[1]朱淑斌,周春宝,韩大勇,等.姜曲海猪FoxO1基因遗传多态性及与肉质性状的相关分析[J].江苏农业学报,2015,(05):1065-1069.[doi:doi:10.3969/j.issn.1000-4440.2015.05.019]
 ZHU Shu-bin,ZHOU Chun-bao,HAN Da-yong,et al.Genetic polymorphism of FoxO1 gene and correlation with meat quality of Jiangquhai swine[J].,2015,(05):1065-1069.[doi:doi:10.3969/j.issn.1000-4440.2015.05.019]
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姜曲海猪FoxO1基因遗传多态性及与肉质性状的相关分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
1065-1069
栏目:
畜牧兽医·水产养殖
出版日期:
2015-10-31

文章信息/Info

Title:
Genetic polymorphism of FoxO1 gene and correlation with meat quality of Jiangquhai swine
作者:
朱淑斌1周春宝1韩大勇1倪黎纲12陶勇1赵旭庭1王平安1
(1.江苏农牧科技职业学院,江苏泰州225300;2.姜曲海猪保种场,江苏泰州225300)
Author(s):
ZHU Shu-bin1ZHOU Chun-bao1HAN Da-yong1NI Li-gang12TAO Yong1ZHAO Xu-ting1WANG Ping-an1
(1.Jiangsu Animal Husbandry and Veterinary College, Taizhou 225300, China;2.Jiangquhai Pig Breed Conservation Farm, Taizhou 225300, China)
关键词:
姜曲海猪FoxO1基因PCR-RFLP肉质性状
Keywords:
Jiangquhai swineFoxO1 genePCR-RFLPmeat quality
分类号:
S828.2
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.019
文献标志码:
A
摘要:
为探究姜曲海猪FoxO转录因子家族中FoxO1基因的多态性及其与肉质性状的相关性,采用内切酶Bpil PCR-RFLP分子标记技术,分析了112头姜曲海猪FoxO1基因外显子1的遗传变异情况。结果表明,姜曲海猪试验猪群Bpil酶切位点存在多态性,酶切得到EE、Ee、ee 3种基因型,E基因频率为0.834 8,表现为低度多态(PIC=0.237 8);试验猪群的基因频率和基因型频率都处于哈迪温伯格平衡状态(P>0.05)。分析不同基因型对肉质性状的影响, 发现FoxO1基因第1外显子Bpil酶切位点多态性与猪肌内脂肪含量、大理石纹和剪切力性状关联显著。可见,姜曲海猪FoxO1基因的多态性对其肉质性状有重要的影响。 
Abstract:
To identify the polymorphism of transcription factor family member FoxO1 gene and its correlation with meat quality of swine, PCR-RFLP (Bpil restriction enzyme) was employed to study the genetic variation of FoxO1 gene exon 1 in 112 Jiangquhai swine. Three polymorphic loci were detected in FoxO1 exon 1 with three genotypes (EE/Ee/ee). The frequency of allele E was 0.834 8, showing low polymorphism (PIC=0.237 8). The gene frequencies and genotype frequencies of swine followed Hardy-Weinberg law (P>0.05). The polymorphism in FoxO1 in exon 1 showed significant correlations wih intramuscular fat (IMF) content, marbling and shear stress. It was obvious that the polymophism of FoxO1 gene might contribute to meat quality.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-05-21 基金项目:发改委生物育种能力与产业化专项[(2014)2573];江苏农牧科技职业学院重点课题(NSFZD1301);大学生创新创业训练计划项目 作者简介:朱淑斌(1977-),女,黑龙江哈尔滨人,博士,副教授,研究方向为动物遗传育种。(E-mail)zhushubin2003@163.com
更新日期/Last Update: 2015-10-31