[1]孙军涛,张智超,肖付刚,等.玉米芯水热预处理酶解糖化[J].江苏农业学报,2019,(03):696-700.[doi:doi:10.3969/j.issn.1000-4440.2019.03.027]
　SUN Jun-tao,ZHANG Zhi-chao,XIAO Fu-gang,et al.Enzymatic saccharification of corncob after hydrothermal pretreatment[J].,2019,(03):696-700.[doi:doi:10.3969/j.issn.1000-4440.2019.03.027]
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玉米芯水热预处理酶解糖化()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年03期
页码:
696-700
栏目:
加工贮藏·质量安全
出版日期:
2019-06-30

文章信息/Info

Title:
Enzymatic saccharification of corncob after hydrothermal pretreatment
作者:
孙军涛; 张智超; 肖付刚; 兰天璐; 詹静; 宋启伟; 胡锦辉; 郅文莉
（河南省食品安全生物标识快检技术重点实验室，许昌学院食品与生物工程学院，河南许昌461000）
Author(s):
SUN Jun-tao; ZHANG Zhi-chao; XIAO Fu-gang; LAN Tian-lu; ZHAN Jing; SONG Qi-wei; HU Jin-hui; ZHI Wen-li
(Key Laboratory of Biomarker Based Rapiddetection Technology for Food Safety of Henan Province, Food and Bioengineering College, Xuchang University, Xuchang 461000, China)
关键词:
水热预处理; 玉米芯; 木聚糖酶; 酶解糖化
Keywords:
hydrothermal pretreatment; corncob; xylanase; enzymatic sacch arification
分类号:
TS209
DOI:
doi:10.3969/j.issn.1000-4440.2019.03.027
文献标志码:
A
摘要:
优化玉米芯超微粉碎后水热预处理工艺，用扫描电镜和高效液相色谱仪分别对水热预处理后的玉米芯组织结构和酶解糖化组分进行分析。结果表明：水热预处理温度为190 ℃、时间60 min时，提取液中总糖含量最高（314 mg/g）；玉米芯水热预处理后结构呈卷曲状，随着温度升高卷曲越明显，结构越疏松，表面呈现微孔结构；水热预处理温度190 ℃、时间60 min时，提取液中葡萄糖、木二糖、木三糖和木四糖的得率分别为0001 mg/g、16740 mg/g、4306 mg/g和3164 mg/g，提取液酶解后组分中葡萄糖、木二糖、木三糖和木四糖的得率分别为4774 mg/g、64437 mg/g、6853 mg/g和1835 mg/g。
Abstract:
To optimize the hydrothermal pretreatment process of corncob after ultrafine comminution, the structure and enzymatic saccharification components of corncob after hydrothermal pretreatment were analyzed by scanning electron microscope (SEM) and high performance liquid chromatography (HPLC). The results showed that, the total sugar content in the extraction was highest (314 mg/g) when the hydrothermal pretreatment temperature was 190 ℃ and the preteratment time was 60 min. The structure of corncob was curly after hydrothermal pretreatment, the higher the temperature was, the more obvious the curl shape became, and the looser the structure and the microporous structure on the surface became. When the hydrothermal pretreatment time was bomin and temperature was 190 ℃, the yields of glucose, xylobiose, xylotriose and xylotetraose in extraction were 0001 mg/g, 16740 mg/g, 4306 mg/g and 3164 mg/g, respectively After enzymatic hydrolysis, the yields of glucose, xylobiose, xylotriose and xylotetraose in extraction were 4774 mg/g, 64437 mg/g, 6853 mg/g and 1835 mg/g, respectively.

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备注/Memo

备注/Memo:
收稿日期：2018-08-07 基金项目：农业部大宗粮食加工重点实验室开放课题（DZLS201708）；河南省科技攻关项目（162102110150）；河南省高等学校重点科研项目（17A550005）；许昌市科技计划项目 (20160212113)；许昌市基础与前沿技术研究计划项目（2019-JC-11） 作者简介：孙军涛 (1982-)，男，河南漯河人，博士，讲师，研究方向为食品科学。(E-mail) jtsfly@163.com
更新日期/Last Update: 2019-06-30