[1]刘潮,褚洪龙,韩利红,等.桑树NBSLRR类基因家族的全基因组鉴定及其调控microRNAs分析[J].江苏农业学报,2019,(03):544-553.[doi:doi:10.3969/j.issn.1000-4440.2019.03.007]
　LIU Chao,CHU Hong-long,HAN Li-hong,et al.Genomewide identification of NBSLRR genes and regulation analysis by microRNAs in mulberry[J].,2019,(03):544-553.[doi:doi:10.3969/j.issn.1000-4440.2019.03.007]
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桑树NBSLRR类基因家族的全基因组鉴定及其调控microRNAs分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年03期
页码:
544-553
栏目:
遗传育种·生理生化
出版日期:
2019-06-30

文章信息/Info

Title:
Genomewide identification of NBSLRR genes and regulation analysis by microRNAs in mulberry
作者:
刘潮; 褚洪龙; 韩利红; 杨云锦; 高永; 唐利洲
(曲靖师范学院云南高原生物资源保护与利用研究中心/生物资源与食品工程学院/云南省高校云贵高原动植物多样性及生态适应性进化重点实验室，云南 曲靖 655011)
Author(s):
LIU Chao; CHU Hong-long; HAN Li-hong; YANG Yun-jin; GAO Yong; TANG Li-zhou
(Center for Yunnan Plateau Biological Resources Protection and Utilization/College of Biological Resource and Food Engineering/Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on Yungui Plateau，Qujing Normal University，Qujing 655011， China)
关键词:
桑树; NBSLRR; 生物信息学; miRNA
Keywords:
mulberry; nucleotidebinding site leucinerich repeat(NBSLRR); bioinformatics; miRNA
分类号:
S792.99
DOI:
doi:10.3969/j.issn.1000-4440.2019.03.007
文献标志码:
A
摘要:
利用生物信息学方法，全面分析了桑树NBSLRR类基因家族组成、结构、进化、组织表达，并对该家族基因的调控microRNA（miRNA）进行了预测。共筛选到112个桑树NBSLRR类基因，根据功能域主要分为NBS、CCNBS、CCNBSLRR、NBSLRR 4种类型。内含子数和相位分析结果显示，基因结构类型多样。聚类分析结果显示不同聚类组间存在较多的类型交叉现象。该家族基因存在组织表达特异性。大部分桑树NBSLRR类基因均具有被miRNAs调控的可能性，miR472b和miR482b在调控该家族基因表达中可能发挥了主要作用。桑树NBSLRR家族基因结构和进化的复杂性决定了其功能的多样性，miRNA在控制该家族基因表达的适应性成本中发挥作用。
Abstract:
The composition, structure, evolution, tissue expression of the nucleotidebinding site (NBS) leucinerich repeat (LRR) gene family in mulberry, and the relationship between microRNA (miRNA) and the family gene were analyzed by bioinformatics. A total of 112 NBSLRR genes of mulberry were identified, which were divided into four types (NBS, CCNBS, CCNBSLRR and NBSLRR) according to functional domains. The results of intron number and phase analysis showed that the types of genetic structure were various. Cluster analysis results showed that many types gene were mixed. The family genes were specific in tissue expression. Most of the NBSLRR genes in mulberry were potential targets of miRNAs, and miR472b and miR482b played a major role in regulating the gene expression of the family. The complexity of the genetic structure and evolution of the NBSLRR family of mulberry determines the diversity of its function. miRNA may play a role in reducing the adaptive cost of gene expression.

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备注/Memo

备注/Memo:
收稿日期：2018-07-09 基金项目：国家自然科学基金项目（31460561、31760013、31860005、31860057）；云南省地方本科高校基础研究联合专项（2017FH001-034、2017FH001-037）；国家级大学生创新创业训练计划项目（201610684004、201710684012） 作者简介：刘潮（1980-），男，河北景县人，博士，副教授，研究研究方向为分子植物病理学。（E-mail）liuchao@mail.qjnu.edu.cn 通讯作者：唐利洲，（E-mail）tanglizhou@163.com
更新日期/Last Update: 2019-06-30