[1]裔传灯,李玮,王德荣,等.水稻GW5基因的1212-bp Indel变异对粒形的影响[J].江苏农业学报,2016,(06):1201-1205.[doi:doi:10.3969/j.issn.1000-4440.2016.06.001]
　YI Chuan-deng,LI Wei,WANG De-rong,et al.Effect of 1212-bp Indel variation of gene GW5 on rice grain shape[J].,2016,(06):1201-1205.[doi:doi:10.3969/j.issn.1000-4440.2016.06.001]
点击复制

水稻GW5基因的1212-bp Indel变异对粒形的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年06期
页码:
1201-1205
栏目:
遗传育种·生理生化
出版日期:
2017-02-07

文章信息/Info

Title:
Effect of 1212-bp Indel variation of gene GW5 on rice grain shape
作者:
裔传灯; 李玮; 王德荣; 蒋伟; 王颖; 周勇; 梁国华; 顾铭洪
（扬州大学江苏省作物遗传生理国家重点实验室培育点/粮食作物现代产业技术协同创新中心，教育部植物功能基因组学重点实验室,江苏扬州225009）
Author(s):
YI Chuan-deng; LI Wei; WANG De-rong; JIANG Wei; WANG Ying; ZHOU Yong; LIANG Guo-hua; GU Ming-hong
(Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center forModern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China)
关键词:
水稻; 粒形; GW5基因; 插入缺失变异
Keywords:
rice; grain shape; gene GW5; indel variation
分类号:
S511.035.3
DOI:
doi:10.3969/j.issn.1000-4440.2016.06.001
文献标志码:
A
摘要:
为了探明粒宽基因GW5对水稻粒形的作用，本研究利用DNA序列分析技术和PCR技术，对已测序的稻属野生种、水稻微核心种质以及2007-2013年江苏省审定的粳稻品种中GW5的变异类型进行了鉴定。研究结果表明，野生稻都含有GW5基因的1212-bp序列，但DNA序列有着明显的分化。在水稻微核心种质的籼粳亚种中，相对于GW5插入型(Ins)而言，缺失型(Del）水稻品种有着较短的粒长、较宽的粒宽、较厚的粒厚、较小的长宽比和较重的千粒质量，并且GW5基因的变异对籼亚种的粒形和千粒质量的影响均达到极显著水平。江苏省近年审定的绝大部分粳稻品种中GW5基因为缺失型。GW5基因的1212-bp Indel变异对水稻粒形和千粒质量有着不同程度的影响，这些研究结果对利用GW5的等位基因进行水稻育种有着重要的指导价值。
Abstract:
To explore the effect of grain width gene GW5 on rice grain shape, the analysis of genomic DNA sequence and PCR were conducted in sequenced wild species of genus Oryza, rice mini core collection (RMCC) and certificated japonica varieties in Jiangsu province from 2007 to 2013. We found that GW5 involved 1212-bp seuqence in the wild rices. The phylogeny demonstrated an apparent differentiation on DNA sequence of GW5 among these species. In the RMCC, rice varieties with the Del genotype of GW5 had shorter grain length, wider grain width, thicker grain thickness, smaller ratio of length to width and larger 1000-grain weight, as compared with the ones of the Ins genotype. And very significant differences in the grain shape and 1000-grain weight were found in the Indica subspecies. Subsequently, the rice of Del genotype of GW5 was detected in the vast majority of japonica varieties certificated in Jiangsu province. There are different effects of GW5 related 1212-bp Indel allelic variation on rice grain shape and 1000-grain weight, which will pay the way to utilize the allelic variations of gene GW5 in the rice yield breeding program.

参考文献/References:

［1］WANG S, LI S, LIU Q, et al. The OsSPL16-GW7 regulatory module determines grain shape and simultaneously improves rice yield and grain quality [J]. Nature Genetics, 2015, 47(8):949-954.
[2]蔡永盛，郑桂萍，奚浩然，等． 高垩白与低垩白水稻穗部性状及产量的比较［J］． 江苏农业科学，2014，42(12):79-82．
[3]XING Y, ZHANG Q. Genetic and molecular bases of rice yield [J]. The Annual Review of Plant Biology, 2010, 61:421-442.
[4]陈静． 江苏省水稻食味改良育种研究进展［J］． 江苏农业科学，2015，43(12):77-80．
[5]HUANG R, JIANG L, ZHENG J, et al. Genetic bases of rice grain shape: so many genes, so little known [J]. Trends in Plant Science, 2013, 18(4):218-226.
[6]丁丹，张亚东，郑佳，等． 水稻粒长基因GS3和qGL3功能标记的设计及应用［J］． 江苏农业学报， 2014， 30(6):1191-1197.
[7]WANG S, WU K, YUAN Q, et al. Control of grain size, shape and quality by OsSPL16 in rice [J]. Nature Genetics, 2012, 44(8):950-954.
[8]FAN C, XING Y, MAO H, et al. GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein [J]. Theoretical and Applied Genetics, 2006, 112(6):1164-1171.
[9]ZHOU Y, ZHU J, LI Z, et al. Deletion in a quantitative trait gene qPE9-1 associated with panicle erectness improves plant architecture during rice domestication [J]. Genetics, 2009, 183(1):315-324.
[10]ZHANG X, WANG J, HUANG J, et al. Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice [J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(52):21534-21539.
[11]QI P, LIN Y S, SONG X J, et al. The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1;3 [J]. Cell Research, 2012, 22(12):1666-1680.
[12]ZHOU Y, MIAO J, GU H, et al. Natural variations in SLG7 regulate grain shape in rice [J]. Genetics, 2015, 201(4):1591-1599.
［13］ISHIMARU K, HIROTSU N, MADOKA Y, et al. Loss of function of the IAA-glucose hydrolase gene TGW6 enhances rice grain weight and increases yield [J]. Nature Genetics, 2013, 45(6):707-711.
［14］SONG X J, HUANG W, SHI M, et al. A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase [J]. Nature Genetics, 2007, 39(5):623-630.
［15］SHOMURA A, IZAWA T, EBANA K, et al. Deletion in a gene associated with grain size increased yields during rice domestication [J]. Nature Genetics, 2008, 40(8):1023-1028.
［16］WENG J, GU S, WAN X, et al. Isolation and initial characterization of GW5, a major QTL associated with rice grain width and weight [J]. Cell Res, 2008, 18(12):1199-1209.
［17］LI Y, FAN C, XING Y, et al. Natural variation in GS5 plays an important role in regulating grain size and yield in rice [J]. Nature Genetics, 2011, 43(12):1266-1269.
［18］SUN L, LI X, FU Y, et al. GS6, a member of the GRAS gene family, negatively regulates grain size in rice [J]. Journal of Integrative Plant Biology, 2013, 55(10):938-949.
［19］王军,杨杰,徐祥,等. 水稻千粒重基因TGW6功能标记的开发与利用[J]. 中国水稻科学, 2014, 28(5):473-478.
［20］ZHANG H, ZHANG D, WANG M, et al. A core collection and mini core collection of Oryza sativa L. in China [J]. Theoretical and Applied Genetics, 2011, 122(1):49-61.
［21］韩龙植, 魏兴华. 水稻种质资源描述规范和数据标准[M].北京：中国农业出版社, 2006:18-19.
［22］TAPIA-TUSSELL R, QUIJANO-RAMAYO A, ROJAS-HERRERA R, et al. A fast, simple, and reliable high-yielding method for DNA extraction from different plant species [J]. Molecular Biotechnology, 2005, 31(2):137-139.
［23］JIANG L, LIU L. New evidence for the origins of sedentism and rice domestication in the Lower Yangzi River, China [J]. Antiquity, 2006, 80:355-361.
［24］WANG M, YU Y, HABERER G, et al. The genome sequence of African rice(Oryza glaberrima) and evidence for independent domestication [J]. Nature Genetics, 2014, 46(9):982-988.
［25］DUAN S, LU B, LI Z, et al. Phylogenetic analysis of AA-genome Oryza species (Poaceae) based on chloroplast, mitochondrial, and nuclear DNA sequences [J]. Biochemical Genetics, 2007, 45(1/2):113-129.
［26］ZHU T, XU P Z, LIU J P, et al. Phylogenetic relationships and genome divergence among the AA- genome species of the genus Oryza as revealed by 53 nuclear genes and 16 intergenic regions [J]. Molecular Phylogenetics and Evolution, 2014, 70:348-361.

相似文献/References:

[1]王士磊,丁正权,黄海祥.水稻隐性早熟突变体ref早熟性的遗传分析和基因定位[J].江苏农业学报,2016,(04):721.[doi:10.3969/j.issn.100-4440.2016.04.001]
　WANG Shi-lei,DING Zheng-quan,HUANG Hai-xiang.Inheritance and gene mapping of recessive earliness in rice (Oryza sativa L.)[J].,2016,(06):721.[doi:10.3969/j.issn.100-4440.2016.04.001]
[2]王在满,郑乐,张明华,等.不同播种方式对直播水稻倒伏指数和根系生长的影响[J].江苏农业学报,2016,(04):725.[doi:10.3969/j.issn.100-4440.2016.04.002]
　WANG Zai-man,ZHENG Le,ZHANG Ming-hua,et al.Effects of seeding manners on lodging index and root growth of directseeded rice[J].,2016,(06):725.[doi:10.3969/j.issn.100-4440.2016.04.002]
[3]易能,薛延丰,石志琦,等.微囊藻毒素对水稻种子萌发和幼苗生长的胁迫作用[J].江苏农业学报,2016,(04):729.[doi:10.3969/j.issn.100-4440.2016.04.003]
　YI Neng,XUE Yan-feng,SHI Zhi-qi,et al.Inhibitory effect of microcystins on seed germination and seedling growth of rice[J].,2016,(06):729.[doi:10.3969/j.issn.100-4440.2016.04.003]
[4]刘凯,王爱民,严国红,等.一个水稻显性矮秆突变体的遗传特性与降株高能力[J].江苏农业学报,2016,(05):968.[doi:10.3969/j.issn.1000-4440.2016.05.002]
　LIU Kai,WANG Ai-min,YAN Guo-hong,et al.Genetic analysis and plant height reduction of a dominant dwarf mutant of rice[J].,2016,(06):968.[doi:10.3969/j.issn.1000-4440.2016.05.002]
[5]王红,杨镇,裴文琪,等.功能性微生物制剂对镉胁迫下水稻生长及生理特性的影响[J].江苏农业学报,2016,(05):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
　WANG Hong,YANG Zhen,PEI Wen-qi,et al.Growth and physiological characteristics of cadmium-stressed rice influenced by functional microorganism agent[J].,2016,(06):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
[6]孙玲,单捷,毛良君,等.基于遥感和Moran's I指数的水稻面积变化空间自相关性研究[J].江苏农业学报,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.017]
　SUN Ling,SHAN Jie,MAO Liang-jun,et al.Spatial autocorrelation of changes in paddy rice area based on remote sensing and Moran’s I index[J].,2016,(06):1060.[doi:10.3969/j.issn.1000-4440.2016.05.017]
[7]张晓忆,李卫国,景元书,等.多种光谱指标构建决策树的水稻种植面积提取[J].江苏农业学报,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.018]
　ZHANG Xiao-yi,LI Wei-guo,JING Yuan-shu,et al.Extraction of paddy rice area by constructing the decision tree with multiple spectral indices[J].,2016,(06):1060.[doi:10.3969/j.issn.1000-4440.2016.05.018]
[8]刘红江,陈虞雯,张岳芳,等.不同播栽方式对水稻叶片光合特性及产量的影响[J].江苏农业学报,2016,(06):1206.[doi:doi:10.3969/j.issn.1000-4440.2016.06.002]
　LIU Hong-jiang,CHEN Yu-wen,ZHANG Yue-fang,et al.Effects of planting pattern on leaf photosynthetic characteristics and yield of rice[J].,2016,(06):1206.[doi:doi:10.3969/j.issn.1000-4440.2016.06.002]
[9]郭保卫,许轲,魏海燕,等.钵苗机插水稻茎秆的抗倒伏能力[J].江苏农业学报,2016,(06):1280.[doi:doi:10.3969/j.issn.1000-4440.2016.06.014]
　GUO Bao-wei,XU Ke,WEI Hai-yan,et al.Culm lodging resistance characteristics of bowl seedling mechanical-transplanting rice[J].,2016,(06):1280.[doi:doi:10.3969/j.issn.1000-4440.2016.06.014]
[10]董海霞,赵明柳,唐守寅,等.石灰对土壤中Cd 和Zn 形态及对水稻有效性的影响[J].江苏农业学报,2016,(06):1320.[doi:doi:10.3969/j.issn.1000-4440.2016.06.020]
　DONG Hai-xia,ZHAO Ming-liu,TANG Shou-yin,et al.The effects of liming on the fraction and bioavailability to rice of Cd and Zn in a contaminated soil[J].,2016,(06):1320.[doi:doi:10.3969/j.issn.1000-4440.2016.06.020]
[11]方先文,张云辉,肖西林,等.基于重组自交系群体的水稻粒形QTL定位[J].江苏农业学报,2017,(02):241.[doi:doi:10.3969/j.issn.1000-4440.2017.02.001]
　FANG Xian-wen,ZHANG Yun-hui,XIAO Xi-lin,et al.Mapping of QTLs for grain shape using recombinant inbred lines in rice (Oryza sativa L.)[J].,2017,(06):241.[doi:doi:10.3969/j.issn.1000-4440.2017.02.001]

备注/Memo

备注/Memo:
收稿日期：2016-06-07 基金项目：国家自然科学基金项目(31571624、31071382)；国家重点基础研究发展计划（2010CB125904、2013CBA01405）；江苏省高校自然科学研究重大项目（15KJA210004）；江苏高校优势学科建设工程项目 作者简介：裔传灯（1973-），男，江苏盐城人，博士，副教授，主要从事水稻遗传育种研究。（Tel）0514-87937619；（E-mail）cdyi@yzu.edu.cn
更新日期/Last Update: 2017-02-07