[1]程超,沈加飞,明庆磊.赤拟谷盗和杂拟谷盗体内Wolbachia的感染状况和种间传播[J].江苏农业学报,2015,(02):272-278.[doi:10.3969/j.issn.1000-4440.2015.02.008]
 CHENG Chao,SHEN Jia-fei,MING Qing-lei.Infection and interspecific transmission of Wolbachia in Tribolium castaneum and T. confusum[J].,2015,(02):272-278.[doi:10.3969/j.issn.1000-4440.2015.02.008]
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赤拟谷盗和杂拟谷盗体内Wolbachia的感染状况和种间传播()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
272-278
栏目:
植物保护
出版日期:
2015-04-30

文章信息/Info

Title:
Infection and interspecific transmission of Wolbachia in Tribolium castaneum and T. confusum
作者:
程超沈加飞明庆磊
(江苏师范大学生命科学学院,江苏徐州221116)
Author(s):
CHENG ChaoSHEN Jia-feiMING Qing-lei
(School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, China)
关键词:
赤拟谷盗杂拟谷盗沃尔巴克氏体水平传播垂直传播
Keywords:
Tribolium castaneumT. confusumWolbachiahorizontal transmissionvertical transmission
分类号:
S186
DOI:
10.3969/j.issn.1000-4440.2015.02.008
文献标志码:
A
摘要:
为明确沃尔巴克氏体(Wolbachia)在赤拟谷盗和杂拟谷盗体内的感染状况及种间水平和垂直传播情况,首先通过PCR扩增分离细胞分裂蛋白基因(ftsZ)目的片段方法检测这2个近缘种拟谷盗体内是否存在Wolbachia,并将同性别或异性别的赤拟谷盗和杂拟谷盗混合饲养40 d,然后测定赤拟谷盗及其与杂拟谷盗的正反交杂种后代体内Wolbachia的感染状况。结果显示,杂拟谷盗体内存在Wolbachia,而赤拟谷盗体内不存在该菌;混合饲养后,赤拟谷盗及其与杂拟谷盗的正反杂交后代体内也均没有检测到Wolbachia的存在,未发现Wolbachia在杂拟谷盗和赤拟谷盗种间的水平和垂直传播。
Abstract:
To define the infection and interspecifically horizontal and vertical transmission of Wolbachia in two closely related flour beetles Tribolium castaneum (Herbst) and T. confusum (Jac. du Val.), Wolbachia was detected in both beetle species by PCR amplification of ftsZ target gene fragment. After same-sex or opposite-sex mixed feeding, the infection of Wolbachia in the red flour beetles T. castaneum and the reciprocal hybrids of T. castaneum and T. confusum was examined. The wolbachia infected confused flour beetles T. confusum exclusively. The red flour beetles T. castaneum and the reciprocal hybrids were Wolbachia-free. These suggests that interspecifically horizontal and vertical transmissions of Wolbachia did not occur in the two closely related flour beetles T. castaneum and T. confusum, and T. castaneum may have an internal mechanism inhibiting the reproduction and survival of Wolbachia. 

参考文献/References:


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备注/Memo

备注/Memo:
收稿日期:2014-10-28 基金项目:国家自然科学基金项目(31172159);教育部留学回国人员科研启动基金项目[教外司(2012)1707号];江苏师范大学自然科学基金重点项目(13XLA06) 作者简介:程超(1973-),女,江苏徐州人,硕士,副教授,研究方向为昆虫遗传与进化生物学。(Tel)0516-83403171;(E-mail)chengchao@jsnu.edu.cn 通讯作者:明庆磊,(Tel)0516-83403171;(E-mail)mingqinglei@jsnu.edu.cn
更新日期/Last Update: 2015-04-30