[1]陈义,陈健,吕文静,等.硒通过调控小白菜幼苗根内NO 与H2 S 信号诱导BrRbohG1 / BrRbohG2 表达[J].江苏农业学报,2015,(01):149-157.[doi:10.3969/j.issn.1000-4440.2015.01.024]
　CHEN Yi,CHEN Jian,L Wen-jing,et al.Selenium-induced expression of BrRbohG1/BrRbohG2 in Brassica rapa root through nitric oxide and hydrogen sulfide signalling[J].,2015,(01):149-157.[doi:10.3969/j.issn.1000-4440.2015.01.024]
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硒通过调控小白菜幼苗根内NO 与H2 S 信号诱导BrRbohG1 / BrRbohG2 表达()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
149-157
栏目:
园艺
出版日期:
2015-02-28

文章信息/Info

Title:
Selenium-induced expression of BrRbohG1/BrRbohG2 in Brassica rapa root through nitric oxide and hydrogen sulfide signalling
作者:
陈义12; 陈健2; 吕文静12; 李丽娜12; 杨立飞1
（1.南京农业大学园艺学院，江苏南京210095；2.江苏省农业科学院食品质量安全与检测研究所，江苏南京210014）
Author(s):
CHEN Yi 12; CHEN Jian2; L Wen-jing12; LI Li-na12; YANG Li-fei1
（1.College of Horticulture，Nanjing Agricultural University，Nanjing 210095，China；2.Institute of Food Quality Safety and Detection，Jiangsu Academy of Agricultural Sciences， Nanjing 210014，China）
关键词:
小白菜; 亚硒酸钠; 硫化氢; 一氧化氮; BrRbohG1/BrRbohG2
Keywords:
Brassica rapa; selenium; hydrogen sulfide; nitric oxide; BrRbohG1/BrRbohG2
分类号:
S634.3
DOI:
10.3969/j.issn.1000-4440.2015.01.024
文献标志码:
A
摘要:
为了研究硒对农作物胁迫的机理，本试验以小白菜（Brassica rapa chinensis）幼苗为材料，研究了亚硒酸钠（Na2SeO3）处理下，根中内源信号分子一氧化氮（NO）和硫化氢（H2S）的变化与BrRbohG1和BrRbohG2表达的关系。结果表明：0.03～0.46 mmol/L的Na2SeO3显著抑制根的生长，并呈现浓度效应；随着Na2SeO3处理浓度的升高，内源NO含量逐渐上升，而内源H2S逐渐下降，两者呈显著负相关；添加NO合成抑制剂（L-NMMA和Tungstate）或NO清除剂（cPTIO）能够显著抑制Na2SeO3对内源H2S的诱导作用； Na2SeO3处理能够显著诱导根内BrRbohG1和BrRbohG2的表达，分别添加L-NMMA、Tungstate、cPTIO、H2S供体NaHS均能显著抑制Na2SeO3对上述两基因表达的诱导作用。说明Na2SeO3处理下，内源NO介导了Na2SeO3对BrRbohG1和BrRbohG2的诱导表达，而H2S则可能抑制了两个基因的表达; NO也可能作用于H2S信号的上游。
Abstract:
Selenium (Se) has been becoming an emergent pollutant in the agricultural environment. In order to explore the mechanism of Se-induced stress responses in crops, the present study investigated the effect of Na2SeO3 treatment on two important endogenous signalling molecules, hydrogen sulfide (H2S) and nitric oxide (NO), on the roots of Brassica rapa and the regulation of the expression of BrRbohG1 and BrRbohG2 by H2S and NO. Results were achieved as follows. The root growth was significantly inhibited by Na2SeO3 varying from 0.03 mmol/L to 0.46 mmol/L in a dose-dependent manner. Compared to the control group, the root elongation was decreased by almost 60% in the treatment of Na2SeO3 at 0.06 mmol/L. Treatment with Na2SeO3 resulted in the increase in endogenous NO content and the decrease in endogenous H2S content in root in dose-dependent manners. The correlation analysis suggested that there was a significantly negative correlation between NO and H2S in root with Na2SeO3 treatment. The inhibition of H2S induced by 0.06 mmol/L Na2SeO3 could be significantly attenuated by the addition of both NO synthesized inhibitors (L-NMMA and Tungstate) and NO scavenger (cPTIO). The expressions of BrRbohG1 and BrRbohG2 could be induced significantly by Na2SeO3 at 0.06 mmol/L, which could be inhibited by the addition of L-NMMA, Tungstate, cPTIO, and H2S donor sodium hydrosulfide (NaHS), respectively. In conclusion, both endogenous NO and H2S are probably involved in the regulation of the expressions of BrRbohG1 and BrRbohG2 in the root of B. rapa under Na2SeO3 stress. The endogenous NO mediated Na2SeO3-induced expressions of BrRbohG1 and BrRbohG2 while the endogenous H2S might inhibit the expressions of BrRbohG1 and BrRbohG2 in the roots of B. rapa under Na2SeO3 stress. And NO might have functioned the upstream of H2S in the above process.

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备注/Memo

备注/Memo:
收稿日期：2014-08-14 基金项目：国家自然科学基金项目 (31401857)；江苏省农业科技自主创新基金项目 [CX(13)5052] 作者简介：陈义（1990-），男，安徽六安人，硕士研究生，研究方向为蔬菜生理与生物技术。（E-mail）2012104082@njau.edu.cn 通讯作者：杨立飞，(E-mail)lfy@njau.edu.cn
更新日期/Last Update: 2015-02-28