[1]王琳,李敏,魏启舜,等.双孢蘑菇培养料发酵过程中细菌群落结构分析[J].江苏农业学报,2015,(03):653-658.[doi:10.3969/j.issn.1000-4440.2015.03.030]
　WANG Lin,LI Min,WEI Qi-shun,et al.Bacterial communities in Agaricus bisporus compost analysed by denaturing gradient gel electrophoresis[J].,2015,(03):653-658.[doi:10.3969/j.issn.1000-4440.2015.03.030]
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双孢蘑菇培养料发酵过程中细菌群落结构分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年03期
页码:
653-658
栏目:
园艺
出版日期:
2015-06-30

文章信息/Info

Title:
Bacterial communities in Agaricus bisporus compost analysed by denaturing gradient gel electrophoresis
作者:
王琳1; 李敏2; 魏启舜1; 张洪海3; 周影1; 赵荷娟1
（1.江苏丘陵地区南京农业科学研究所，江苏南京210046;2.南京农业大学资源与环境学院，江苏南京210095；3.金坛市农林局）
Author(s):
WANG Lin1; LI Min2; WEI Qi-shun1; ZHANG Hong-hai3; ZHOU Ying1; ZHAO He-juan1
(1.Nanjing Institute of Agricultural Science in Jiangsu Hilly Area, Nanjing 210046 ,China;2.College of Natural Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095,China;3.Jintan Bureau of Agriculture and Forestry)
关键词:
双孢蘑菇培养料; 细菌群落结构; 变性梯度凝胶电泳PCR- DGGE
Keywords:
Agaricus bisporus compost; microbial community; denaturing gradient gel electrophoresis
分类号:
S646.1+1
DOI:
10.3969/j.issn.1000-4440.2015.03.030
文献标志码:
A
摘要:
为探明双孢蘑菇培养料发酵过程中细菌群落多样性和组成的演变，获得相关优势菌落的信息，本研究利用变性梯度凝胶电泳 (Denaturing gradient gel electrophoresis，DGGE)对不同季节不同发酵阶段样品的的细菌进行特异性扩增，并选取主要DNA条带进行克隆、测序和生物信息学分析。结果显示，不同发酵时期带谱差异明显。发酵过程中，培养料中主要有芽孢杆菌属、黄杆菌属、杆菌属、假单胞菌属、Solibacillus、嗜热裂孢菌属、高温双歧菌属和未知分类地位的不可培养细菌。不同季节（春季、冬季）的培养料一次发酵过程中细菌多样性变化趋势相似，且发酵结束时细菌菌落结构相似。双孢蘑菇培养料在发酵过程中细菌群落结构至少经历了3个阶段的演替，即建堆初期、一次发酵阶段和二次发酵阶段。双孢蘑菇培养料发酵过程中细菌种群丰富，并随着发酵的不同阶段发生演替，种群多样性受环境温度的影响。双孢蘑菇培养料发酵过程中一直存在的细菌群落与具有木质纤维素降解特性细菌的序列相似度较高，有利于培养料转化为双孢蘑菇栽培基质。
Abstract:
PCR-denaturing gradient gel electrophoresis (DGGE) was emloyed to analyze the V3 regions of bacterial 16S ribosomal DNA collected from the compost for Agaricus bisporus cultivation in spring and winter. The dominant DNA were cloned, sequenced and analyzed. Bacillus sp, Flavobacterium sp., Lysinibacillus sp., Thermus thermophilus，Pseudomonas fragi, Solibacillus silvestris，Thermobifida fusca, and two kinds of uncultured bacteria were identified in the compost. The bacteria in spring and winter showed similar changing trend during phase I composting and similar community at the end of composting. The bacterial community structure differed at initial composting, phase I composting and phase II composting. The community diversity was environmental temperature-dependant. The bacteria existing in the compost all through the process of composting shared high sequence similarity with that of the bacteria degrading lignocellulose, which was favorable for the compost to be applied as culture matrix.

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备注/Memo

备注/Memo:
收稿日期：2015-01-13 基金项目：江苏省农业科技自主创新基金项目[CX(13)5072] 作者简介：王琳（1980-） 女，江苏泰州人，博士，助理研究员，主要从事农业废弃物综合利用和食用菌栽培研究。（Tel）025-86197778；（Email）wanglin0421nj@163.com 通讯作者：赵荷娟,（Email）1154622504@qq.com
更新日期/Last Update: 2015-06-30