[1]刘凯,王爱民,严国红,等.一个水稻显性矮秆突变体的遗传特性与降株高能力[J].江苏农业学报,2016,(05):968-973.[doi:10.3969/j.issn.1000-4440.2016.05.002]
　LIU Kai,WANG Ai-min,YAN Guo-hong,et al.Genetic analysis and plant height reduction of a dominant dwarf mutant of rice[J].,2016,(05):968-973.[doi:10.3969/j.issn.1000-4440.2016.05.002]
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一个水稻显性矮秆突变体的遗传特性与降株高能力()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年05期
页码:
968-973
栏目:
遗传育种·生理生化
出版日期:
2016-11-22

文章信息/Info

Title:
Genetic analysis and plant height reduction of a dominant dwarf mutant of rice
作者:
刘凯; 王爱民; 严国红; 唐红生; 孙明法
江苏沿海地区农业科学研究所，江苏盐城224002
Author(s):
LIU Kai; WANG Ai-min; YAN Guo-hong; TANG Hong-sheng; SUN Ming-fa
Institute of Agricultural Sciences in the Coastal District of Jiangsu Province, Yancheng224002,China
关键词:
水稻; 矮秆; 突变体; 降秆能力
Keywords:
rice; dwarf; mutant; plant height reduction
分类号:
S511.024
DOI:
10.3969/j.issn.1000-4440.2016.05.002
文献标志码:
A
摘要:
通过EMS（甲基磺酸乙酯）诱变获得遗传稳定的水稻显性矮秆突变体Dy。与野生型盐恢269相比，突变体表现株高明显矮化，主要农艺性状（穗长、粒长、粒宽、分蘖数、千粒质量、结实率、抽穗期）均发生显著改变。赤霉素（GA3）和油菜素类固醇（BR）敏感性分析结果表明，Dy中GA3含量显著高于野生型，经GA3处理后，Dy的第2茎节长与野生型无显著差异，表明Dy对GA3不敏感；而经BR处理后，Dy的第2茎节伸长受到明显抑制，表明Dy对BR敏感。遗传分析结果表明，突变体Dy受1对显性核基因控制。以突变体Dy/Dular的F2群体作为基因定位群体，将该基因定位于第3条染色体长臂SSR标记YC327和YC329之间，遗传距离为0.93 cM。通过与7个籼稻恢复系和8个常规粳稻品种杂交F1株高的调查，发现突变体Dy具有较强的降株高能力，降株高率最高达41.23%。
Abstract:
A dominant dwarfmutant Dy of rice with stable inheritance was mutagenized by ethylmethylsulfone mutation. Compared with wild-type Yanhui 269, the Dy plant was significantly lower, and main agronomic traits (panicle length, grain length, grain width, number of panicles per plant, 1 000-grain weight, seed setting rate, heading date) changed. The gibberellin (GA3) content in Dy was significantly higher than that in wild type. The second stem node length of GA3-treated Dy showed in significant difference with that of GA3-treated wild type, indicative of the insensitivity of Dy. The elongation of second stem node of Dy was inhibited by brassinosteroid (BR) application, suggesting that Dy is a BR defective mutant. Genetic analysis revealed that Dy was controlled by one pair of dominant nuclear genes. With Dy/Dular F2 population as gene mapping population,the mutative gene was initially mapped within the long arm of chromosome 3 between SSR markers YC327 and YC329，with the genetic distance of 0.93 cM.The height survey of F1 hybrids of seven indica restorer and eight japonica suggests that Dy is strong in reducing plant height, with the reduction rate of 41.23%.

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备注/Memo

备注/Memo:
收稿日期：2016-01-19 基金项目：江苏省自然科学基金项目(BK20161308); 江苏省农业科技自主创新基金项目[CX（12）5072]；国家科技支撑计划项目（2015BAD01B03）。 作者简介：刘凯（1984-），男，河南南阳人，硕士，助理研究员，从事水稻分子育种工作。（E-mail）liukai11121@163.com 通讯作者：孙明法，（E-mail）smf559@163.com
更新日期/Last Update: 2016-11-22