[1]吉沐祥,王晓琳,黄洁雪,等.草莓枯萎病菌拮抗细菌JX13的鉴定及生防效果评价[J].江苏农业学报,2019,(03):586-593.[doi:doi:10.3969/j.issn.1000-4440.2019.03.012]
　JI Mu-xiang,WANG Xiao-lin,HUANG Jie-xue,et al.Identification and biocontrol effects of antagonistic bacterium JX13 strain against strawberry fusarium wilt[J].,2019,(03):586-593.[doi:doi:10.3969/j.issn.1000-4440.2019.03.012]
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草莓枯萎病菌拮抗细菌JX13的鉴定及生防效果评价()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年03期
页码:
586-593
栏目:
植物保护
出版日期:
2019-06-30

文章信息/Info

Title:
Identification and biocontrol effects of antagonistic bacterium JX13 strain against strawberry fusarium wilt
作者:
吉沐祥1; 王晓琳1; 黄洁雪1; 吴祥1; 2; 陈宏州1; 杨敬辉1; 庄义庆1
（1.江苏丘陵地区镇江农业科学研究所，江苏句容212400；2.江苏省绿盾植保农药实验有限公司，江苏句容212444）
Author(s):
JI Mu-xiang1; WANG Xiao-lin1; HUANG Jie-xue1; WU Xiang1; 2; CHEN Hong-zhou1; YANG Jing-hui1; ZHUANG Yi-qing1
(1.Zhenjiang Institute of Agricultural Sciences of the NingZhen Hilly District, Jurong 212400, China;2.Jiangsu Lvdun Plant Protection Pesticide Experimentation Co., Ltd., Jurong 212444, China)
关键词:
草莓枯萎病; 拮抗细菌JX13菌株; 促生作用; 生防效果
Keywords:
strawberry fusarium wilt; antagonistic bacterium JX13 strain; growth promoting effect; biocontrol effect
分类号:
S668.4
DOI:
doi:10.3969/j.issn.1000-4440.2019.03.012
文献标志码:
A
摘要:
为明确生防菌株JX13的分类地位，评价其对草莓枯萎病的生防效果，依据JX13菌株形态、生理生化特性、16S rRNA和gyrB基因碱基序列同源性对其进行分析鉴定，采用菌丝生长速率法和田间试验评价其对草莓的促生作用和枯萎病生防效果。结果表明，JX13菌株的形态特征和生理生化特性均与芽孢杆菌很接近，16S rRNA和gyrB基因碱基序列分析发现，JX13菌株在系统发育树中与Paenibacillus polymyxa strain IIF5SWB3属于一个类群，相似性高达9900%。发酵加工的1×1010CFU/g JX13可湿性粉剂（WP）和1×1010CFU/g枯草芽孢杆菌WP对草莓枯萎病菌菌丝生长的抑制中质量浓度（EC50）分别为19977 μg/ml和41409 μg/ml。对草莓繁苗田进行2次灌根处理，JX13 WP 500倍液处理对草莓植株有明显促生作用，对草莓枯萎病的防治效果达9603%。定植当天和第7 d对定植田进行2次灌根处理，药后50 d、80 d分别进行田间调查，JX13 WP 500倍液对草莓枯萎病防治效果分别为10000%和6894%。菌株JX13被鉴定为多黏类芽孢杆菌Paenibacillus polymyxa，其发酵液加工的1×1010CFU/g WP在草莓繁苗田和定植田中具有防治枯萎病和促生的作用。
Abstract:
To identify the classification status and its effect on promoting strawberry growth and controlling fusarium wilt, JX13 strain was analyzed by phenotypic characteristics observation, physiological and biochemical indices determination, 16S rRNA and gyrB sequence assay, and the growthpromoting effect and biocontrol efficacy of JX13 on strawberry fusarium wilt were evaluated by the mycelial growth inhibition rate method and field test. The results showed that morphological, physiological and biochemical characteristics of JX13 strain were very close to those of Paenibacillus polymyxa, 16S rRNA and gyrB gene sequences suggested that JX13 strain was clustered together with Paenibacillus polymyxa strain IIF5SWB3 in phylogenetic tree, with the sequence similarity of 9900%. The EC50 values of 1×1010CFU/g JX13 WP and 1×1010CFU/g Bacillus subtilis WP against Fusarium oxysporum were 19977 μg/ml and 41409 μg/ml， respectively. Strawberry nursery was treated by twice rootirrigation, and the results indicated that JX13 WP 500 not only effectively prevented strawberry fusarium wilt (9603% control effect), but also significantly promoted seedling growth. Field investigation was conducted on 50 and 80 days after root irrigation, and the control efficacy of JX13 WP 500 to strawberry fusarium wilt was 10000% and 6894%， respectively. The JX13 strain was identified as Paenibacillus polymyxa, and 1×1010CFU/g JX13 WP had preventive efficacy to strawberry fusarium wilt and growthpromoting effect.

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备注/Memo

备注/Memo:
收稿日期：2018-09-06 基金项目：江苏现代农业产业技术体系建设项目[JATS(2018)198]；中央财政科研院所农技推广服务试点项目[TG(17)011] 作者简介：吉沐祥(1963-)，男，江苏宝应人，本科，研究员，主要从事植保农药与果树病虫害绿色防控研究开发。（E-mail）jilvdun2800@163.com 通讯作者：庄义庆，（E-mail）yqzhuang@sina.com
更新日期/Last Update: 2019-06-30