[1]徐微风,覃和业,刘姣,等.冰菜在不同浓度海水胁迫下的氧化胁迫和抗氧化酶活性变化[J].江苏农业学报,2017,(04):775-781.[doi:doi:10.3969/j.issn.1000-4440.2017.04.008]
　XU Wei-feng,QIN He-ye,LIU Jiao,et al.Changes of oxidative stress and antioxidant enzyme activity of Mesembryanthemum crystallinum Linnaeus in response to different concentrations of seawater[J].,2017,(04):775-781.[doi:doi:10.3969/j.issn.1000-4440.2017.04.008]
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冰菜在不同浓度海水胁迫下的氧化胁迫和抗氧化酶活性变化()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年04期
页码:
775-781
栏目:
遗传育种·生理生化
出版日期:
2017-08-30

文章信息/Info

Title:
Changes of oxidative stress and antioxidant enzyme activity of Mesembryanthemum crystallinum Linnaeus in response to different concentrations of seawater
作者:
徐微风1; 2; 覃和业2; 刘姣2; 王蕾1; 2; 吕瑞1; 2; 郭建春2; 符少萍2; 李瑞梅2; 胡新文1; 段瑞军2
(1.海南大学,海南海口571101;2.中国热带农业科学院热带生物技术研究所,海南海口571101)
Author(s):
XU Wei-feng1; 2; QIN He-ye2; LIU Jiao2; WANG Lei1; 2; LYU Rui1; 2; GUO Jian-chun2; FU Shao-ping2; LI Rui-mei2; HU Xin-wen1; DUAN Rui-jun2
(1.Hainan University, Haikou 571101, China;2.Institute of Tropical Bioscience and Biotechnology, China Academy of Tropical Agricultural Science, Haikou 571101, China)
关键词:
冰菜; 海水胁迫; 氧化胁迫; 抗氧化酶活性
Keywords:
Mesembryanthemum crystallinum Linnaeus; seawater stress; oxidative stress; antioxidant enzyme activity
分类号:
Q945.78
DOI:
doi:10.3969/j.issn.1000-4440.2017.04.008
文献标志码:
A
摘要:
通过分析冰菜（Mesembryanthemum crystallinum Linnaeus）在不同海水浓度的灌溉水栽培条件下受到的氧化胁迫程度，进一步分析其抗氧化酶活性变化，初步探索抗氧化途径在冰菜耐盐机制中的作用。本研究以冰菜为材料，通过盆栽试验，设置灌溉水中6个海水浓度处理，分别为0、2000%、4000%、6000%、8000%和10000%，测定不同处理下冰菜在150 d内的生长形态、氧化胁迫程度和抗氧化酶活性的变化，每隔30 d测一次。与0海水浓度处理相比，小于等于6000%海水浓度处理下的冰菜未表现出生长抑制，冰菜体内H2O2含量和O2·-含量分别小于1000 μmol/g和015 μmol/g，丙二醛(MDA)含量和叶片相对电导率分别小于2700 μmol/g和5370%。小于6000%海水浓度处理下，超氧化物歧化酶(SOD)活性随着冰菜的生长呈递增趋势，过氧化物酶(POD)活性随着冰菜的生长呈先升高后降低再升高的趋势，过氧化氢酶(CAT)活性低于100 U/g，2000%海水浓度处理下的冰菜得到最显著正向生长刺激。大于6000%海水浓度处理下的冰菜表现出与海水浓度正相关的生长抑制，其体内H2O2含量和O2·-含量激增，MDA含量和叶片电导率随冰菜生长衰老呈持续递增趋势，SOD活性和POD活性均随着冰菜生长呈递减趋势。8000%海水浓度处理下CAT活性变化不明显，保持在100 U/g左右，10000%海水浓度处理下CAT活性激增。冰菜能在全海水浇灌环境中生长，最适合其生长的海水浓度为2000%，当海水处理浓度小于等于6000%时，其抗逆性先增后减，当海水处理浓度大于6000%时，其抗逆性逐渐减弱。海水胁迫对冰菜产生了氧化胁迫，在SOD、POD和CAT抗氧化酶促系统中，与氧化胁迫相关性最强的是SOD，CAT的激活需要冰菜体内H2O2含量和O2·-含量达到一定水平。
Abstract:
A pot experiment was conducted by treating Mesembryanthemum crystallinum L. with six concentrations of seawater （0, 2000%, 4000%, 6000%, 8000% and 10000%, respectively） to analyze the growth pattern, oxidative stress and antioxidant enzyme activity every 30 d. Compared with no seawater treatment, growth was not inhibited in the treatments with seawater concentrations less than 60%, the contents of H2O2 and O2·- were less than 1000 μmol/g and 015 μmol/g, and the contents of malondialdehyde (MDA) and relative electric conductivities were less than 2700 μmol/mg and 5370%, respectively. At the same time, the activity of superoxide dismutase (SOD) increased over growth period, and peroxidase (POD) activity increased twice. The activity of catalase (CAT) was less than 100 U/g. The most obvious positive growth stimulation was observed in 2000% seawater treatment. When seawater concentration was more than 6000% , positively related growth inhibition was observsed, which were shown as sharply increased contents of H2O2 and O2·- in M. crystallinum L., increased MDA and relative conductivities, and decreased activities of SOD and POD. CAT activity kept stable in 8000% seawater treatment, but increased sharply in 10000% treatment. The results indicated that M. crystallinum L. could grow under whole seawater irrigation and 2000% concentration was the optimum. When seawater concentration was more than 6000%, stress resistance decreased gradually. Sea water stress induced oxidative stress.

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备注/Memo

备注/Memo:
收稿日期：2017-03-21 基金项目：中国科协青年人才托举工程项目(YESS20150167)；中国热带农业科学院资助项目（1630012017009）；中国热带农业科学院热带生物技术研究所资助项目（1630052016011） 作者简介：徐微风（1992-），女，安徽安庆人，硕士研究生，主要从事植物抗逆研究。（E-mail）708992254@qq.com 通讯作者：段瑞军，（E-mail）lshjz6@163.com
更新日期/Last Update: 2017-09-01