[1]吴祥,姚克兵,吉沐祥,等.句容地区草莓枯萎病病原菌的分离鉴定及田间防治[J].江苏农业学报,2015,(04):764-770.[doi:10.3969/j.issn.1000-4440.2015.04.009]
 WU Xiang,YAO Ke-bing,JI Mu-xiang,et al.Isolation and identification and field control of strawberry fusarium wilt in Jurong, Jiangsu province[J].,2015,(04):764-770.[doi:10.3969/j.issn.1000-4440.2015.04.009]
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句容地区草莓枯萎病病原菌的分离鉴定及田间防治()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年04期
页码:
764-770
栏目:
植物保护
出版日期:
2015-08-31

文章信息/Info

Title:
Isolation and identification and field control of strawberry fusarium wilt in Jurong, Jiangsu province
作者:
吴祥12姚克兵1吉沐祥1陈宏州1杨敬辉1李金凤1王莉莉12
(1.江苏丘陵地区镇江农业科学研究所,江苏句容212400;2.江苏省绿盾植保农药实验有限公司,江苏句容212444)
Author(s):
WU Xiang12YAO Ke-bing1JI Mu-xiang1CHEN Hong-zhou1YANG Jing-hui1LI Jin-feng1WANG Li-li12
(1.Zhenjiang Agricultural Scientific Institute of Jiangsu Hilly Area , Jurong 212400,China;2.Jiangsu Lüdun Plant Protection Pesticide Experimentation Co.〖KG-*4〗, Ltd.〖KG-*4〗, Jurong, 212444 , China)
关键词:
草莓枯萎病病原菌分离鉴定田间防治
Keywords:
strawberryfusarium wilt pathogenfield investigationisolation and identificationfield control
分类号:
S668.4
DOI:
10.3969/j.issn.1000-4440.2015.04.009
文献标志码:
A
摘要:
为了明确句容地区草莓枯萎病病原菌的种类和寻找草莓枯萎病的防治方法,2013年10-11月,分别对句容地区草莓枯萎病病菌进行了田间调查、分离培养、形态学观察描述、DNA序列分析、致病性试验和药剂田间防治试验。结果表明,分离到381块真菌菌落,镰刀菌属的真菌(Fusarium)占72.4%。通过形态特征、DNA测序和致病性试验确认草莓枯萎病病原菌为尖孢镰刀菌(Fusarium oxysoporum Schl.)。田间防治效果以250 g/L吡唑醚菌酯EC 2 000倍液最好,灌根后30 d的防治效果为100%,灌根后51 d的防治效果为93.11%;其次是1%申嗪霉素SC 900倍液,灌根后30 d的防治效果为100%,灌根后51 d的防治效果为58.63%,其他参试药剂 1×109〖KG*3〗CFU/g多黏芽孢杆菌(JX-13)WP 1 000倍液、1 g含1×106个孢子的寡雄腐霉WP 7 000倍液、1 g含6×108有效活性菌数的哈茨木霉T-22GR 500倍液等均有一定的防治效果。
Abstract:
A total of 381 fungal colonies were isolated from the strawberry fields infected with fusarium wilt in Jurong area, Jiangsu province, and cultured to identify the pathogen. Fusarium colonies accounted for 72.4 percent. Morphological characteristics, DNA sequencing, and pathogenicity test revealed that the pathogen was Fusarium oxysoporum Schl. The field control effect was the strongest by 250 g/L pyraclostrobin EC 2 000-time solution, with the efficacies of 100.00% after 30-day root application and 93.11% even after 51-day root application, followed by phenazine-1-carboxylic acid SC 900-time solution with the concentration of 1%. Other fungicides such as paenibaccilus polymyxa, pythium oligandrum, and trichoderma harzianum showed certain control effects on the disease as well.

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备注/Memo

备注/Memo:
收稿日期:2014-12-03 基金项目:江苏省农业科技支撑项目(BE2012378);江苏省 "六大人才高峰"项目(2013-NY-001);镇江市农业科技支撑项目(NY2014029) 作者简介:吴祥(1983-),男,江苏泗洪人,硕士,助理研究员,主要从事农药研究与开发。(Tel)13775391005;(E-mail)wuxianghl@126.com 通讯作者:吉沐祥,(Tel)0511-87274221;(E-mail)jilvdun2800@163.com
更新日期/Last Update: 2015-08-31