[1]付涛,宋帅杰,李文,等.樱花响应水胁迫的转录组分析及相关耐涝基因的挖掘[J].江苏农业学报,2026,42(07):1456-1470.[doi:doi:10.3969/j.issn.1000-4440.2026.07.017]
　FU Tao,SONG Shuaijie,LI Wen,et al.Transcriptome analysis and excavation of related waterlogging tolerance genes of cherry blossom response to water stress[J].,2026,42(07):1456-1470.[doi:doi:10.3969/j.issn.1000-4440.2026.07.017]
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樱花响应水胁迫的转录组分析及相关耐涝基因的挖掘()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年07期
页码:
1456-1470
栏目:
园艺
出版日期:
2026-07-31

文章信息/Info

Title:
Transcriptome analysis and excavation of related waterlogging tolerance genes of cherry blossom response to water stress
作者:
付涛1; 宋帅杰2; 李文1; 刘峰1; 何月秋1; 丰凯1; 刘亮1; 王志龙1
(1.宁波城市职业技术学院,浙江宁波315100；2.汇绿园林建设发展有限公司,浙江宁波315100)
Author(s):
FU Tao1; SONG Shuaijie2; LI Wen1; LIU Feng1; HE Yueqiu1; FENG Kai1; LIU Liang1; WANG Zhilong1
(1.Ningbo City College of Vocational Technology, Ningbo 315100, China；2.Huilü Garden Construction and Development Co., Ltd., Ningbo 315100, China)
关键词:
樱花; 水涝胁迫; 转录组测序; 富集分析; 差异表达基因; 耐涝基因
Keywords:
cherry blossom; waterlogging stress; transcriptome sequencing; enrichment analysis; differentially expressed gene; waterlogging tolerance gene
分类号:
S685.99；Q786
DOI:
doi:10.3969/j.issn.1000-4440.2026.07.017
文献标志码:
A
摘要:
本研究旨在利用转录组分析耐涝种质沼生矮樱与敏涝种质华中樱在水涝胁迫下产生的差异表达基因和代谢路径,挖掘相关耐涝基因,为樱花耐涝品种及砧木的选育提供理论依据。对经历长时间水胁迫处理的材料——华中樱(每3 d浇1次水，HZ)、华中樱(每天浇1次水，XP)和沼生矮樱(每3 d浇1次水，ZS)进行叶片转录组测序,进而对差异表达基因进行功能注释、富集分析及耐涝基因挖掘,并利用实时荧光定量PCR（qRT-PCR）对筛选到的基因进行表达验证。结果表明,通过基因本体论数据库(GO)、京都基因与基因组百科全书数据库(KEGG)、蛋白质直系同源簇数据库(COG)、非冗余蛋白数据库(NR)、瑞士蛋白数据库(Swiss-Prot)、蛋白家族数据库(Pfam)六大功能数据库的联合注释,共鉴定出31 891个已知功能基因。差异表达基因分析结果显示,ZS与HZ比较组、XP与HZ比较组及ZS与XP比较组分别识别到3 670个、2 248个和5 423个差异表达基因。功能富集分析结果显示,在ZS与HZ比较组中,差异表达基因主要显著富集于氨基酸代谢和降解、半乳糖代谢、黄酮类生物合成、硫代谢、萜类物质合成等途径；在XP与HZ比较组中,差异表达基因主要显著富集于氨基酸代谢和降解、淀粉和蔗糖代谢、甘油酯代谢、脂肪酸降解、糖酵解/糖异生、柠檬酸循环、半乳糖代谢、萜类物质合成、抗坏血酸和醛酸代谢、乙醛酸和二羧酸代谢等途径；在ZS与XP比较组中,差异表达基因主要显著富集在半乳糖代谢、类胡萝卜素生物合成、二萜生物合成、氨基酸代谢与降解、甘油酯代谢、脂肪酸降解、黄酮类生物合成、糖酵解/糖异生、抗坏血酸和醛酸代谢、氨基糖和核苷酸糖代谢、淀粉和蔗糖代谢等途径。通过对差异表达基因的分析,在XP与ZS比较组中筛选出与耐涝相关差异表达基因31个,在HZ与ZS比较组中筛选出与耐涝相关差异表达基因5个,在HZ与XP比较组中筛选出与耐涝相关差异表达基因6个。qRT-PCR 验证结果表明,10个与耐涝相关的差异表达基因的表达趋势与转录组测序结果趋势一致。本研究通过长时间水涝胁迫试验,采用转录组分析得出樱花对长时间水涝胁迫的耐受能力主要涉及淀粉和蔗糖代谢、糖酵解/糖异生、黄酮生物合成、脂肪酸降解、抗坏血酸和醛酸代谢等途径,其中涉及的耐涝基因主要有ERF、SUS、RAMY和EXP等4类,这些基因资源可为后续通过基因工程或分子育种手段培育樱花耐涝品种及砧木提供核心基因资源。
Abstract:
This study aims to utilize transcriptome analysis to investigate the differentially expressed genes and metabolic pathways generated under waterlogging stress in the flooding-tolerant germplasm Prunus jingningensis and the flooding-sensitive germplasm Prunus conradinae, and to explore related flooding-tolerant genes, providing a theoretical basis for the breeding of flooding-tolerant varieties and rootstocks. Leaf transcriptome sequencing analysis was conducted for materials subjected to prolonged water stress treatment, Prunus conradinae watered every three days was denoted as HZ, Prunus conradinae watered every day was denoted as XP, and Prunus jingningensis watered every three days was denoted as ZS. Subsequently, functional annotation, enrichment analysis, and flooding-tolerant gene mining were conducted on differentially expressed genes, and the selected genes were verified for expression using quantitative real-time polymerase chain reaction (qRT-PCR). The results showed that a total of 31 891 known functional genes were identified through joint annotation of six functional databases: Gene Ontology Database (GO), Kyoto Encyclopedia of Genes and Genomes Database (KEGG), Clusters of Orthologous Groups Database (COG), Non-redundant Protein Database (NR), Swiss Protein Database (Swiss-Prot), and Protein Family Databases (Pfam). Differential gene expression analysis revealed that 3 670, 2 248, and 5 423 differentially expressed genes were identified in the ZS vs. HZ, XP vs. HZ, and ZS vs. XP comparison groups, respectively. Functional enrichment analysis indicated that in the ZS vs. HZ comparison group, differentially expressed genes were significantly enriched in pathways such as amino acid metabolism and degradation, galactose metabolism, flavonoid biosynthesis, sulfur metabolism, and terpenoids synthesis; in the XP vs. HZ comparison group, they were significantly enriched in pathways such as amino acid metabolism and degradation, starch and sucrose metabolism, glyceride metabolism, fatty acid degradation, glycolysis/gluconeogenesis, citric acid cycle, galactose metabolism, terpenoids synthesis, ascorbic acid and aldehyde acid metabolism, and glyoxylic acid and dicarboxylic acid metabolism; in the ZS vs. XP comparison group, they were significantly enriched in pathways such as galactose metabolism, carotenoids biosynthesis, diterpene biosynthesis, amino acid metabolism and degradation, glyceride metabolism, fatty acid degradation, flavonoid biosynthesis, glycolysis/gluconeogenesis, ascorbic acid and aldehyde acid metabolism, amino sugar and nucleotide sugar metabolism, and starch and sucrose metabolism. Through the analysis of differentially expressed genes, 31 genes related to waterlogging tolerance were identified in the XP and ZS comparison group, five genes in the HZ and ZS comparison group, and six genes in the HZ and XP comparison group were also identified. qRT-PCR verification results indicated that the expression trends of ten differentially expressed genes related to waterlogging tolerance were consistent with the transcriptome sequencing results. Through prolonged waterlogging stress experiments and transcriptome analysis, this study concluded that the tolerance of cherry blossoms to prolonged waterlogging stress mainly involved pathways such as starch and sucrose metabolism, glycolysis/gluconeogenesis, flavonoid biosynthesis, fatty acid degradation, ascorbic acid and aldehyde acid metabolism. The waterlogging-tolerant genes primarily involved four types: ERF, SUS, RAMY, and EXP. These gene resources can provide core gene resources and theoretical support for the subsequent cultivation of waterlogging-tolerant varieties and rootstocks of cherry blossoms through genetic engineering or molecular breeding methods.

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备注/Memo

备注/Memo:
收稿日期:2025-06-06基金项目:宁波市“科创甬江2035”重点技术研发计划项目(2025Z096)；高校国内访问工程师校企合作项目(FG2024302)作者简介:付涛(1988-),男,安徽定远人,硕士,高级实验师,主要从事植物生理生化与分子生物学研究。(E-mail)futao@nbcc.cn通讯作者:王志龙,(E-mail)wangzhil01@163.com
更新日期/Last Update: 2026-08-21