[1]周艳红,付言峰,赵为民,等.胚胎附植期梅山猪EphrinA1全编码区克隆及生物信息学分析[J].江苏农业学报,2015,(02):350-356.[doi:10.3969/j.issn.1000-4440.2015.02.020]
 ZHOU Yan-hong,FU Yan-feng,ZHAO Wei-min,et al.Coding sequence cloning and bioinformatics analysis of EphrinA1 during embryo implantation in Meishan swine[J].,2015,(02):350-356.[doi:10.3969/j.issn.1000-4440.2015.02.020]
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胚胎附植期梅山猪EphrinA1全编码区克隆及生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
350-356
栏目:
畜牧兽医·水产养殖
出版日期:
2015-04-30

文章信息/Info

Title:
Coding sequence cloning and bioinformatics analysis of EphrinA1 during embryo implantation in Meishan swine
作者:
周艳红12付言峰2赵为民2 刘红林1任守文12
(1.南京农业大学动物科技学院,江苏南京210095;2.江苏省农业科学院畜牧研究所/动物品种改良和繁育重点实验室,江苏南京210014)
Author(s):
ZHOU Yan-hong12FU Yan-feng2ZHAO Wei-min2LIU Hong-lin1REN Shou-wen12
(1.College of Animal Science and Technology,Nanjing Agricultural University, Nanjing 210095,China;2.Institute of Animal Science,Jiangsu Academy of Agricultural Sciences/Laboratory of Animal Improvement and Reproduction, Nanjing 210014,China)
关键词:
梅山猪胚胎附植EphrinA1克隆生物信息学
Keywords:
Meishan swineembryo implantationEphrinA1cloningbioinformatics
DOI:
10.3969/j.issn.1000-4440.2015.02.020
文献标志码:
A
摘要:
为了研究促红细胞生成素产生肝细胞配体A1(EphrinA1)对梅山猪胚胎附植期胚胎在子宫内膜间迁移和对子宫内膜黏附活动的影响,利用克隆测序、生物信息学和qRT-PCR方法,克隆了EphrinA1基因的全长编码序列,对其表达的蛋白结构和功能进行了预测,并检测了其mRNA表达变化。梅山猪EphrinA1基因编码区有5个cSNPs变异,其中2个导致氨基酸突变,分别为Glu29Asn和Trp33Arg,位于EphrinA1外显子1和外显子2上。EphrinA1编码序列中含5个外显子,编码205个氨基酸。EphrinA1为不稳定的亲水性蛋白,有1个信号肽和一个保守区域,无跨膜区。另外,EphrinA1在梅山猪胚胎附植期有较强的mRNA表达。表明EphrinA1基因可能参与梅山猪的胚胎附植调控,其cSNPs有望成为影响猪产仔数基因的潜在分子标记。
Abstract:
In order to analyze the role of EphrinA1 in the migration and adhesion of embryo along the endometrium during embryo implantation in Meishan swine, EphrinA1 coding sequence was cloned, protein structure and function were predicted and mRNA expression was detected by cloning, sequencing, bioinformatics and qRT-PCR method. Five cSNPs in EphrinA1 coding sequence were detected, two of which caused two amino acids changes which were Glu29Asn in exon 1 and Trp33Arg in exon 2. EphrinA1 coding sequence contains five exons and encods 205 amino acids. Protein EphrinA1 is hydrophilic and instable, and has a signal peptide and a conservative region and no transmembrane region. EphrinA1 mRNA showed a strong expression in Meishan swine during embryo implantation period. The results suggested that EphrinA1 might play a role in the regulation of embryo implantation in Meishan swine, and the cSNP is a potential molecular marker affecting swine litter size. 

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备注/Memo

备注/Memo:
收稿日期:2014-07-18 基金项目:国家自然科学基金项目(31201767);江苏省农业科技自主创新基金项目[CX(13)5042];国家生猪现代产业技术体系南京综合试验站项目(nycytx-009);江苏省科技支撑计划项目(BE2012333) 作者简介:周艳红(1987-),女,甘肃白银人,硕士研究生,研究方向为动物遗传育种与繁殖。(Tel)025-84391941;(E-mail)954268869@qq.com。付言峰为共同第一作者。 通讯作者:刘红林,(E-mail)liuhonglin@njau.edu.cn;任守文,(Tel)025-84390349;(E-mail)shouwenren@163.com
更新日期/Last Update: 2015-04-30