[1]王士磊,丁正权,黄海祥.水稻隐性早熟突变体ref早熟性的遗传分析和基因定位[J].江苏农业学报,2016,(04):721-724.[doi:10.3969/j.issn.100-4440.2016.04.001]
　WANG Shi-lei,DING Zheng-quan,HUANG Hai-xiang.Inheritance and gene mapping of recessive earliness in rice (Oryza sativa L.)[J].,2016,(04):721-724.[doi:10.3969/j.issn.100-4440.2016.04.001]
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水稻隐性早熟突变体ref早熟性的遗传分析和基因定位()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年04期
页码:
721-724
栏目:
遗传育种·生理生化
出版日期:
2016-10-31

文章信息/Info

Title:
Inheritance and gene mapping of recessive earliness in rice (Oryza sativa L.)
作者:
王士磊; 丁正权; 黄海祥
嘉兴市农业科学研究院，浙江 嘉兴 314016
Author(s):
WANG Shi-lei; DING Zheng-quan; HUANG Hai-xiang
Jiaxing Academy of Agricultural Sciences, Jiaxing 314016, China
关键词:
水稻; 隐性早熟突变体; 抽穗期; 遗传分析; 基因定位
Keywords:
rice (Oryza sativa L.); recessive earliness; heading date; inheritance; gene mapping
分类号:
S511.032
DOI:
10.3969/j.issn.100-4440.2016.04.001
文献标志码:
A
摘要:
为了更好地理解水稻早熟性的遗传机制，对隐性早熟突变体ref进行了遗传分析和基因定位。突变体ref对光周期不敏感，与6个晚熟品种（系）杂交F1代均为晚熟。ref×嘉禾218 F2分离群体抽穗期呈现连续双峰分布，早抽穗和晚抽穗之比符合1∶3的分离比，表明ref早熟性主要受1对隐性早熟基因控制。F2群体中1 005株早熟和晚熟单株组成定位群体，混合基因池分析发现5号染色体上SSR标记RM7302和RM3853与突变体ref早熟基因连锁。构建5号染色体的连锁图谱，进行表型和标记基因型的连锁分析，进一步确认突变体ref早熟基因定位在标记RM7302和RM3853之间18.32 cM的遗传区间内。
Abstract:
To uncover the genetic mechanism regulating early flowering in rice, a newly identified mutant ref was genetically analyzed and mapped for earliness genes. Ref was insensitive to photoperiodic response. The F1 progenies, derived from ref and six late flowering accessions, expressed late flowering. The heading date in ref×Jiahe218 F2 population displayed continuous and bimodal frequency distributions, and the ratio early flowering plants to late flowering plants followed a 1∶3 segregation ratio, suggesting that earliness was controlled by a pair of recessive genes. A total of 1 005 early/late flowering plants derived from F2 generation was selected to construct the gene mapping population. Bulked segregation analysis identified SSR markers RM7302 and RM3853 on chromosome 5 associated with earliness genes. With further linkage analysis, earliness gene was finally mapped to an interval of 18.32-cM region between SSR markers RM7302 and RM3853 on the genetic map of chromosome 5.

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备注/Memo

备注/Memo:
收稿日期：2015-12-27基金项目：嘉兴市科技项目（2010AZ2017、2011AZ1021、2012AY1053、2013BZ26001）；浙江省公益项目（2013C32028）作者简介：王士磊（1983-），男，山东莘县人，硕士，农艺师，从事水稻遗传育种工作。（Tel）15888309034；(E-mail)wangshilei2010@126.com通讯作者：黄海祥,（Tel）0573-83777114；(E-mail)jiahe218@live.cn
更新日期/Last Update: 2016-11-01