[1]李惠根,王晓宇,李平,等.蓖麻液泡膜质子泵RcVP1基因克隆与表达分析[J].江苏农业学报,2018,(02):251-258.[doi:doi:10.3969/j.issn.1000-4440.2018.02.003]
　LI Hui-gen,WANG Xiao-yu,LI Ping,et al.Cloning and expression analysis of vacuolar membrane proton pump RcVP1 gene from castor (Ricinus communis L.)[J].,2018,(02):251-258.[doi:doi:10.3969/j.issn.1000-4440.2018.02.003]
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蓖麻液泡膜质子泵RcVP1基因克隆与表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年02期
页码:
251-258
栏目:
遗传育种·生理生化
出版日期:
2018-04-25

文章信息/Info

Title:
Cloning and expression analysis of vacuolar membrane proton pump RcVP1 gene from castor (Ricinus communis L.)
作者:
李惠根1; 2; 王晓宇1; 2; 李平1; 2; 从娇娇1; 2; 张丽雪1; 2; 张继星1; 2; 冀照君1; 2; 穆莎茉莉1
(1.内蒙古民族大学生命科学学院/内蒙古自治区高校蓖麻产业工程技术研究中心/内蒙古自治区蓖麻育种重点实验室/内蒙古自治区蓖麻产业协同创新培育中心, 内蒙古通辽028000;2.内蒙古民族大学科尔沁植物逆境生物学研究所, 内蒙古通辽028000)
Author(s):
LI Hui-gen1; 2; WANG Xiao-yu1; 2; LI Ping1; 2; CONG Jiao-jiao1; 2; ZHANG Li-xue1; 2; ZHANG Ji-xing1; 2; JI Zhao-jun1; 2; MU Sha-moli1
(1.College of Life Science, Inner Mongolia University for the Nationalities/Inner Mongolia Industrial Engineering Research Center of Universities for Castor/Inner Mongolia Key Laboratory of Castor Breeding/Inner Mongolia Collaborative Innovation Cultivate Center for Castor, Tongliao 028000, China;2.Horqin Plant Stress Biology Research Institute of Inner Mongolia University for Nationalities, Tongliao 028000, China)
关键词:
蓖麻; 液泡膜质子泵; 基因克隆; 表达分析
Keywords:
Ricinus communis L.; vacuolar membrane proton pump; gene cloning; expression analysis
分类号:
S565.601
DOI:
doi:10.3969/j.issn.1000-4440.2018.02.003
文献标志码:
A
摘要:
为挖掘耐盐基因，培育耐盐蓖麻新种质，本研究设计特异性引物克隆蓖麻液泡膜H+-PPase基因，将其命名为RcVP1，该基因开放阅读框为2 304 bp，编码767个氨基酸，其编码蛋白质的分子量和等电点分别为8.04×104和4.94。RcVP1含有保守的H+-PPase结构域，包括CS1、CS2、CS3高度保守区，有13个跨膜结构域。多重序列比对结果表明RcVP1氨基酸序列与毛白杨的相似性最高，达95.00 %，与Ⅰ型H+-PPase拟南芥AVP1的相似性高达88.53 %，与Ⅱ型H+-PPase 拟南芥AVP2的相似性只有36.41 %，属于Ⅰ型液泡膜H+-PPase跨膜蛋白质。半定量PCR分析结果表明，该基因在蓖麻叶片、茎中受盐胁迫诱导上调表达，在种子中抑制表达。
Abstract:
In order to explore the salt-tolerant genes and cultivate a new germplasm of salt-tolerant castor, the specific primers were designed to clone the H+-PPase gene , named RcVP1. The open reading frame(ORF) of RcVP1 gene was 2 304 bp and encoded 767 amino acids. The molecular weight and isoelectric point of the encoded protein were 8.04×104 and 4.94, respectively. RcVP1 contained three conserved H+-PPase domains (CS1, CS2, CS3), and it had 13 transmembrane domains. Multiple alignment results showed that the amino acid sequence of RcVP1 had 95.00% and 88.53% similarities with that of Populus tomentosa H+-PPase and Arabidopsis thaliana type I H+-PPase AVP1, respectively. However, it only had 36.41% similarities with that of Arabidopsis thaliana typeⅡH+-PPase AVP2. Therefore RcVP1 belonged to type I vacuolar membrane proton pump transmembrane protein. Semi-quantitative PCR analysis results displayed that RcVP1 gene up-regulated in the leaves and stems, down-regulated in the seeds under salt stress.

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备注/Memo

备注/Memo:
收稿日期：2017-09-18 基金项目：国家自然科学基金项目(31260336、31760399、31701467)；内蒙古自治区蓖麻育种重点实验室开放基金项目(MDK2017036、MDK2017037) 作者简介：李惠根(1993-), 女,内蒙古通辽人, 硕士研究生, 主要从事植物抗逆分子生物学研究。(Tel) 14747156686; (E-mail) xiaogener369@hotmail.com 通讯作者：张继星, (E-mail) zhangjixing@imun.edu.cn
更新日期/Last Update: 2018-05-04