[1]常秋燕,郭富城,冶昡青,等.牛病毒性腹泻病毒Npro蛋白的原核表达及裂解效率[J].江苏农业学报,2019,(05):1161-1166.[doi:doi:10.3969/j.issn.1000-4440.2019.05.023]
 CHANG Qiu-yan,GUO Fu-cheng,YE Xuan-qing,et al.Prokaryotic expression and splitting decomposition rate of N pro protein of bovine viral diarrhea virus[J].,2019,(05):1161-1166.[doi:doi:10.3969/j.issn.1000-4440.2019.05.023]
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牛病毒性腹泻病毒Npro蛋白的原核表达及裂解效率()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年05期
页码:
1161-1166
栏目:
畜牧兽医·水产养殖
出版日期:
2019-10-31

文章信息/Info

Title:
Prokaryotic expression and splitting decomposition rate of N pro protein of bovine viral diarrhea virus
作者:
常秋燕12郭富城12冶昡青12李凌浩12王悦萦12刘萍12苏强12马鹏12李林杰12
(1.西北民族大学生物医学研究中心,甘肃兰州730030;2.西北民族大学生命科学与工程学院,甘肃兰州730030)
Author(s):
CHANG Qiu-yan12GUO Fu-cheng12YE Xuan-qing12LI Ling-hao12WANG Yue-ying12LIU Ping12SU Qiang12MA Peng 12LI Lin-jie12JIN Li12MA Xiao-xia 12FENG Yu-ping12
(1.Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China; 2. College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China)
关键词:
牛病毒性腹泻病毒Npro蛋白质原核表达裂解效率
Keywords:
bovine viral diarrhea virus Npro protein prokaryotic expression cleavage efficiency
分类号:
S852.65+3
DOI:
doi:10.3969/j.issn.1000-4440.2019.05.023
文献标志码:
A
摘要:
为了研究牛病毒性腹泻病毒Npro蛋白的催化切割效率,首先扩增GSTZ毒株的Npro基因,克隆至原核表达载体pET-28a(+)上,使其在表达菌E.coli BL21 (DE 3)中表达,确定正确的Npro基因碱基序列,然后构建含有切割位点的pET-28a-Npro-GFP重组融合质粒,在原核表达系统中研究Npro蛋白的切割效率。结果显示,GSTZ毒株的Npro蛋白成功地在原核表达系统中表达,重组融合蛋白质Npro-GFP在原核表达系统中的切割效率约为60.28%。
Abstract:
In order to research the catalytic cleavage efficiency of bovine viral diarrhea virus Npro protein, the Npro gene in GSTZ strain was amplified and cloned into prokaryotic expression vector pET-28a (+)in E. coli BL21(DE 3) to determine the correct base sequence of Npro gene. Then the pET-28a-Npro-GFP recombinant fusion plasmid containing the authentic cleavage site was constructed. The cleavage efficiency of Npro protein was studied in prokaryotic expression system. The results showed that Npro protein of GSTZ strain was successfully expressed in prokaryotic expression system, and the cleavage efficiency of recombinant fusion protein Npro-GFP in prokaryotic expression system was about 60.28%.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2019-01-10 基金项目:2017年度甘肃省高等学科科研项目(2017B-80);兰州民海生物工程有限公司项目(XBMu-2015-Bc-11); 西北民族大学研究生科研创新项目(Yxm2016138、Yxm2018138) 作者简介:常秋燕(1991-),女,河北衡水人,硕士研究生,研究方向为病原生物学与动物疫病防治。 通讯作者:冯玉萍,(E-mail)fyp@xbmu.edu.cn;马晓霞,(E-mail)maxiaoxia956@163.com
更新日期/Last Update: 2019-11-11