[1]孙瑞萍,王峰,晁哲,等.1月龄五指山猪与长白猪骨骼肌miRNA转录组比较[J].江苏农业学报,2020,(03):620-625.[doi:doi:10.3969/j.issn.1000-4440.2020.03.013]
　SUN Rui-ping,WANG Feng,CHAO Zhe,et al.Comparative analysis on miRNA transcriptomes of skeletal muscle between one-month-old Wuzhishan pig and Landrace[J].,2020,(03):620-625.[doi:doi:10.3969/j.issn.1000-4440.2020.03.013]
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1月龄五指山猪与长白猪骨骼肌miRNA转录组比较()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年03期
页码:
620-625
栏目:
畜牧兽医·水产养殖
出版日期:
2020-06-30

文章信息/Info

Title:
Comparative analysis on miRNA transcriptomes of skeletal muscle between one-month-old Wuzhishan pig and Landrace
作者:
孙瑞萍1; 2; 王峰1; 晁哲1; 2; 刘海隆1; 2; 郑心力1; 刘圈炜1; 2; 黄丽丽1; 邢漫萍1; 魏立民1; 2
（1.海南农业科学院畜牧兽医研究所，海南海口571100；2.海南省热带动物繁育与疫病控制重点实验室，海南海口571100）
Author(s):
SUN Rui-ping1; 2; WANG Feng1; CHAO Zhe1; 2; LIU Hai-long1; 2; ZHENG Xin-li1; LIU Quan-wei1; 2; HUANG Li-li1; XING Man-ping1; WEI Li-min1; 2
(1.Institute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Science, Haikou 571100, China;2.Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Haikou 571100, China)
关键词:
五指山猪;  骨骼肌;  miRNA; 高通量测序
Keywords:
Wuzhishan pig; skeletal muscle; microRNA; high-throughput sequencing
分类号:
S828.2
DOI:
doi:10.3969/j.issn.1000-4440.2020.03.013
文献标志码:
A
摘要:
为研究microRNA(miRNA)在五指山猪和长白猪肌肉生长发育中的差异和探究差异miRNA对骨骼肌发育转录后调控的影响，以1月龄的五指山猪和长白猪背最长肌为材料，通过转录组测序和生物信息学分析筛选与骨骼肌发育相关的差异miRNA。结果显示，从2个文库中共鉴定获得318种己知的猪miRNA，五指山猪和长白猪分别鉴定出312个和306个已知miRNA，同时分别发现了83个和88个新的miRNA。获得17个差异显著的miRNA，其中16个miRNA表达上调，1个miRNA表达下调。靶基因预测和生物学功能预测发现17个差异miRNA，共预测到535个靶基因，靶向作用于mTOR信号通路和肌动蛋白细胞骨架调控的ssc-miR-486，ssc-miR-204，ssc-miR-338和ssc-miR-885-3p 4个 miRNA可能参与骨骼肌生长发育过程。
Abstract:
To investigate the differences of microRNA (miRNA) in growth and development of skeletal muscle between Wuzhishan pig and Landrace and to explore the effects of differential miRNAs on post-transcriptional regulation of skeletal muscle development, the longissimus dorsi muscles of one-month-old Wuzhishan pig and Landrace were used as materials to screen their differential miRNAs related to skeletal muscle development by transcriptome sequencing and bioinformatics analysis. A total of 318 known porcine miRNAs were identified from two libraries, 312 and 306 known miRNAs were identified in Wuzhishan and Landrace pigs respectively, and 83 and 88 new miRNAs were found at the same time. Seventeen differential miRNAs were obtained, of which 16 miRNAs were up-regulated and one miRNA was down-regulated. A total of 535 target genes were predicted by 17 differential miRNAs, targeting ssc-miR-486, ssc-miR-204, ssc-miR-338 and ssc-miR-885-3p, which were regulated by the mTOR signaling pathway and actin cytoskeleton. The four miRNAs can participate in the growth and development of skeletal muscle.

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备注/Memo

备注/Memo:
收稿日期：2019-10-14基金项目：海南省农业科学院农业科技创新专项项目（cxzx201607）；国家自然科学基金项目（31560628、31760646）；海南省省属科研院所技术开发研究专项（KYYS-2015-02）；中央引导地方科技发展专项资金 项目（ZY2019HN01）作者简介：孙瑞萍（1982-），女，山西运城人，博士，副研究员，主要研究方向为家畜遗传育种与繁殖。（E-mail）ruiping937@126.com通讯作者：魏立民，（E-mail）liminedu@126.com
更新日期/Last Update: 2020-07-14