[1]刘景坤,武春媛,贾明明,等.液质联用法测定辣椒中噻虫嗪残留[J].江苏农业学报,2016,(04):934-938.[doi:10.3969/j.issn.100-4440.2016.04.035]
 LIU Jing-kun,WU Chun-yuan,JIA Ming-ming,et al.A LC-MS method for detection of thiamethoxam residue in pepper[J].,2016,(04):934-938.[doi:10.3969/j.issn.100-4440.2016.04.035]
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液质联用法测定辣椒中噻虫嗪残留()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年04期
页码:
934-938
栏目:
加工贮藏·质量安全
出版日期:
2016-10-31

文章信息/Info

Title:
A LC-MS method for detection of thiamethoxam residue in pepper
作者:
刘景坤12武春媛12贾明明13邓晓12吴东明14李怡12李勤奋12
1.中国热带农业科学院环境与植物保护研究所,海南 海口 571101;2.农业部儋州农业观测站,海南 儋州 571737;3.华中农业大学,湖北 武汉 430070;4.海南大学,海南 海口 570228
Author(s):
LIU Jing-kun12WU Chun-yuan12JIA Ming-ming13DENG Xiao12WU Dong-ming14LI Yi12LI Qin-fen 12
1.Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;2. Danzhou Scientific Observing and experimental Station of Agro-environment, Ministry of Agriculture, Danzhou 571737, China;3. Huazhong Agricultural University, Wuhan 430070,China;4. Hainan University,Haikou 570228, China
关键词:
噻虫嗪液质联用基质效应回收率
Keywords:
thiamethoxam LC-MS matrix effect recovery
分类号:
X836
DOI:
10.3969/j.issn.100-4440.2016.04.035
文献标志码:
A
摘要:
为测定辣椒中的噻虫嗪残留,选取乙腈为提取剂,采用匀浆、超声波两种方法进行提取,采用SPE(NH2)柱、PSA管两种净化方法进行样品净化,分别比较不同提取及净化方法的基质效应及准确度。结果表明,两种提取方法的基质效应都较小,且无明显差异,超声波法虽然方便、高效,但加标回收率较低,准确性较差无法满足要求;虽然SPE柱净化较PSA管净化基质效应稍小,但二者没有明显差异,且PSA管净化回收率略高,因此选取操作简便、高效率的PSA净化方法。标准曲线在0.005~0.500 μg/ml线性关系良好, R2=0.999 7,该方法检出限为0.008 μg/g, 能够准确进行实际样品检测。
Abstract:
The LC-MS/MS equipment was used for the detection of thiamethoxam residue in the peppers. The matrix effect (ME) was studied, and the extraction methods of homogenization and ultrasonic, the purifications with SPE columns and PSA tubes were compared using acetonitrile as extraction solution. No matrix effect was detected. In spite of simple operation and high efficiency, ultrasonic extraction is outperformed by homogenized extraction in spiked recovery. PSA tubes were selected in purification over SPE column due to faster operation and less organic solvent. By applying homogenized extraction and PSA tube purification in the pre-treatment, LC-MS accurately detected the thiamethoxam in pepper with detect limit of 0.008 μg/g.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-11-16基金项目:中央级公益性科研院所基本科研业务费项目(2015hzs1J026);公益性行业(农业)科研专项经费项目(201503107)作者简介:刘景坤(1986-),男,山东临沭人,硕士,助理研究员,研究方向为农产品质量安全与农业环境保护。(Tel)0898-66969272;(E-mail)liujk918@163.com通讯作者:李勤奋,(E-mail)qinfenli2005@163.com
更新日期/Last Update: 2016-11-01