[1]张希瑞,高文硕,王敬国,等.吉林省水稻品种的遗传多样性及株型演化分析[J].江苏农业学报,2019,(03):497-505.[doi:doi:10.3969/j.issn.1000-4440.2019.03.001]
　ZHANG Xi-rui,GAO Wen-shuo,WANG Jing-guo,et al.Genetic diversity and plant type evolution of rice varieties in Jilin province[J].,2019,(03):497-505.[doi:doi:10.3969/j.issn.1000-4440.2019.03.001]
点击复制

吉林省水稻品种的遗传多样性及株型演化分析()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年03期
页码:
497-505
栏目:
遗传育种·生理生化
出版日期:
2019-06-30

文章信息/Info

Title:
Genetic diversity and plant type evolution of rice varieties in Jilin province
作者:
张希瑞; 高文硕; 王敬国; 邹德堂
（东北农业大学农学院，黑龙江 哈尔滨150030）
Author(s):
ZHANG Xi-rui; GAO Wen-shuo; WANG Jing-guo; ZOU De-tang
(College of Agriculture, Northeast Agriculture University, Harbin 150030, China )
关键词:
水稻; 遗传多样性; 株型
Keywords:
rice; genetic diversity; plant type
分类号:
S511
DOI:
doi:10.3969/j.issn.1000-4440.2019.03.001
文献标志码:
A
摘要:
本研究以51份吉林省不同年代的水稻品种为材料，连续2年对涉及穗、剑叶、倒二叶和倒三叶的21种株型性状进行了调查，并利用152对多态性的SSR引物，对供试品种的遗传多样性水平和不同年代品种间的遗传关系进行了分析。结果表明，多数株型性状在不同年代的品种间均存在极显著的差异（P<001）。随育成年代的推移育成品种的剑叶宽度、倒二叶宽度和倒三叶宽度逐渐增大，株高、穗抽出度、穗茎基角、剑叶基角和张角、倒二叶基角和张角以及倒三叶基角和张角逐渐缩小；弯曲穗型是吉林水稻品种的主要穗型。152对SSR引物共检测到648个等位变异，观测等位基因数、基因多样性指数、多态性信息量和Shannon信息指数的均值分别为4.263 0、0.365 4、0.556 3和1.042 2。地方品种与不同年代育成品种之间遗传差异最大。基于遗传相似系数可以将供试品种划分为2个亚群，聚类结果与它们的育成年代基本相同。
Abstract:
M342, a sulfonylurea-resistant germplasm in Brassica napus L., was developed and obtained by ethylmethane sulfonate seed treatment. According to the analysis of the cloned ALS gene sequences between M342 and herbicide-sensitive rapeseed, one single nucleic acid polymorphism found in BnALS3 of M342 led to substitution of tryptophan to leucine at site 556 of ALS, giving rise to the difference of digestion with BsrD I between ALS genes. Thus, eight primers were designed, from which a pair of primers SU54F/SU58R amplifying the targeted fragments specifically in different rapeseed In this study, 51 rice varieties of different breeding ages (VDBA) in Jilin province were used as materials, 21 plant type traits related to ear, flag leaf, the second and third leaf from the top were investigated for two consecutive years and 152 pairs polymorphic SSR primers were adopted to analyze the genetic diversity and the genetic relationships among the VDBA. The results indicated that there were highly significant difference among the most of the plant type traits which came from the VDBA in Jilin province (P<0.01). The width of flag leaf, the second and third leaf from the top gradually increased in the improved varieties. Plant height, the ear extraction, the base angle of ear, the base angle and opening angle of flag leaf, the second and third leaf from the top gradually reduced. Curved ear was the main ear type of the rice varieties in Jilin province. A total of 648 alleles were detected by 152 pairs polymorphic SSR primers. The mean values of the observed allele number, genetic diversity index, polymorphism information content and shannon information index were 4.263 0, 0.365 4, 0.556 3 and 1.042 2 respectively. The genetic difference between the landraces and the improved varieties of different breeding ages was the greatest. Based on the genetic similarity coefficient, the 51 rice varieties were divided into two subgroups. The clustering result was basically the same as their breeding ages.

参考文献/References:

[1]DALLY A M, SECOND G. Chloroplast DNA diversity in wild and cultivated species of rice (Genus Oryza, section Oryza). Cladisticmutation and geneticdistance analysis [J]. Tagtheoretical & Applied Geneticstheoretische Und Angewandte Genetik， 1990， 80(2): 209-222.
[2]FUJINO K, NISHIMURA T, KIUCHI H, et al. Phenotypic changes during 100year rice breeding programs in Hokkaido[J]. Breeding Science，2017,67(5):528-534.
[3]徐海， 宫彦龙， 夏原野，等. 中日水稻品种杂交后代的株型性状与产量和品质的关系[J].中国水稻科学， 2016， 30(3)： 283-290.
[4]易小林,陈会鲜,王彩先,等. 桂东南地区杂交水稻理想株型初探[J].南方农业学报， 2016，47(2):196-200.
[5]LEI Q, ZHOU J, ZHANG W, et al. Morphological diversity of panicle traits in Kam fragrant glutinous rice (Oryza sativa) [J]. Genetic Resources & Crop Evolution，2018,65(3): 775-786.
[6]杨静，刘海英， 金正勋，等. 黑龙江省水稻品种SSR标记遗传多样性分析[J].东北农业大学学报， 2008，39（6）:1-10.
[7]郝伟，张旭，徐正进，等. 东北三省水稻遗传多样性和亲缘关系的SSR分析[J].河南农业科学，2008（4），18-24.
[8]INTA A, BALSLEV H, GUSTAFSSON MHG, et al. Genetic diversity patterns of rice (Oryza sativa L.) landraces after migration by Tai Lue and Akha between China and Thailand[J]. Genetic Resources & Crop Evolution,2016,63(5):845-858.
[9]SAHU P K, MONDAL S, SHARMA D, et al. InDel marker based genetic differentiation and genetic diversity in traditional rice (Oryza sativa L.) landraces of Chhattisgarh, India[J]. PLoS ONE, 2017,12(11):e0188864.
[10]王敬国. 东北亚地区粳稻遗传多样性及主要株型性状与SSR标记的关联分析[D]. 哈尔滨：东北林业大学， 2014.
[11]郭元元，周生茂，陈振东，等. 广西黄瓜地方品种鉴定评价及遗传多样性分析［Ｊ］. 南方农业学报， 2018，49（7）：1273-1281.
[12]王维婷，郭溆，刘超，等. 黄秋葵种质资源结实特点遗传多样性评价[J]. 山东农业科学，2017，49(2)：26-29，35.
[13]芮文婧，张倩男，王晓敏，等. ４７份大果番茄种质资源表型性状的遗传多样性［Ｊ］. 江苏农业科学，２０１７，４５（１２）：９２-９５.
[14]曾绍贵，朱邦彤，罗木旺，等. 100 份朝天椒的农艺性状和 SRAP 标记遗传多样性分析[J].江苏农业学报，2018，34(4):871-879.
[15]余青青,王普昶,赵丽丽,等. 7 个燕麦品种的 ISSR 遗传多样性分析[J]. 江苏农业科学,2018,46(19):34-37.
[16]王少铭，侯颖辉，罗莉斯，等. 基于SRAP和ISSR标记的薄荷种质资源遗传多样性及亲缘关系分析[J]. 南方农业学报，2018，49（11）：2148-2154.
[17]徐正进，董克. 水稻叶片基角,开张角和披垂度的同时测定方法[J].沈阳农业大学学报， 1991（2）：185-187.
[18]唐启义. DPS数据处理系统-实验设计、统计分析及数据挖掘[M]. 北京：科学出版社， 2007.
[19]EDWARDS K, JOHNSTONE C, THOMPSON C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis[J]. Nucleic Acids Research, 1991,19(6):1349.
[20]TRIGIANO R, CAETANOANOLLES G. Laboratory exercises on DNA amplification fingerprinting for evaluating the molecular diversity of horticultural species[J]. Hort Technology,1998,8(6):413-423.
[21]CAETANO G, SCHLARBAUM S, Trigiano R. DNA amplification fingerprinting and marker screening for pseudotestcross mapping of flowering dogwood (Cornus florida L.)[J]. Euphytica,1999, 106(3):209-222.
[22]YEH F, YANG R, BOYLE T. Popgene Version 1.32 Microsoft Windowsbased freeware for populations genetic analysis[J]. University of Alberta, 1999,38(8):212-213.
[23]NEI M. Genetic distance between populations[J]. American Naturalist,1972,106(949):283-292.
[24]LIU K, MUSE S V. PowerMarker: an integrated analysis environment for genetic marker analysis[J]. Bioinformatics,2005,21(9):2128-2129.
[25]NEI M, TAJIMA F, TATENO Y. Accuracy of estimated phylogenetic trees from molecular data[J]. Journal of Molecular Evolution,1982,18(6):387-404.
[26]ROHLF F J. NTSYSpcnumerical taxonomy and multivariate system analysis [J]. The American Statistician, 1987, 41(4):330.
[27]PEAKALL R, SMOUSE P E. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and researchan update[J]. Bioinformatics, 2012, 28(28):2537-2539.
[28]韦还和，李超，张洪程，等.水稻甬优12不同产量群体的株型特征[J].作物学报， 2014，40（12）:2160-2168.
[29]孙成明，苏祖芳. 水稻株型的研究进展(综述) [J].上海农业学报，2004，20（1）:41-44.
[30]刘丹. 东北粳稻遗传多样性及穗部性状基因定位研究 [D]沈阳：沈阳农业大学， 2014.
[31]李红宇，侯昱铭，陈英华，等. 用SSR标记评估东北三省水稻推广品种的遗传多样性[J].中国水稻科学， 2009，23（4）:383-390.
[32]孙健，王敬国，刘化龙，等. 黑龙江省主栽水稻品种的遗传多样性分析[J].作物杂志，2011（1）:63-67.
[33]陈英华，李红宇，侯昱铭，等. 东北地区水稻种质资源遗传多样性分析[J].华北农学报，2009，24（3）:165-173.
[34]CHEN C, HE W, NASSIROU T Y, et al. Molecular characterization and genetic diversity of different genotypes of Oryza sativa and Oryza glaberrima[J]. Electronic Journal of Biotechnology, 2017,30(8):48-57.
[35]MUHAMAD K, EBANA K, FUKUOKA S, et al. Genetic relationships among improved varieties of rice (Oryza sativa L.) in Indonesia over the last 60 years as revealed by morphological traits and DNA markers[J]. Genetic Resources & Crop Evolution,2016,64(4):1-15.

相似文献/References:

[1]王士磊,丁正权,黄海祥.水稻隐性早熟突变体ref早熟性的遗传分析和基因定位[J].江苏农业学报,2016,(04):721.[doi:10.3969/j.issn.100-4440.2016.04.001]
　WANG Shi-lei,DING Zheng-quan,HUANG Hai-xiang.Inheritance and gene mapping of recessive earliness in rice (Oryza sativa L.)[J].,2016,(03):721.[doi:10.3969/j.issn.100-4440.2016.04.001]
[2]王在满,郑乐,张明华,等.不同播种方式对直播水稻倒伏指数和根系生长的影响[J].江苏农业学报,2016,(04):725.[doi:10.3969/j.issn.100-4440.2016.04.002]
　WANG Zai-man,ZHENG Le,ZHANG Ming-hua,et al.Effects of seeding manners on lodging index and root growth of directseeded rice[J].,2016,(03):725.[doi:10.3969/j.issn.100-4440.2016.04.002]
[3]易能,薛延丰,石志琦,等.微囊藻毒素对水稻种子萌发和幼苗生长的胁迫作用[J].江苏农业学报,2016,(04):729.[doi:10.3969/j.issn.100-4440.2016.04.003]
　YI Neng,XUE Yan-feng,SHI Zhi-qi,et al.Inhibitory effect of microcystins on seed germination and seedling growth of rice[J].,2016,(03):729.[doi:10.3969/j.issn.100-4440.2016.04.003]
[4]刘凯,王爱民,严国红,等.一个水稻显性矮秆突变体的遗传特性与降株高能力[J].江苏农业学报,2016,(05):968.[doi:10.3969/j.issn.1000-4440.2016.05.002]
　LIU Kai,WANG Ai-min,YAN Guo-hong,et al.Genetic analysis and plant height reduction of a dominant dwarf mutant of rice[J].,2016,(03):968.[doi:10.3969/j.issn.1000-4440.2016.05.002]
[5]王红,杨镇,裴文琪,等.功能性微生物制剂对镉胁迫下水稻生长及生理特性的影响[J].江苏农业学报,2016,(05):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
　WANG Hong,YANG Zhen,PEI Wen-qi,et al.Growth and physiological characteristics of cadmium-stressed rice influenced by functional microorganism agent[J].,2016,(03):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
[6]孙玲,单捷,毛良君,等.基于遥感和Moran's I指数的水稻面积变化空间自相关性研究[J].江苏农业学报,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.017]
　SUN Ling,SHAN Jie,MAO Liang-jun,et al.Spatial autocorrelation of changes in paddy rice area based on remote sensing and Moran’s I index[J].,2016,(03):1060.[doi:10.3969/j.issn.1000-4440.2016.05.017]
[7]张晓忆,李卫国,景元书,等.多种光谱指标构建决策树的水稻种植面积提取[J].江苏农业学报,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.018]
　ZHANG Xiao-yi,LI Wei-guo,JING Yuan-shu,et al.Extraction of paddy rice area by constructing the decision tree with multiple spectral indices[J].,2016,(03):1060.[doi:10.3969/j.issn.1000-4440.2016.05.018]
[8]裔传灯,李玮,王德荣,等.水稻GW5基因的1212-bp Indel变异对粒形的影响[J].江苏农业学报,2016,(06):1201.[doi:doi:10.3969/j.issn.1000-4440.2016.06.001]
　YI Chuan-deng,LI Wei,WANG De-rong,et al.Effect of 1212-bp Indel variation of gene GW5 on rice grain shape[J].,2016,(03):1201.[doi:doi:10.3969/j.issn.1000-4440.2016.06.001]
[9]刘红江,陈虞雯,张岳芳,等.不同播栽方式对水稻叶片光合特性及产量的影响[J].江苏农业学报,2016,(06):1206.[doi:doi:10.3969/j.issn.1000-4440.2016.06.002]
　LIU Hong-jiang,CHEN Yu-wen,ZHANG Yue-fang,et al.Effects of planting pattern on leaf photosynthetic characteristics and yield of rice[J].,2016,(03):1206.[doi:doi:10.3969/j.issn.1000-4440.2016.06.002]
[10]郭保卫,许轲,魏海燕,等.钵苗机插水稻茎秆的抗倒伏能力[J].江苏农业学报,2016,(06):1280.[doi:doi:10.3969/j.issn.1000-4440.2016.06.014]
　GUO Bao-wei,XU Ke,WEI Hai-yan,et al.Culm lodging resistance characteristics of bowl seedling mechanical-transplanting rice[J].,2016,(03):1280.[doi:doi:10.3969/j.issn.1000-4440.2016.06.014]

备注/Memo

备注/Memo:
收稿日期：2018-09-08 基金项目：国家重点研发计划项目（2017YFD0300501） 作者简介：张希瑞(1994-)，男，硕士研究生，研究方向为水稻遗传育种。（E-mail）842028064@qq.com 通讯作者：邹德堂，(E-mail)zoudt@163.com
更新日期/Last Update: 2019-06-30