[1]刘洁霞,谭国飞,吴雪君,等.鸭儿芹异黄酮2’-羟化酶基因的克隆及其表达的温度响应[J].江苏农业学报,2016,(03):640.[doi:10.3969/j.issn.1000-4440.2016.03.024]
　LIU Jie-xia,TAN Guo-fei,WU Xue-jun,et al.Cloning of isoflavone 2’-hydroxylase gene from Cryptotaenia japonica Hassk and its expression in response to temperature[J].,2016,(03):640.[doi:10.3969/j.issn.1000-4440.2016.03.024]
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鸭儿芹异黄酮2’-羟化酶基因的克隆及其表达的温度响应()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年03期
页码:
640
栏目:
园 艺
出版日期:
2016-06-30

文章信息/Info

Title:
Cloning of isoflavone 2’-hydroxylase gene from Cryptotaenia japonica Hassk and its expression in response to temperature
作者:
刘洁霞;  谭国飞;  吴雪君;  许瀛之;  陈佳凯;  熊爱生
南京农业大学园艺学院作物遗传与种质创新国家重点实验室,江苏 南京 210095
Author(s):
LIU Jie-xia;  TAN Guo-fei;  WU Xue-jun;  XU Ying-zhi;  CHEN Jia-kai;  XIONG Ai-sheng
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
关键词:
鸭儿芹;  CjI2’H基因;  克隆;  实时荧光定量PCR;  温度胁迫;  基因表达
Keywords:
Cryptotaenia japonica Hassk;  CjI2’H gene;  cloning;  quantitative real-time PCR;  temperature stress;  gene expression
分类号:
S636.3
DOI:
10.3969/j.issn.1000-4440.2016.03.024
文献标志码:
A
摘要:
以贵州野生鸭儿芹为材料,研究了与植物抗逆境胁迫有关的细胞色素P450(CYP450)家族成员异黄酮2’-羟化酶(I2’H)基因对温度胁迫的应答和响应。利用PCR扩增技术克隆得到鸭儿芹异黄酮2’-羟化酶基因CjI2’H,该基因的开放阅读框长为1 527 bp,可编码508个氨基酸,通过DNAMAN软件预测其蛋白质分子质量为 1.270 9×105,等电点(pI)为4.80。进化树分析结果表明CjI2’H编码的氨基酸序列在不同植物间相对保守。实时定量PCR结果显示鸭儿芹在30 ℃处理下,叶柄中CjI2’H基因的相对表达量随处理时间的增加而上升,且在24 h 时达到峰值; 10 ℃处理下,CjI2’H基因的相对表达量明显低于高温处理。说明CjI2’H基因表达与温度胁迫存在关联性,高温胁迫后该基因的表达响应大于低温胁迫。
Abstract:
The CjI2’H gene encoding isoflavone 2’-hydroxylase(I2’H), a member of the cytochrome P450 superfamily related to abiotic stress in plants, was cloned by PCR amplification from wild Cryptotaenia japonica Hassk of Guizhou province. It’s ORF(open reading frame)length is 1 527 bp, encoding 508 amino acids. The enzyme molecular weight predicted by DNAMAN software was 1.270 9×105, and the pI was 4.80. The phylogenetic analysis showed that the CjI2’H-encoded amino acid sequences of I2’H were conservative in different plant species. The expression level of CjI2’H gene in the petiole of C. japonica Hassk at 30 ℃ detected by quantitative real-time PCR were up-regulated and peaked at 24 h. At 10 ℃, the expression level of CjI2’H was lower than that at high temperature. The expression of CjI2’H gene is related to temperature in C. japonica Hassk, greater under high temperature stress.

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备注/Memo

备注/Memo:
收稿日期:2015-09-21
基金项目:国家自然科学基金项目(31272175); 江苏省杰出青年基金项目(BK20130027); 江苏高校优势学科建设项目
作者简介::刘洁霞(1994-),女, 山西晋中人,本科生,主要从事蔬菜分子生物学研究。(E-mail)14112307@njau.edu.cn
通讯作者:熊爱生,(E-mail)xiongaisheng@njau.edu.cn
更新日期/Last Update: 2016-06-30