[1]陆玉建,张韩杰,刘南南.大肠杆菌 otsA 基因的克隆和转化本生烟草[J].江苏农业学报,2015,(01):32-38.[doi:10.3969/j.issn.1000-4440.2015.01.005]
　LU Yu-jian,ZHANG Han-jie,LIU Nan-nan.Cloning of otsA gene in Escherichia coli and its transformation into Nicotiana benthamiana[J].,2015,(01):32-38.[doi:10.3969/j.issn.1000-4440.2015.01.005]
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大肠杆菌 otsA 基因的克隆和转化本生烟草()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
32-38
栏目:
遗传育种·耕作栽培·生理生化
出版日期:
2015-02-28

文章信息/Info

Title:
Cloning of otsA gene in Escherichia coli and its transformation into Nicotiana benthamiana
作者:
陆玉建12; 张韩杰1; 刘南南1
（1.滨州学院生命科学系/山东省黄河三角洲野生植物资源开发利用工程技术研究中心，山东滨州256603；2.山东省滨州畜牧兽医研究院，山东滨州256600）
Author(s):
LU Yu-jian12; ZHANG Han-jie1; LIU Nan-nan1
（1.Department of Life Sciences, Binzhou University/Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application in Yellow River Delta, Binzhou 256603, China;2.Binzhou Animal Science & Veterinary Medicine Academy of Shandong Province, Binzhou 256600, China）
关键词:
海藻糖; 大肠杆菌; otsA基因; 本生烟草; 根癌农杆菌
Keywords:
trehalose; Escherichia coli; otsA gene; Nicotiana benthamiana; Agrobacterium tumefaciens
分类号:
Q785;S572
DOI:
10.3969/j.issn.1000-4440.2015.01.005
文献标志码:
A
摘要:
海藻糖是一类重要的渗透调节剂，可以提高植物抵抗多种非生物胁迫的能力。6-磷酸海藻糖合成酶（TPS）是海藻糖生物合成的关键酶，在大肠杆菌中，otsA基因编码TPS。本试验通过PCR技术克隆otsA基因，构建p2300-otsA-GFP表达载体,用农杆菌介导的方法将otsA基因导入到本生烟草中。对筛选获得的转基因植株进行检测表明，otsA基因已成功整合到本生烟草的基因组中，并能进行有效转录。而且研究发现，转基因幼苗在含有150 mmol/L NaCl的培养基中能够生长，具有一定的抗盐胁迫能力。
Abstract:
Trehalose, as an important osmotic regulator, improves plant tolerance to multiple abiotic stress. In Escherichia coli, TPS is a key enzyme for the biosynthesis of trehalose encoded by otsA gene. In this experiment, otsA gene was cloned and transferred into the genome of Nicotiana benthamiana by Agrobacterium-mediated method. The transgenic seedlings in the medium containing 150 mmol/L NaCl grew much better than wild-type seedlings, indicative of the salt tolerance of otsA-transgenic seedlings.

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备注/Memo

备注/Memo:
收稿日期：2014-06-30 基金项目：山东省自然科学基金项目（ZR2012CL14）；滨州学院博士基金项目（2010Y08） 作者简介：陆玉建（1979-），男，河南南阳人，博士，讲师，主要从事细胞工程及分子生物学研究。（Tel）0543-3190096；（E-mail）luyujian79@163.com
更新日期/Last Update: 2015-02-28