltd., Nantong 226400, China)
关键词:
南美白对虾; 病原体; 生物芯片法; 反向点杂交技术
Keywords:
Penaeus vannamei; pathogen; biochip method; reverse hybridization technique
分类号:
S945.4
DOI:
doi:10.3969/j.issn.1000-4440.2020.03.018
文献标志码:
A
摘要:
利用反向点杂交技术，研制灵敏度较高的南美白对虾4种病原体检测试剂盒，其检测指标包括致对虾急性肝胰腺坏死病副溶血弧菌(VpAHPND)、对虾白斑综合征病毒(WSSV)、对虾传染性皮下及造血组织坏死病毒(IHHNV)、对虾肝肠胞虫(EHP)，用重组质粒分别对其进行了最低检测限测试，并与现有推荐方法进行比较。本研究研发的检测试剂盒对4种病原体的最低检测限为1 μl 10～100 拷贝。特异性检测结果表明，4种病原体彼此不产生交叉反应，特异性良好，与各病原体推荐检测方法的结果相比，阳性符合率完全一致。本研究研发的检测试剂盒具有操作方便，灵敏度高，特异性好，检测时间短，设备要求简单等特点，适合应用于南美白对虾VpAHPND、WSSV、IHHNV、EHP的检测、筛查工作中。
Abstract:
The reverse hybridization technique was used to develop the rapid visualization detection kit (biochip method) for four pathogens in Penaeus vannamei. The detection indices included acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus (VpAHPND), white spot syndrome virus (WSSV), infectious hypodermal and hematopoietic necrosis virus (IHHNV), Enterocytozoon hepatopenaei (EHP). The recombinant plasmid was used to test the minimum detection limit, and the results based on kits were compared with those based on the common methods. The minimum detection limit of four pathogens based on this detection kit was 10-100 copies per microliter. In addition, the results of specificity tests showed that there was no cross-reaction among four pathogens. Compared with the results of the recommended detection methods, the positive coincidence rate was completely consistent. The detection kit introduced in this study has the characteristics of easy operation, high sensitivity, good specificity, short detection time and simple equipment. It is suitable for the screening and detection of VpAHPND, WSSV, IHHNV and EHP in Penaeus vannamei.

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备注/Memo

备注/Memo:
收稿日期：2019-12-13基金项目：南通市循环农业重点实验室资助项目（CP12015003）作者简介：宋居易(1989-)，女，安徽淮南人，硕士，助理研究员，主要从事农产品加工与检测研究。（E-mail）songjuyi526@163.com。刘昱辰为共同第一作者。通讯作者：杨华卫，（Tel）17701139981；（E-mail）yanghw@huntarray.com
更新日期/Last Update: 2020-07-14