[1]陈静,何吉祥,樊佳佳,等.草鱼MyoD基因SNP和Indel标记的筛选及其与生长性状的关联分析[J].江苏农业学报,2018,(03):612-616.[doi:doi:10.3969/j.issn.1000-4440.2018.03.019]
　CHEN Jing,HE Ji-xiang,FAN Jia-jia,et al.Screening of SNP and Indel marker of MyoD gene and its association with growth traits in Ctenopharyngodon idella[J].,2018,(03):612-616.[doi:doi:10.3969/j.issn.1000-4440.2018.03.019]
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草鱼MyoD基因SNP和Indel标记的筛选及其与生长性状的关联分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年03期
页码:
612-616
栏目:
畜牧兽医·水产养殖
出版日期:
2018-06-25

文章信息/Info

Title:
Screening of SNP and Indel marker of MyoD gene and its association with growth traits in Ctenopharyngodon idella
作者:
陈静1; 何吉祥1; 樊佳佳2; 黄龙1; 吴本丽1; 宋光同1; 汪翔1; 武松1
（1.安徽省农业科学院水产研究所，安徽合肥230031；2.农业部热带亚热带水产资源利用与养殖重点实验室，广东广州510380）
Author(s):
CHEN Jing1; HE Ji-xiang1; FAN Jia-jia2; HUANG Long1; WU Ben-li1; SONG Guang-tong1;  WANG Xiang1; WU Song1
(1.Fishery Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China;2.Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Guangzhou 510380, China)
关键词:
草鱼; MyoD基因; SNP标记; Indel标记; 生长性状
Keywords:
Ctenopharyngodon idella; MyoD; SNP marker; Indel marker; growth trait
分类号:
S965.112
DOI:
doi:10.3969/j.issn.1000-4440.2018.03.019
文献标志码:
A
摘要:
为探索MyoD基因多态性对草鱼(Ctenopharyngodon idella)生长性状的影响，采用PCR产物直接测序的方法，检测草鱼MyoD基因上的突变位点，并分析其与草鱼生长性状的相关性。结果表明：在草鱼MyoD基因上筛选到4个多态性高、分型稳定的突变位点，即M1（940后T碱基的插入）、M3（1630后重复单元AATAGCCT的缺失）、M4（G1669A）和M5（A1681T）。M1位点不同基因型个体间的体长、全长、尾柄长和尾柄高存在显著差异（P<0.05），纯合缺失基因型（DD）显著大于纯合插入基因型（TT）；M3和M4位点不同基因型个体间的生长性状差异均不显著（P>0.05）；M5位点不同基因型个体间的体质量、体宽、体高和眼间距存在显著差异（P<0.05），优势基因型为TT。MyoD基因的M1和M5突变位点可作为草鱼选育的候选分子标记。
Abstract:
To explore the effect of MyoD gene polymorphism on grass carp(Ctenopharyngodon idella) growth traits, mutation sites of MyoD in grass carp were determined by PCR production sequencing, and the association between genotypes and growth traits was investigated. The results showed that four mutation sites which had high polymorphism and stable typing were found in MyoD gene of grass carp, M1(insertion of T base after 940), M3(deletion of repeated unit of AATAGCCT after 1630), M4(G1669A) and M5(A1681T), respectively. There were significant differences in body length, total length, caudal peduncle length and caudal peduncle height of M1 trait of different genotypes (P<0.05), homozygous deletion genotype (DD) was significantly higher than homozygous insertion genotype (TT). There was no significant difference in the growth traits between individuals of M3 and M4 traits(P>0.05). There were significant differences in body weight, body width, body height and interocular distance among individuals with different genotypes at M5 trait(P<0.05), and the dominant genotype was TT. The M1 and M5 mutation sites of MyoD gene can be used as candidate molecular markers for selection of grass carp.

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备注/Memo

备注/Memo:
收稿日期：2017-11-28 基金项目：安徽省农业科学院学科建设项目(15A0516)；安徽省农业科学院科技创新团队建设项目（13C0506）；安徽省水产产业技术体系项目[皖农科2016(84)号] 作者简介：陈静（1985-），女，山东单县人，硕士，助理研究员，主要从事水产动物遗传育种研究。（E-mail）chenjingok5@163.com 通讯作者：何吉祥，（E-mail）hejixiang813@126.com
更新日期/Last Update: 2018-07-04