[1]张巧,商飞飞,段振华,等.荸荠贮藏期间2株腐败菌鉴定及其特性[J].江苏农业学报,2019,(01):189-194.[doi:doi:10.3969/j.issn.1000-4440.2019.01.027]
 ZHANG Qiao,SHANG Fei-fei,DUAN Zhen-hua,et al.Identification and characteristics of two spoilage bacteria from water chestnut during storage[J].,2019,(01):189-194.[doi:doi:10.3969/j.issn.1000-4440.2019.01.027]
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荸荠贮藏期间2株腐败菌鉴定及其特性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年01期
页码:
189-194
栏目:
加工贮藏·质量安全
出版日期:
2019-02-26

文章信息/Info

Title:
Identification and characteristics of two spoilage bacteria from water chestnut during storage
作者:
张巧123商飞飞123段振华123唐小闲123
(1.贺州学院食品与生物工程学院,广西贺州542899;2.贺州学院食品科学与工程技术研究院,广西贺州542899;3.广西果蔬保鲜和深加工研究人才小高地,广西贺州542899)
Author(s):
ZHANG Qiao123SHANG Fei-fei123DUAN Zhen-hua123TANG Xiao-xian123
(1.College of Food and Biological Engineer, Hezhou University, Hezhou 542899, China;2.Institute of Food Science and Engineering Research, Hezhou University, Hezhou 542899, China;3.Guangxi Talent Highland for Preservation and Deep Processing Research of Fruits and Vegetables, Hezhou 542899, China)
关键词:
荸荠贮藏腐败菌鉴定
Keywords:
water chestnutstoragespoilage bacteriaidentification
分类号:
S645.3
DOI:
doi:10.3969/j.issn.1000-4440.2019.01.027
文献标志码:
A
摘要:
为确定引起荸荠腐烂的细菌种类及其主要生物学特性,从腐烂的荸荠中分离到2株细菌,经16S RNA基因序列结果分析,确定其分别为木糖氧化无色杆菌(Achromobacter xylosoxidans)和产气肠杆菌(Enterobacter aerogenes)。对其生物学特性进行研究后发现,2株腐败菌生长速度较快;产气肠杆菌的耐酸、耐温、耐盐性较强,培养液初始pH 3.5,培养温度 45 ℃,或培养液中添加7.5%的NaCl,仍不能完全抑制其生长,而木糖氧化无色杆菌的耐酸、耐温、耐盐性则较差;此外,2株菌株对青霉素、红霉素、链霉素及氯霉素的抗性较强,最低抑制质量浓度均高于1 024 μg/ml,对庆大霉素、万古霉素及四环素的最低抑制质量浓度均低于1 024 μg/ml,且产气肠杆菌的耐受力强于木糖氧化无色杆菌。本研究结果为荸荠贮藏过程中的抗菌防腐提供了理论依据。
Abstract:
In order to learn about the species and characteristics of bacteria which cause the corruption of water chestnut, two bacteria were isolated from the rotten water chestnut, and then they were identified as Achromobacter xylosoxidans and Enterobacter aerogenes by 16S RNA sequence analysis. Results of biological characteristics showed that two strains of spoilage bacteria had fast growth rate. E. aerogenes grew slightly under the condition of initial pH3.5, culture temperature 45 ℃ or 7.5% NaCl addition, while for A. xylosoxidans, the acid, temperature and NaCl endurance were weaker. In addition, two spoilage bacteria presented strong resistance to ampicillin, erythromycin, streptomycin and chloramphenicol, and the minimum inhibitory concentration (MIC) was higher than 1 024 μg/ml. However, MIC for gentamicin, vancomycin and tetracycline was less than 1 024 μg/ml, and E. aerogenes showed stronger resistance than A. xylosoxidans. These results provide theoretical basis for antimicrobial and antiseptic storage of water chestnut.

参考文献/References:

[1]张怡,胡月珍,李小根.荸荠营养成分及保健价值的研究[J].科技创业家,2013(3):198.
[2]刘兵,段振华.马蹄的贮藏保鲜与加工利用研究进展[J].食品研究与开发,2016,37(18):212-215.
[3]PAN Y G, LI Y X, YUAN M Q. Isolation, purification and identification of etiolation substrate from fresh-cut Chinese water chestnut (Eleocharis tuberosa) [J]. Food Chemistry, 2015, 186:119-122.
[4]尹璐,彭勇,梅俊,等.不同涂膜保鲜处理对荸荠品质变化的影响[J].食品科学,2013,34(20):297-301.
[5]YOU Y L,JIANG Y M,SUN J,et al. Effects of short-term anoxia treatment on browning of fresh-cut Chinese water chestnut in relation to antioxidant activity[J].Food Chemistry, 2012, 132 (3):1191-1196.
[6]王强.荸荠贮藏保鲜的六种方法[J].农家顾问,2011(1):50.
[7]鲍建荣,詹有才,吴学素,等.荸荠茎腐病病原菌鉴定及生物学特性的研究[J].植物保护学报,1993,20(4):307-311.
[8]颜梅新,袁高庆,陈世伟,等.荸荠贮藏期真菌性病害种类调查鉴定初探[J].广西农业生物科学,2003,22(2):96-99.
[9]颜梅新,廖旺姣,袁高庆,等.广西荸荠贮藏期腐烂病病原菌鉴定[J].广西农业科学,2009,40(7):840-843.
[10]田伟,张琦,邓珍珍,等.利用16S rRNA分析传统四川发酵泡菜中的细菌多样性[J].食品科学,2013,34(17):215-218.
[11]PERES C M, ALVES M, HERNANDEZ- MENDOZA A, et al. Novel isolates of lactobacilli from fermented Portuguese olive as potential probiotics[J]. LWT- Food Science and technology, 2014, 59:234-246.
[12]梁亮.木糖氧化无色杆菌研究进展[J].右江医学,2017,45(1):112-115.
[13]叶罗娜.鸡腿菇菌柄腐烂病病原鉴定与防治技术初探[D].武汉:华中农业大学,2017.
[14]高磊,谢晶,叶藻,等.冷鲜鸡腿肉中优势腐败菌的分离机腐败能力研究[J].食品与发酵工业,2015,41(8):48-53.
[15]戚峰.生物质高效水解及发酵产氢的机理研究[D].杭州:浙江大学,2007.
[16]刘丽莎,彭义交,鲍鲁生,等.大豆浸泡过程中腐败微生物对豆浆品质的影响[J].食品科学,2015,36(14):161-164.
[17]郭全友,何木,李保国,等.大黄鱼腐败细菌鉴定及PLFAs初级模型的建立[J].现代食品科技,2015,31(12):227-234.
[18]董湘熔. 吲哚醌和邻氨基苯甲酸降解菌株的选育及降解性能研究[D].郑州:郑州大学,2011.
[19]张全国,赫倚风,胡建军,等.产气肠杆菌厌氧发酵产氢工艺实验[J].农业机械学报,2014,45(2):176-180.

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备注/Memo

备注/Memo:
收稿日期:2018-10-29 基金项目:广西高校中青年教师基础能力提升项目(2018KY0557);贺州学院食品学科与工程广西一流学科培育项目(GXYLXKP1825) 作者简介:张巧(1990-),女,湖南衡阳人,硕士,助教,主要从事农产品贮藏保鲜和深加工研究。(Email)zq2203@126.com
更新日期/Last Update: 2019-02-27