[1]张维娜,夏飞,谢骏,等.凝结芽孢杆菌JSSW-07对异育银鲫生长及免疫功能的影响[J].江苏农业学报,2015,(03):630-637.[doi:10.3969/j.issn.1000-4440.2015.03.027]
　ZHANG Wei-na,XIA Fei,XIE Jun,et al.Growth and immunity of Carassius gibelio in response to Bacillus coagulans JSSW-07[J].,2015,(03):630-637.[doi:10.3969/j.issn.1000-4440.2015.03.027]
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凝结芽孢杆菌JSSW-07对异育银鲫生长及免疫功能的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年03期
页码:
630-637
栏目:
畜牧兽医·水产养殖
出版日期:
2015-06-30

文章信息/Info

Title:
Growth and immunity of Carassius gibelio in response to Bacillus coagulans JSSW-07
作者:
张维娜12; 夏飞1; 谢骏3; 高亮1; 孙梅1; 陈秋红1; 匡群1; 邹苏艳4; 张宪中5
（1．江苏省苏微微生物研究有限公司，江苏无锡214063；2.南京农业大学无锡渔业学院，江苏无锡214081；3.中国水产科学研究院淡水渔业研究中心，江苏无锡214081；4.江苏宜兴天石饲料有限公司，江苏宜兴214200；5.无锡市水产技术指导站，江苏无锡214000）
Author(s):
ZHANG Wei-na12; XIA Fei1; XIE Jun3; GAO Liang1; SUN Mei1; CHEN Qiu-hong1; KUANG Qun1; ZOU Su-yan4; ZHANG Xian-zhong5
(1.Jiangsu Suwei Microbiology Research Co.〖KG-*3〗, Ltd.〖KG-*3〗，Wuxi 214063, China;2.Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;3.Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;4.Jiangsu Yixing Tianshi Feed Co.〖KG-*3〗, Ltd.〖KG-*3〗, Wuxi 214200, China;5.Wuxi Aquatic Technology Extension Station, Wuxi 214063, China)
关键词:
异育银鲫; 凝结芽孢杆菌; 生长; 免疫
Keywords:
Carassius gibelio; Bacillus coagulans; growth; immunity
分类号:
S965.117
DOI:
10.3969/j.issn.1000-4440.2015.03.027
文献标志码:
A
摘要:
为研究在饲料中添加不同含量的凝结芽孢杆菌对异育银鲫幼鱼生长、免疫功能的影响，选取平均体质量为 (17.70±0.07) g的健康异育银鲫幼鱼随机分为4组，3组试验组饲料中分别添加质量分数为0.1%、0.3%、0.5%的凝结芽孢杆菌菌粉（含菌量为 5.0×108〖KG*4〗CFU/g），对照组饲料中不添加凝结芽孢杆菌。试验结果表明：（1）在饲料中添加0.3%、0.5%凝结芽孢杆菌能够显著提高鱼体的增质量率（WGR）、特定增长率（SGR）（P<0.05）,并能够显著降低饵料系数（FCR）（P<0.05）。（2）各试验组的白细胞（WBC）、红细胞(RBC)数量和血红蛋白(HGB)浓度均无显著差异（P>0.05）。（3）添加0.5%凝结芽孢杆菌能显著提高碱性磷酸酶（ALP）、谷草转氨酶（AST）活性及总蛋白质（TP）含量（P<0.05）；添加0.3%凝结芽孢杆菌能显著降低碱性磷酸酶（ALP）、谷丙转氨酶（ALT）活性（P<0.05）；添加0.1%凝结芽孢杆菌能显著降低碱性磷酸酶（ALP）活性（P<0.05）；添加凝结芽孢杆菌对葡萄糖（GLU）、总胆固醇（TC）、甘油三酯（TG）含量无显著影响。（4）添加0.3%、0.5%凝结芽孢杆菌能显著提高肝脏的总抗氧化能力（T-AOC）及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性（P<0.05），有效抑制丙二醛（MDA）的生成；添加0.1%凝结芽孢杆菌能显著提高肝脏的过氧化氢酶活性（P<0.05），对肝脏的抗氧化能力、超氧化物歧化酶活性无显著影响（P>0.05），不能抑制丙二醛的生成。可见，饲料中添加0.3%凝结芽孢杆菌可较好地促进异育银鲫幼鱼的生长，提高机体免疫力。
Abstract:
The effects of different supplement levels of Bacillus coagulans on growth performance and immunity function of juvenile Carassius gibelio were studied in experiment. The fish with an initial average body weight of (17.70±0.07) g were randomly divided into four groups with 3replicates per group and 30 fish per replicate. The fish in the control group were fed with basal diet, and three experimental groups were fed with basal diet supplemented with B. coagulans at the dose of 5.0×108 CFU/g (mass fraction levels 0.1%, 0.3%, 0.5%, respectively). The experiment lasted for 45 d. The weight gain rate (WRG) and specific growth rate (SGR) of the fish feeding on 0.3% and 0.5% B. coagulans diets were significantly higher than those of control （P<0.05）, and the feed conversion rate (FCR) was significantly lower than that of control （P<0.05）. The white blood cell count (WBC), red blood cell count (RBC), hemoglobin content (HGB) had no significant difference （P>0.05） among groups. Compared with control, the content of total protein (TP) and the activities of ALP and AST of the fish feeding on 0.5% B. coagulans diet increased significantly （P<0.05）, the activities of ALP and alanine ALT of the fish feeding 0.3% B. coagulans diet decreased significantly （P<0.05）, and the activity of ALP of the fish feeding on 0.1% B. coagulans diet decreased significantly （P<0.05）. The contents of glucose, total cholesterol (TC), total triglyceride (TG) were not affected by B. coagulans supplementation （P>0.05）. Compared with control, the total antioxidant capacity (T-AOC) and the activities of superoxide dismutase (SOD) and catalase (CAT) were significantly improved in the fish feeding on 0.3% and 0.5% B. coagulans diets （P<0.05）, and the accumulation of malonaldehyde (MDA) was effectively inhibited in the two experimental groups. The supplementation of 0.1% B. coagulans improved liver catalase (CAT) activity （P<0.05）, but did not affect the activities of SOD and T-AOC （P>0.05）, and did not inhibit the generation of MDA. In conclusion, the diet supplemented with 0.3% B. coagulans can promote the growth of juvenile Carassius auratus gibelio and enhance the immunity function.

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备注/Memo

备注/Memo:
收稿日期：2014-12-23 基金项目：江苏省水产三新工程项目（Y2014-3） 作者简介：张维娜(1985-)，女，江苏无锡人，本科，助理研究员，研究方向为应用微生物。（E-mail）zhangweina4045@163.com 通讯作者：谢骏，（E-mail）87838718@qq.com
更新日期/Last Update: 2015-06-30