[1]蒋正宁,别同德,赵仁惠,等.受条锈菌诱导的小麦丝氨酸苏氨酸激酶基因TaS/TK的克隆与表达[J].江苏农业学报,2016,(05):980-986.[doi:10.3969/j.issn.1000-4440.2016.05.004]
 JIANG Zheng-ning,BIE Tong-de,ZHAO Ren-hui,et al.Cloning and expression analysis of a Serine/Threonine protein kinase gene TaS/TK in wheat in response to stripe rust fungal infection[J].,2016,(05):980-986.[doi:10.3969/j.issn.1000-4440.2016.05.004]
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受条锈菌诱导的小麦丝氨酸苏氨酸激酶基因TaS/TK的克隆与表达()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年05期
页码:
980-986
栏目:
遗传育种·生理生化
出版日期:
2016-11-22

文章信息/Info

Title:
Cloning and expression analysis of a Serine/Threonine protein kinase gene TaS/TK in wheat in response to stripe rust fungal infection
作者:
蒋正宁别同德赵仁惠高德荣吴旭江张伯桥
江苏里下河地区农业科学研究所/农业部长江中下游小麦生物与遗传改良重点实验室,江苏扬州225007
Author(s):
JIANG Zheng-ningBIE Tong-deZHAO Ren-huiGAO De-rongWU Xu-jiangZHANG Bo-qiao
Key Laboratory of Wheat Biology and Genetic Improvement on Low & Middle Yangtze River Valley Winter Wheat Region, Ministry of Agriculture/Institute of Agricultural Science of the Lixiahe District in Jiangsu Province, Yangzhou 225007,China
关键词:
小麦条锈病丝氨酸苏氨酸激酶基因实时定量PCR
Keywords:
wheatstripe rustSerine/Threonine kinase genereal time quantitative PCR(qRT-PCR)
分类号:
S512.1
DOI:
10.3969/j.issn.1000-4440.2016.05.004
文献标志码:
A
摘要:
以小麦基因芯片数据获得的1个受条锈病菌诱导上调表达的EST序列为探针,从小麦中克隆抗条锈病相关基因TaS/TK,并对其序列特征、进化关系和表达特征进行分析。结果表明:TaS/TK基因cDNA全长为1 582 bp,包含1 239 bp的ORF、139 bp的5′UTR以及240 bp的3′UTR,编码412个氨基酸,分子量47 120,等电点为5.84,并将其定位于小麦5B染色体;SMART软件分析结果表明TaS/TK仅存在1个典型的丝氨酸/苏氨酸蛋白激酶结构域;经激酶区Blastp比对分析,TaS/TK与其他植物同源性较低,与同源性最高的短柄草仅为59%;qRT-PCR检测结果显示,TaS/TK在小麦茎、叶中均有表达,但在根中低水平表达;TaS/TK在抗病小麦、感病小麦中均受条锈菌诱导表达,但在抗病材料中表达水平明显高于感病材料;同时,TaS/TK受水杨酸诱导上调表达。以上结果表明TaS/TK可能参与小麦SA信号通路中对条锈病菌的抗性反应。
Abstract:
In this study, using an up-regulated EST screened by gene chip as probe, a stripe rust resistance-related gene TaS/TK was cloned from wheat,and the gene sequence features, evolutionary relationships and expression characteristics were analyzed. The full-length cDNA sequence of TaS/TK is 1 582 bp in length, containing a 1 239-bp open reading frame(ORF), a 139-bp 5′ UTR and a 240-bp 3′ UTR. TaS/TK was predicted to encode a protein of 412 amino acids with a molecular mass of 47 120 and an isoeletric point (pI) of 5.84, and was located on chromosome 5B. SMART analysis revealed this putative protein (TaS/TK) only possessed the activities of the serine/threonine kinase. Phylogenetic analysis showed that TaS/TK was lowly homologous to the orthologous genes from rice, maize and barley, and the highest similarity (59%) was to that from Brachypodium distachyon. Real time quantitative PCR exhibited low expression of TaS/TK in roots but high in stems and leaves. TaS/TK was obviously responsive to stripe rust pathogen, and presented rather higher expression in resistant materials than in susceptible materials. Furthmore, the transcription level of TaS/TK was strongly induced by salicylic acid (SA).These results imply that TaS/TK might be involved in the SA-dependent stripe rust resistance responding gene network in wheat.

参考文献/References:

[1]WAN A M, ZHAO Z H, CHEN X M, et al. Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici[J]. Plant Disease,2004,88(8):896-904.
[2]SHARMA A,KOMATSU S. Involvement of Ca2+-dependent protein kinase component downstream to the gibberellin-binding phosphoprotein, Rubis CO activase, in rice[J]. BBRC, 2002, 290(2): 690-695.〖JP〗
[3]WALKER J C,ZHANG R.Relationship of a putative receptor protein kinase from maize to the S-locus glycoprotein of Brassica[J].Nature,1990,345(627): 743-746.
[4]邹晓威,王娜,刘芬,等. 玉米抗病相关基因在玉米与玉米丝黑穗病菌、玉米黑粉病菌互作过程中的表达差异分析[J]. 江苏农业科学, 2014,42(11): 150-152.
[5]FERREIRA P C,HEMERLY A S,VILLARROEL R,et al.The arabidopsis functional homolog of P34cdc2 protein kinase[J].Plant Cell,1991,3 (5):531-540.
[6]LIN X,FENG X H,WATSON J C. Differential accumulation of transcriptsencoding protein kinase homologs in greening pea seedling[J].Proc Natl Acad Sci USA,1991,88(16): 6951-6955.
[7]ANDERBERG R J,WALKER S M K.Isolation of a wheat cDNA clone for an abscisic acid-inducible transcript with homology to pro-tein kinases[J]. Proc Natl Acad Sci USA,1992,89(21): 10183-10187.
[8]张照贵,李冰,王佳佳,等. 小麦 SnRK2.2 基因克隆、表达载体构建及转化[J].山东农业科学,2014,46(11): 1-7.
[9]PAY A,JONAK C,BOGRE L,et al.The Ms K family of alfalfa protein kinase genes encodes homologues of shaggy/glycogen synthase kinase3 and shows differential expression patterns in plant organs and development[J].Plant Jouranl, 1993,3(6):847-856.
[10]MARTIN G B,BROMMONSCHENKEL S H,CHUNWONGSE J,et al.Map-basedcloning of a protein kinase gene conferring disease resistance in tomato[J]. Science,1993,262(5138): 1432-1436.
[11]ITO Y,BANNO H,MORIBE T,et al. NPK15,a tobacco protein-serine/threonine kinase with a single hydrophobic region near the amino terminus[J].Mo1 Gen Genet,1994,245(1): 1-10.
[12]ZHANG C,ZHAO H,LIU Y,et al. Isolation and characterization of a novel glycogen synthase kinase gene, Gm GSKin Glycine max L.that enhances abiotic stress tolerance in Saccbaromyces cerevisiae[J].Biotechnology Letters,2010,32(6): 861-866.
[13]〖JP2〗董海涛,吴玉良,程志强,等.差异显示法克隆水稻抗白叶枯病相关蛋白激酶基因[J]. 浙江大学学报, 1998, 24(5): 548-552.〖JP〗
[14]赵丽,张国嘉,郑世刚,等. 水稻类受体蛋白激酶 OsESG1 的原核表达[J].山东农业科学,2015,47(1): 1-5.
[15]许园园,李晓刚,李慧,等. 梨 CDPK 基因家族全基因组序列鉴定分析[J]. 江苏农业学报,2015,31(3):659-666.
[16]SONG W Y,WANG G L,CHEN L,et al. A receptor kinase-like protein encoded by the rice disease resistance gene,Xa21[J]. Science,1995,270 (15): 1804-1806.
[17]ZHOUJ M,TANG X Y, MARTIN G B. The Pto kinase conferring resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis-related genes [J].The EMBO Journal, 1997, 16(11): 3207-3218.
[18]BRUEGGEMAN R, DRADER T, KLEINHOFS A. The barley ser/thr kinase gene rpg1 providing resistance to stem rust belongs to a gene family with five other members encoding kinase domains[J]. Theor Appl Genet, 2006, 114(6): 1147-1158.
[19]〖JP2〗FEUILLET C, SCHACHERMAYR G, KELLER B. Molecular cloning of a new receptor-like kinase gene encoded at the Lr10 disease resistance locus in wheat[J]. Plant J, 1997, 11(1): 45-52.〖JP〗
[20]CAO A Z, XING L P, WANG X Y, et al.Serine/threonine kinase gene Stpk-V, a key member ofpowdery mildew resistance gene Pm21, conferspowdery mildew resistance in wheat[J].PNAS, 2011,108(19):7727-7732.
[21]康振生, 王瑶, 黄丽丽 等.小品种对条锈病低反应型麦抗性的组织学和超微结构研究[J].中国农业科学,2003,36(9):1026-1031.
[22]〖JP3〗RUDRABHATLA P,REDDY M M,RAJASEKHARAN R.Genome-wide analysis and experimentation of plant serine/threonine/tyrosine-speci protein kinases[J].Plant Mol Biol,2006,60(2):293-319.〖JP〗
[23]GE R C,CHEN G P,SHEN Y Z,et al.Cloning and functional characterization of a wheat serine/threonine kinase gene (TaSTK) related to salt-resistance[J]. Plant Sicence, 2007,173(1):55-60.
[24]DONG X. SA, JA, ethylene, and disease resistance in plants[J]. Current Opinion in Plant Biology, 1998, 1(4):316-323.
[25]MA J X, ZHOU R H, DONG Y S, et al.Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers[J]. Euphytica, 2001, 120(2):219-226.

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备注/Memo

备注/Memo:
收稿日期:2016-03-01 基金项目:国家重点研发计划项目(2016YFD0101802);江苏省自然科学青年基金项目(BK20140500);江苏省农业科技自主创新基金项目[CX(14)5080] 作者简介:蒋正宁(1979-),男,江西乐平人,博士,助理研究员,研究方向为小麦分子遗传学。(Tel)13665219751;(E-mail)znjiang79@aliyun.com 通讯作者:张伯桥,(E-mail)zbq@wheat.org.cn.
更新日期/Last Update: 2016-11-22