[1]曹东阳,王小敏,钱爱东,等.江苏省及周边地区猪圆环病毒Ⅱ型(PCV2)分子流行病学调查[J].江苏农业学报,2016,(02):390-398.[doi:10.3969/j.issn.1000-4440.2016.02.024]
　CAO Dong-yang,WANG Xiao-min,QIAN Ai-dong,et al.Molecular epidemiological investigation of porcine circovirus type 2(PCV2)in Jiangsu and surrounding areas[J].,2016,(02):390-398.[doi:10.3969/j.issn.1000-4440.2016.02.024]
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江苏省及周边地区猪圆环病毒Ⅱ型(PCV2)分子流行病学调查()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年02期
页码:
390-398
栏目:
畜牧兽医·水产养殖
出版日期:
2016-03-20

文章信息/Info

Title:
Molecular epidemiological investigation of porcine circovirus type 2(PCV2)in Jiangsu and surrounding areas
作者:
曹东阳1; 2;  王小敏2;  钱爱东1;  何孔旺2;  茅爱华2
1.吉林农业大学动物科学技术学院,吉林 长春 130118; 2.江苏省农业科学院兽医研究所/农业部兽用生物制品工程技术重点实验室/国家兽用生物制品工程技术研究中心,江苏 南京 210014
Author(s):
CAO Dong-yang1; 2;  WANG Xiao-min2;  QIAN Ai-dong1;  HE Kong-wang2;  MAO Ai-hua2
1.College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; 2.Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/ Key Laboratory of Engineering Research of Veterinary Bio-products, Ministry of Agriculture/ National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China
关键词:
猪圆环病毒Ⅱ型;  分子流行病学;  遗传变异
Keywords:
porcine circovirus type 2(PCV2);  molecular epidemiology;  genetic variation
分类号:
S852.65+1
DOI:
10.3969/j.issn.1000-4440.2016.02.024
文献标志码:
A
摘要:
为了解中国江苏省及周边地区猪圆环病毒Ⅱ型(PCV2)的分子流行病学以及毒株的遗传变异情况,本研究运用PCR技术对来源于不同地区、不同猪场且PCV2检测为阳性的样品进行全基因组序列的扩增和测序,共获得18株PCV2全基因序列,并对所得到的毒株进行遗传变异分析。结果表明,18株毒株与国内外的参考毒株同源性为 93.5%～99.7%,而ORF2核苷酸及其推导的氨基酸序列同源性分别为 87.1%～99.9%和 84.2%～99.6%,说明毒株存在一定程度的变异。系统进化树分析结果显示18株毒株中有5株PCV2a,7株PCV2b,6株PCV2d,其中20150429YC全长为1 766 bp,与(HM038031.1 PCV2d等)参考毒株的同源性为100.0%,未发现PCV2c。同时,Cap蛋白的抗原表位和抗原性也发生了变化。说明,目前江苏省及周边地区猪群中PCV2感染较为普遍,PCV2的流行毒株以PCV2b基因型为主,PCV2d次之。
Abstract:
Porcine circovirus type 2(PCV2)positive samples from Jiangsu province and surrounding areas were examined by PCR and sequenced to get the complete viral genome. Total 18 isolates were identified and analyzed for their genetic variations. For viral genomes, 18 isolates shared 93.5%-99.7% similarities with reference viruses. For ORF2 nucleotide and amino acid sequences, 87.1%-99.9% and 84.2%-99.6% similarities were found between the examined isolates and references, respectively, indicating certain level of genetic variations among PCV2 isolates. Among 18 isolates, there were 5 PCV2a strains, 7 PCV2b strains, and 6 PCV2d strains, and no PCV2c was found. One tested isolate, 20140429YC with full length of 1 766 bp, was identical to the PCV2d reference sequence(HM038031.1).There were changes in the epitopes and antigenicity of ORF2 encoded protein. This study indicated that the epidemic situation of PCV2 in Jiangsu province and surrounding areas was fairly common, and PCV2b was the dominant subtype and PCV2d was the second.

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备注/Memo

备注/Memo:
收稿日期:2015-09-02 基金项目:国家自然科学基金项目(31302071); 公益性行业(农业)科研专项(201303046); 江苏省农业科技自主创新基金项目[CX(14)2045]; 江苏省第四期“333高层次人才培养工程”(第二层次)资助项目(BRA2012194) 作者简介:曹东阳(1989-),女,吉林长春人,硕士研究生,研究方向:微生物与免疫。(E-mail)569212988@qq.com 通讯作者:钱爱东,(E-mail)qianaidong0115@163.com
更新日期/Last Update: 2016-03-20