[1]庞有志,白俊艳,张小辉,等.北京白羽鹌鹑微卫星多态性分析[J].江苏农业学报,2015,(05):1110-1114.[doi:doi:10.3969/j.issn.1000-4440.2015.05.026]
　PANG You-zhi,BAI Jun-yan,ZHANG Xiao-hui,et al.Polymorphism analysis of microsatellite marker of Beijing white-feather quail[J].,2015,(05):1110-1114.[doi:doi:10.3969/j.issn.1000-4440.2015.05.026]
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北京白羽鹌鹑微卫星多态性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
1110-1114
栏目:
畜牧兽医·水产养殖
出版日期:
2015-10-31

文章信息/Info

Title:
Polymorphism analysis of microsatellite marker of Beijing white-feather quail
作者:
庞有志; 白俊艳; 张小辉; 赵淑娟; 吴胜军; 于美琴; 许华伟
（河南科技大学动物科技学院,河南洛阳471003）
Author(s):
PANG You-zhi; BAI Jun-yan; ZHANG Xiao-hui; ZHAO Shu-juan; WU Sheng-jun; YU Mei-qin; XU Hua-wei
（College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China）
关键词:
北京白羽鹌鹑; 微卫星; 遗传多样性
Keywords:
Beijing white-feather quail; microsatellite marker; genetic diversity
分类号:
S839
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.026
文献标志码:
A
摘要:
为了从分子水平上揭示北京白羽鹌鹑群体的遗传结构，为北京白羽鹌鹑遗传资源的评价、保护和利用提供新的依据，采用PCR扩增、聚丙烯酰胺凝胶电泳等方法对北京白羽鹌鹑群体9个微卫星标记(GUJ0023、GUJ0028、GUJ0029、GUJ0057、GUJ0059、GUJ0063、GUJ0077、GUJ0083、GUJ0097)的遗传多样性进行分析。结果表明，9个微卫星标记共检测出43个等位基因，平均每个标记检测到4.777 8个等位基因。平均杂合度为0.704 6，平均多态信息含量（PIC）为0.658 7，平均有效等位基因数为3.613 5。除GUJ0063（PIC=0.463 9）在北京白羽鹌鹑群体中为中度多态性标记外，其余8个微卫星基因座均为高度多态性标记，可以作为北京白羽鹌鹑群体遗传多样性分析的有效遗传标记。微卫星基因座GUJ0023和GUJ0029在白羽鹌鹑群体中的χ2值小于χ20.01，符合哈迪-温伯格定律，其余微卫星基因座均偏离哈迪-温伯格平衡定律，这种遗传不平衡可能与近年来人们对北京白羽鹌鹑进行小群体保种有关。
Abstract:
To reveal the genetic structure of Beijing white-feather quail at molecular level and to provide new evidence for the evaluation, protection and utilization of genetic resources of Beijing white-feather quail, the genetic diversity of Beijing white-feather quail population was analyzed with 9 microsatellite markers using PCR amplification, polyacrylamide gel electrophoresis. Totally 43 alleles were detected in 9 microsatellite markers GUJ0023， GUJ0028， GUJ0029， GUJ0057， GUJ0059， GUJ0063， GUJ0077， GUJ0083， GUJ0097, 4.777 8 for each marker on average. The average heterozygosity is 0.704 6, the average polymorphism information content (PIC) is 0.658 7 and the average effective allele is 3.613 5. Except for marker GUJ0063 (PIC=0.463 9) which was moderately polymorphic, the other 8 microsatellite markers showed high polymorphism which could serve as the effective genetic markers to analyze the genetic diversity of Beijing white-feather quail. Only GUJ0023 and GUJ0029 achieved the genetic distribution satisfying Hardy-Weinberg law in the white-feather quail population, while the rest markers significantly deviated from the Hardy-Weinberg balance (P<0.01). The genetic disequilibrium might be associated with the small group conservation of Beijing white-feather quail in recent years.

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备注/Memo

备注/Memo:
收稿日期：2015-02-27 基金项目：河南省重点科技攻关项目 (082102130002) 作者简介：庞有志（1963-），男，河南新蔡人，博士，教授，主要从事动物遗传育种研究。（E-mail）pyzh2006@126.com
更新日期/Last Update: 2015-10-31